Application of long chain non-coding RNA as marker to Alzheimer's disease detection
An Alzheimer's disease, long-chain non-coding technology, which is applied in the detection/testing of microorganisms, medical preparations containing active ingredients, drug combinations, etc., and can solve problems such as weakening inhibitory effects
Inactive Publication Date: 2018-11-23
孔祥东 +1
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When the cells were infected by influenza virus, herpes simplex virus and stimulated by poly I:C (polyinosine-cytidylic acid), NEAT1 could induce the translocation of SFPQ from the IL-8 gene promoter region to the paraspeckle, and the result Causes excessive formation of paraspeckles, and SFPQ attenuates the repression of this gene
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[0028] 1. Analysis and screening of differentially expressed gene data in patients with Alzheimer's disease
[0029] The data collected from the NCBI-GEO database with the registration number GSE5281 (which contains the data of the corresponding gene expression of AD patients) was analyzed using the NCBI-GEO-Analyze with GEO2R tool, and some of the analysis results are shown in Table 1 . The sequence of probe 227062_AT in Table 1 is shown in SEQ ID NO:3, and the sequence of probe 225239_AT is shown in SEQ ID NO:4.
[0030] From the analysis results, we found that there are a large number of differentially expressed genes in AD patients, among which ITPKB, NEAT1, SLC35E and other genes are up-regulated, and ATP5C1, TUBB, PSMB3 and other genes are down-regulated.
[0031] Table 1. GEO2R analysis results of some genes
[0032] ranking
probe
adjusted p-value
fold change log value
gene name
1
225239_at
9.1E-27
3.72
NEAT1
2
22...
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The invention discloses application of long chain non-coding RNA as a marker to Alzheimer's disease detection. The invention discovers that specific long chain non-coding RNA gene significantly upregulated in expression in patients with Alzheimer's disease, the clinical auxiliary diagnosis technology for the Alzheimer's disease is not perfected at present, the expression of the specific long chainnon-coding RNA in a person with the Alzheimer's disease is significantly upregulated higher than that in a healthy person, which suggests that the specific long chain non-coding RNA has the potentialto be a marker for the diagnosis or auxiliary diagnosis of the Alzheimer's disease, also suggests that the specific long chain non-coding RNA has the potential to be an effective target for the treatment of the Alzheimer's disease, and shows that an inhibitor of the specific long chain non-coding RNA has the potential value of clinical application to the preparation of drug compositions for the treatment of the Alzheimer's disease. The invention provides new medicines and method for the effective treatment of the Alzheimer's disease.
Description
technical field [0001] The invention discloses the application of long-chain non-coding RNA as a detection marker for Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a neurodegenerative disease with insidious onset and progressive development. Clinically, it is characterized by comprehensive dementia such as memory impairment, aphasia, apraxia, agnosia, impairment of visuospatial skills, executive dysfunction, and personality and behavior changes. The etiology is still unknown. Those with onset before the age of 65 are called Alzheimer's disease; those with onset after the age of 65 are called senile dementia. Patients typically live 8 to 10 years after symptoms appear, but the course of the disease can range from 1 to 25 years. Death is usually caused by pneumonia, malnutrition, or general physical exertion (activity). In 2015, the number of dementia patients in the world has reached 46.8 million, of which 50%-75% are Alzheimer's patients, ...
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IPC IPC(8): C12Q1/6883A61K45/00A61P25/28
CPCA61K45/00A61P25/28C12Q1/6883C12Q2600/158C12Q2600/178
Inventor 孔祥东杨宇霞
Owner 孔祥东