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Loaded micropeptide nano liposome and preparation, application and use method thereof

A nano-liposome and liposome technology, which is applied in pharmaceutical formulations, cosmetic preparations, cosmetic preparations and other directions, can solve the problems of encapsulating macromolecular bioactive substances, poor stability of micropeptides, and low bioavailability, etc. To achieve the effect of preventing the reduction of the encapsulation rate, reducing the activity, and simplifying the reaction process

Inactive Publication Date: 2018-11-30
YANGZHOU MENGXIAO BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the combination of the two has not been applied to liposomes, and liposomes are generally large unilamellar liposomes, which are generally large in size and cannot be suitable for encapsulating macromolecular bioactive substances, which is prone to the problem of low encapsulation efficiency , while micropeptides have poor stability in vitro, are easily inactivated, and have low bioavailability

Method used

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  • Loaded micropeptide nano liposome and preparation, application and use method thereof
  • Loaded micropeptide nano liposome and preparation, application and use method thereof
  • Loaded micropeptide nano liposome and preparation, application and use method thereof

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preparation example Construction

[0037] The preparation method of the micropeptide in the present invention comprises the following steps: crushing the animal submandibular gland tissue, adjusting its pH value to weak acidity, and stirring to obtain a homogenate; thawing the homogenate after freezing, and centrifuging; After liquid filtration and ultrafiltration, the micropeptide essence is obtained, and then the micropeptide dry powder is obtained after vacuum drying.

[0038] In the present invention, lecithin and cholesterol are used to prepare the liposome membrane, the lecithin makes the liposome have a lower phase transition temperature, and the cholesterol makes the liposome have good fluidity and low viscosity.

Embodiment 1

[0041] (1) Add 150 parts of lecithin and 30 parts of cholesterol into the rotary evaporating flask, then add 3 times the amount of lecithin and chloroform of the total amount of cholesterol in the rotary evaporating flask, stir and dissolve, stir for 1 to 2 hours and then evaporate under reduced pressure, Until chloroform is fully reclaimed, the liposome membrane is obtained;

[0042] (2) dissolving the liposome membrane in ether, and using microwaves to make a single-chamber liposome solution A with an average particle diameter of 40-400 nm;

[0043](3) Add heavy distilled deionized water to 30 parts of micropeptides, then add 30 parts of Gynostemma pentaphyllum extract and 1 part of sodium hyaluronate, stir and mix evenly to make composite micropeptide solution B, and slowly dissolve composite micropeptide solution B Add to single-chamber liposome solution A, add butanediol and propylene glycol at the same time, mix evenly, hydrate at 40-50°C for 1-2 hours, cool down to -20°...

Embodiment 2

[0049] (1) Add 200 parts of lecithin and 60 parts of cholesterol into a rotary evaporator, then add 3 times the amount of chloroform of the total amount of raw materials into the rotary evaporator and stir to dissolve, stir for 1 to 2 hours and then evaporate under reduced pressure until the chloroform is completely recovered , making liposome membrane;

[0050] (2) dissolving the liposome membrane in ether, and using microwaves to make a single-chamber liposome solution A with an average particle diameter of 40-400 nm;

[0051] (3) Add heavy distilled deionized water to 50 parts of oligopeptides, then add 50 parts of Gynostemma pentaphyllum extract and 10 parts of sodium hyaluronate, stir and mix evenly to make composite oligopeptide solution B, and slowly dissolve composite oligopeptide solution B Add to single-chamber liposome solution A, add butanediol and propylene glycol at the same time, mix evenly, hydrate at 40-50°C for 1-2 hours, cool down to -20°C--10°C for freezing...

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Abstract

The invention relates to loaded micropeptide nano liposome and a preparation, an application and use method thereof, which belong to the technical field of cosmetic. The method utilizes lecithin and cholesterol to prepare nano liposome; and uses the micropeptide, sodium hyaluronate and a gynostemma pentaphyllum extract to prepare micropeptide-encapsulated liposome; the micropeptide-encapsulated liposome is sheared by a homogenizer to prepare a nano-liposome solution; the soluble proteoglycan, glucose, sodium hyaluronate, mannose, nicotinamide and glucuronic acid are added to the nano-liposomesolution, and the loaded micropeptide nano liposome is obtained after freeze drying. The method has an unique packaging method and fully utilizes the comprehensive performance of micropeptide, sodiumhyaluronate and the gynostemma pentaphyllum extract, maximizes the anti-aging effect, at the same time, the encapsulation properties of liposome are used to keep the activity of micropeptide, and convenient preservation and carrying can be realized.

Description

technical field [0001] The invention belongs to the technical field of cosmetics, and in particular relates to a micropeptide-loaded nanoliposome and its preparation, application and usage methods. Background technique [0002] Liposomes are artificially prepared closed vesicular structures that are spontaneously formed in water by the hydrophobic interaction between phospholipid molecules. The functional ingredients are wrapped inside the liposomes. The preparation of liposomes is simple, non-toxic to the body, non-immunogenic, and can enter the body After that, it is mainly phagocytized by grid cells, so the utilization rate of active ingredients can be improved. Micropeptides can stimulate tyrosine phosphorylation of epidermal growth factor receptors to achieve the effect of repairing hyperplasia, thereby replacing aging and dead cells with new ones, making the skin smooth and elastic, and achieving anti-aging effects. Gynostemma pentaphyllum has good anti-aging, anti-ox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/9789A61K8/73A61K8/64A61K8/14A61K8/02A61Q19/00A61Q19/10A61Q19/08
CPCA61K8/0212A61K8/14A61K8/64A61K8/735A61Q19/00A61Q19/08A61Q19/10A61K8/9789
Inventor 成勇成姚斌
Owner YANGZHOU MENGXIAO BIOLOGICAL ENG CO LTD
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