Antibiotic-free feed functional product containing chitosan oligosaccharide as well as preparation method and application of antibiotic-free feed functional product

A technology of antibiotic-free feed and chitosan oligosaccharides, which is applied in applications, animal feeds, animal feeds, etc., can solve the problems of unstable performance of chitosan-oligosaccharide-containing antibiotic replacement products, inconspicuous effect of antibiotic replacement, and uneven products. Good ability to inhibit pathogenic microorganisms, promote intestinal health, and repair intestinal mucosa

Pending Publication Date: 2018-12-07
QINGDAO KANGER BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

Most of the current market uses single prebiotics or probiotics to replace antibiotics, but the effect of replacing antibiotics is not obvious
[0004] The preparation of chitosan oligosaccharides in anti-replacing products containing chitosan oligosaccharides is mostly degraded by chemical methods. The method of preparing chitosan oligosaccharides by chemically degrading chitosan has the disadvantages of polluting the environment and uneven products, which indirectly leads to the substitution of chitosan oligosaccharides. Unstable anti-product performance

Method used

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  • Antibiotic-free feed functional product containing chitosan oligosaccharide as well as preparation method and application of antibiotic-free feed functional product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The preparation of chitosan oligosaccharide:

[0033] a. Water and chitosan are added to the enzymolysis tank according to the weight ratio of 8:1. After the feeding is completed, an appropriate amount of concentrated hydrochloric acid is added to dissolve, and it is stirred continuously for 24 hours to obtain acid-decomposed chitosan;

[0034] The chitosan was purchased from Qingdao Yunzhou Biotechnology Co., Ltd., production batch number 170522;

[0035] b. Add chitosanase to the acid-decomposed chitosan, the amount of chitosanase added is 5kU / kg chitosan; the enzymolysis temperature is 60°C, and the enzymolysis time is 18h;

[0036] c. After the enzymolysis is completed, the enzymolysis solution passes through the ceramic ultrafiltration membrane (25L / min flow rate, 1.0MPa pressure cycle until the clear liquid is colorless), and the ceramic nanofiltration membrane concentration and purification (50L / min flow rate, 1.0MPa pressure cycle until the clear liquid does no...

Embodiment 2

[0039] An antibiotic-free feed functional product containing chitosan oligosaccharide is prepared by mixing chitosan oligosaccharide, Enterococcus faecium, Clostridium butyricum and glucose oxidase in a weight ratio of 6:0.8:0.8:1.2.

[0040] The oligochitosan is oligochitosan powder with a molecular weight between 1000Da and 1400Da, wherein the content of oligochitosan is more than or equal to 80%;

[0041] The number of live bacteria of Enterococcus faecium is 1×10 9 CFU / g;

[0042] The viable count of Clostridium butyricum is 1×10 9 CFU / g;

[0043] The enzyme activity of the glucose oxidase is 3000U / g.

[0044] The preparation method of the anti-antibiotic feed functional product containing chitosan oligosaccharide, the steps are:

[0045] Put chitosan oligosaccharide, Enterococcus faecium, Clostridium butyricum, and glucose oxidase into the mixer according to the weight ratio of 6:0.8:0.8:1.2 and mix evenly, pack in paper plastic and composite aluminum foil bags, and s...

Embodiment 3

[0047] An antibiotic-free feed functional product containing chitosan oligosaccharide is prepared by mixing chitosan oligosaccharide, Enterococcus faecium, Clostridium butyricum and glucose oxidase in a weight ratio of 6:1:1:1.

[0048] The oligochitosan is oligochitosan powder with a molecular weight between 1000Da and 1400Da, wherein the content of oligochitosan is more than or equal to 80%;

[0049] The number of live bacteria of Enterococcus faecium is 1×10 9 CFU / g;

[0050] The viable count of Clostridium butyricum is 1×10 9 CFU / g;

[0051] The enzyme activity of the glucose oxidase is 3000U / g.

[0052] The preparation method is the same as in Example 2.

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Abstract

The invention discloses an antibiotic-free feed functional product containing chitosan oligosaccharide as well as a preparation method and application of the antibiotic-free feed functional product, and belongs to the technical field of feed additives. The antibiotic-free feed functional product containing chitosan oligosaccharide is prepared by mixing chitosan oligosaccharide with enterococcus faecium, clostridium butyricum and glucose oxidase in the weight ratio of 6 to (0.8-1.2) to (0.8-1.2) to (0.8-1.2). The chitosan oligosaccharide in the antibiotic-free feed functional product containingchitosan oligosaccharide can promote proliferation of beneficial microorganisms, the clostridium butyricum can generate butyric acid, lactic acid, acetic acid and the like to restrain growth of harmful bacteria in intestines, the enterococcus faecium cooperate with the clostridium butyricum to exert the antibacterial performance, and the glucose oxidase consumes oxygen, generates hydrogen peroxide, provides anaerobic environment and restores the proportion of intestinal flora. The components can enable the intestines to be healthy for promoting growth through the principle of bacteria resisting, oxygen consumption and repair, so that antibiotics in the intestines are replaced.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to an antibiotic-free feed functional product containing chitosan oligosaccharides, a preparation method and application thereof. Background technique [0002] The use of antibiotics in feed exceeds the standard, resulting in antibiotic residues in farmed animals, which seriously threatens human health. Agriculture has issued three consecutive policies and plans related to the "restriction / prohibition" of antibiotics, from the banning of four types of sandacin drugs to the banning of colistin sulfate, and then to the "National Action Plan for Containing Antimicrobial Resistance of Bacteria of Animal Origin". The determination to address the issue of antibiotic abuse in the livestock industry has been demonstrated. Therefore, it is a problem that people must solve to study novel green additives without antibiotics and high efficiency. [0003] There are many anti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K20/163A23K10/18A23K20/189
CPCA23K10/18A23K20/163A23K20/189
Inventor 任慧慧李丽杰邹圣灿刘泽华
Owner QINGDAO KANGER BIOLOGICAL ENG CO LTD
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