Molecular marker closely linked with sesame recessive genic male sterility line D248A fertility site and application thereof

A recessive nuclear sterility, D248A technology, applied in the field of sesame molecular breeding, can solve the problems of long cycle, inability to identify fertility, heavy breeding workload, etc., and achieve the effect of improving selection efficiency and speeding up the pace of genetic improvement

Active Publication Date: 2018-12-11
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a molecular marker closely linked with the fertility locus of the sesame recessive genetic male sterile line D248A and its amplification primers, the marker is closely linked with the sterile gene Sims3, the genetic distance is 1.47cM, Using the molecular markers to carry out assisted breeding of hybrid varieties solves the problems of inability to identify fertility in the early early generations, heavy breeding workload, and long cycle in conventional breeding methods, and improves the pace of breeding new hybrid varieties of sesame

Method used

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  • Molecular marker closely linked with sesame recessive genic male sterility line D248A fertility site and application thereof
  • Molecular marker closely linked with sesame recessive genic male sterility line D248A fertility site and application thereof
  • Molecular marker closely linked with sesame recessive genic male sterility line D248A fertility site and application thereof

Examples

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Embodiment 1

[0027] Example 1: Acquisition of closely linked molecular markers for recessive genital sterility in sesame

[0028] In order to better understand the present invention, the present invention takes sesame recessive genic male sterile line D248A and maintainer line D248B as examples to construct a NIL fertility segregation population, and details the method for obtaining molecular markers closely linked with male sterility. However, it should be understood that the above examples do not constitute any limitation to the present invention. In the following experimental steps, unless otherwise specified, all operations were carried out in accordance with the methods provided in "Molecular Cloning Experiment Guide" (Third Edition) (translated by Huang Peitang et al., Beijing: Science Press, 2002). The specific method is as follows:

[0029] (1) Group construction:

[0030] A NIL fertility segregation population with a total of 372 individuals was constructed by crossing the sesam...

Embodiment 2

[0060] Example 2: Application of Sesame Recessive Genic Sterile Molecular Markers in Breeding

[0061] The application of a molecular marker of sesame male sterility in the cultivation of sesame male sterile lines comprises the following steps:

[0062] (1) Breeding population construction

[0063] F 1 , backcross with the parent to get BC 1 f 1 , from which good heterozygous individual plants were self-crossed to obtain BC 1 f 2 , select recessive homozygous individual plants and heterozygous individual plants from the offspring for brother-sister crossing, and the fertility segregation ratio of the offspring is stable at 1:1, which is a new sterile line ( Figure 4 ).

[0064] (2) Fertility identification, DNA extraction and molecular marker detection

[0065] Pollen fertility identification, DNA extraction and marker analysis are the same as in Example 1, please refer to steps (1) to (4) for details. Refer to step (6) for the sequence of primers used for the specifi...

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Abstract

The invention discloses a molecular marker closely linked with a sesame recessive genic male sterility line D248A fertility site and application thereof. The molecular marker is closely linked with asterility gene Sims3, the genetic distance is 1.47 cm, amplification primers of the molecular marker comprise a forward primer 5'-GTTTCCGACCACGCTTCCTA-3' and a reverse primer 5'-GCTCGTCAATGGAGCTAGGG-3', when the primers of the molecular marker are utilized for amplification, a characteristic band of the fertile character is 320 bp, and a characteristic band of the sterile character is 300 bp. According to the molecular marker and the application thereof, in the breeding work of the sesame recessive genic male sterility line, early-stage identification and auxiliary selection are carried out through the molecular marker, a target single plant can be selected at an early generation, the breeding population is remarkably reduced, and therefore the subsequent fertility identification workloadis greatly reduced, and the selection efficiency and accuracy are effectively improved.

Description

technical field [0001] The invention belongs to the field of sesame molecular breeding, and in particular relates to a molecular marker closely linked with the sterility locus of a recessive sesame line D248A and its application in breeding the sesame line. Background technique [0002] Sesame is an important oil crop in the world. In 2016, the global planting area exceeded 10.58 million hectares, with a total output of 6.11 million tons. my country is the fourth largest sesame planting country in the world, with an annual planting area of ​​about 400,000 hectares and a total output of about 600,000 tons. It is a traditional export crop. The oil content of sesame seeds is as high as 50-60%. Sesame oil has pure fragrance and high nutritional value, and is known as the "king of oils". Sesame seeds and sesame oil are important raw materials for the food industry. They can be used to make cakes, candies, canned food, sesame paste, margarine, margarine, sesame tofu, sesame milk ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 刘红艳赵应忠周芳周婷杨远霄
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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