Molecular marker closely linked with sesame recessive genic male sterility line D248A fertility site and application thereof

Abstract

The invention discloses a molecular marker closely linked with a sesame recessive genic male sterility line D248A fertility site and application thereof. The molecular marker is closely linked with asterility gene Sims3, the genetic distance is 1.47 cm, amplification primers of the molecular marker comprise a forward primer 5'-GTTTCCGACCACGCTTCCTA-3' and a reverse primer 5'-GCTCGTCAATGGAGCTAGGG-3', when the primers of the molecular marker are utilized for amplification, a characteristic band of the fertile character is 320 bp, and a characteristic band of the sterile character is 300 bp. According to the molecular marker and the application thereof, in the breeding work of the sesame recessive genic male sterility line, early-stage identification and auxiliary selection are carried out through the molecular marker, a target single plant can be selected at an early generation, the breeding population is remarkably reduced, and therefore the subsequent fertility identification workloadis greatly reduced, and the selection efficiency and accuracy are effectively improved.

Description

technical field [0001] The invention belongs to the field of sesame molecular breeding, and in particular relates to a molecular marker closely linked with the sterility locus of a recessive sesame line D248A and its application in breeding the sesame line. Background technique [0002] Sesame is an important oil crop in the world. In 2016, the global planting area exceeded 10.58 million hectares, with a total output of 6.11 million tons. my country is the fourth largest sesame planting country in the world, with an annual planting area of ​​about 400,000 hectares and a total output of about 600,000 tons. It is a traditional export crop. The oil content of sesame seeds is as high as 50-60%. Sesame oil has pure fragrance and high nutritional value, and is known as the "king of oils". Sesame seeds and sesame oil are important raw materials for the food industry. They can be used to make cakes, candies, canned food, sesame paste, margarine, margarine, sesame tofu, sesame milk ...

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a molecular marker closely linked with the fertility locus of the sesame recessive genetic male sterile line D248A and its amplification primers, the marker is closely linked with the sterile gene Sims3, the genetic distance is 1.47cM, Using the molecular markers to carry out assisted breeding of hybrid varieties solves the problems of inability to identify fertility in the early early generations, heavy breeding workload, and long cycle in conventional breeding methods, and improves the pace of breeding new hybrid varieties of sesame

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