Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions

A technology of chromium ions and sensors, applied in the field of biosensors

Active Publication Date: 2018-12-07
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods rely on large-scale instruments and dedicated personnel to operate, and are limited in on-site and field detection, requiring complex pretreatment of samples. Therefore, it is urgent to develop non-polluting, simple, fast, high-sensitivity and high-specificity methods to Meet the needs of trace metal detection

Method used

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  • Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions
  • Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions
  • Cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Example 1 Establishment of chromium ion-cutting general-purpose barrier ultrafast amplification visualization sensor

[0082] 1. Experimental materials

[0083] SYBR Gold nucleic acid dye, nucleic acid molecular weight standard ultra-low range DNA ladder, dNTP, ExTaq DNA polymerase, 10×Taq buffer, hemin, chromium chloride, 2,2-azino-bis(3-ethyl- Benzothiazole-6-sulfonic acid) diamine salt (ABTS), H2O2, morpholineethanesulfonic acid monohydrate (MES), potassium chloride, sodium chloride, magnesium chloride, potassium hydrogen phosphate, disodium edetate , urea, were purchased from Thermo Scientific Life Technologies (Thermo Scientific Life Technologies). All experimental water was from Milli-Q pure water system.

[0084] The sequence design is as follows (SEQ ID NO:1-5):

[0085]

[0086] Note: The ribozyme cuts the target product and the 3' end sequence of the amplified template is complementary;

[0087] The bold base sequence added to the end of the ribozyme su...

Embodiment 2

[0106] The specificity investigation of embodiment 2 sensor

[0107] According to the biosensor constructed in Example 1, 1nM Cr 3+ , 10 µM Cu 2+ , Zn 2+ 、Cd 2+ , Hg 2+ 、Na + 、Ag + , added to the system for detection, the results show that the established Cr 3+ The biosensor has good specificity ( Figure 4 ).

Embodiment 3

[0108] Embodiment 3 standard addition experiment

[0109] Get high-purity water and detect with the biosensor constructed in embodiment 1, Cr 3+ No detection was carried out, and the standard addition experiment was carried out on it, and the results obtained by continuous measurement are shown in Table 1.

[0110] Table 1 Cr 3+ Spike recovery test results

[0111]

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Abstract

The invention provides a cleavage type universal-partition ultrafast-amplification visual sensor for chromium ions. The visual sensor comprises (1) a cleavage system, (2) a sPCR amplification system and (3) a detection system containing an ABTS developing solution. According to the invention, primers, a template and probes are ingeniously designed, so ultrafast amplification of the template can berealized in the presence of chromium ions, and an amplification product is allowed to form a G-quadruplex in a proper environment; and color development is performed by utilizing the peroxidase-likeactivity of the G-quadruplex. Thus, the problem of difficulty in realizing visual detection of traditional PCR products is overcome, and rapid and visual detection of chromium ions is realized. Moreover, the sensor provided in the invention has the characteristics of high specificity and high sensitivity to chromium ions, and is more objective and accurate in detection results.

Description

technical field [0001] The invention relates to the technical field of biosensors, in particular to a chromium ion-cutting type universal partition ultrafast amplification visualization sensor. Background technique [0002] Chromium element symbol Cr, silver-white metal, belongs to group VIB in the periodic table of elements, the atomic number of chromium is 24, the atomic weight is 51.996, body-centered cubic crystal, and the common valences are +3, +6 and +2. The average content of chromium in the earth's crust is 0.010% to 0.011%, and the distribution is extremely uneven. my country's chromium resources are not abundant, and trivalent chromium is one of the indispensable trace elements for the human body. Adults need about 500-700 micrograms of chromium per day. When chromium is deficient, it is easy to make the human body suffer from diabetes and coronary atherosclerosis, which affects the fat metabolism in the body. However, too much chromium can also create pollution...

Claims

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Application Information

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IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2521/337C12Q2525/186C12Q2565/607
Inventor 罗云波许文涛黄昆仑杜再慧田晶晶
Owner CHINA AGRI UNIV
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