Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions

A technology of chromium ions and sensors, applied in the field of biosensors

Active Publication Date: 2018-12-07
CHINA AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods rely on large-scale instruments and dedicated personnel to operate, and are limited in on-site and field detection, requiring complex pretreatment of samples. Therefore, it is urgent to develop non-polluting, simple, fast, high-sensitivity and high-specificity methods to Meet the needs of trace metal detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions
  • Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions
  • Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1 Establishment of chromium ion-cutting general-purpose barrier ultrafast amplification visualization sensor

[0082] 1. Experimental materials

[0083] SYBR Gold nucleic acid dye, nucleic acid molecular weight standard ultra-low range DNA ladder, dNTP, ExTaq DNA polymerase, 10×Taq buffer, hemin, chromium chloride, 2,2-azino-bis(3-ethyl- Benzothiazole-6-sulfonic acid) diamine salt (ABTS), H2O2, morpholineethanesulfonic acid monohydrate (MES), potassium chloride, sodium chloride, magnesium chloride, potassium hydrogen phosphate, disodium edetate , urea, were purchased from Thermo Scientific Life Technologies (Thermo Scientific Life Technologies). All experimental water was from Milli-Q pure water system.

[0084] The sequence design is as follows (SEQ ID NO:1-5):

[0085]

[0086] Note: The ribozyme cuts the target product and the 3' end sequence of the amplified template is complementary;

[0087] The bold base sequence added to the end of the ribozyme su...

Embodiment 2

[0106] The specificity investigation of embodiment 2 sensor

[0107] According to the biosensor constructed in Example 1, 1nM Cr 3+ , 10 µM Cu 2+ , Zn 2+ 、Cd 2+ , Hg 2+ 、Na + 、Ag + , added to the system for detection, the results show that the established Cr 3+ The biosensor has good specificity ( Figure 4 ).

Embodiment 3

[0108] Embodiment 3 standard addition experiment

[0109] Get high-purity water and detect with the biosensor constructed in embodiment 1, Cr 3+ No detection was carried out, and the standard addition experiment was carried out on it, and the results obtained by continuous measurement are shown in Table 1.

[0110] Table 1 Cr 3+ Spike recovery test results

[0111]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system and (3) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can berealized in the presence of chromium ions, and an amplification product is allowed to form a G-quadruplex in a proper environment; and color development is performed by utilizing the peroxidase-likeactivity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of chromium ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to chromium ions, and is more objective and accurate in detection results.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a chromium ion-cutting type universal partition ultrafast amplification visualization sensor. Background technique [0002] Chromium element symbol Cr, silver-white metal, belongs to group VIB in the periodic table of elements, the atomic number of chromium is 24, the atomic weight is 51.996, body-centered cubic crystal, and the common valences are +3, +6 and +2. The average content of chromium in the earth's crust is 0.010% to 0.011%, and the distribution is extremely uneven. my country's chromium resources are not abundant, and trivalent chromium is one of the indispensable trace elements for the human body. Adults need about 500-700 micrograms of chromium per day. When chromium is deficient, it is easy to make the human body suffer from diabetes and coronary atherosclerosis, which affects the fat metabolism in the body. However, too much chromium can also create pollution...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2521/337C12Q2525/186C12Q2565/607
Inventor 罗云波许文涛黄昆仑杜再慧田晶晶
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com