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Application of SAA1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis

A technology for allergic purpura and purpura nephritis, applied in the field of medical diagnosis, to achieve high sensitivity and specificity

Active Publication Date: 2018-12-11
ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No significant association between SAA1 and Henoch-Schonlein purpura nephritis has been found in the prior art

Method used

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  • Application of SAA1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis
  • Application of SAA1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis
  • Application of SAA1 detection agent in preparation of kit for diagnosing Henoch-Schonlein purpura nephritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1iTRAQ Screening Differential Proteins

[0029] Part I. Screening of Urine Molecular Markers of Kidney Injury

[0030] 1. Main experimental materials:

[0031] Kidney damage patients (diabetic AKI patients, AKI+CKD patients, diabetic CKD patients, non-diabetic IgA CKD patients, non-diabetic non-IgA CKD patients, 50 cases each) urine; healthy people (50 cases); iTRAQ kit.

[0032] 2. Experimental setup:

[0033] 2.1 Early detection and inclusion criteria for patients with renal disease

[0034] 2.1.1 AKI inclusion criteria (clinical diagnosis)

[0035] The inclusion criteria for early AKI (AKI stage I) were based on the following two criteria: serum creatinine increased by 26.2 μmol / L or increased to 1.5-1.9 times the baseline value within 48 hours; urine output for more than 6 consecutive hours (less than 12 hours) was lower than 0.5ml / Kg / h.

[0036] 2.1.2 AKI+CKD (AKI patients based on CKD, namely CKD complicated with AKI) inclusion criteria (clinical diag...

Embodiment 2

[0081] Example 2 Results related to SAA1

[0082] According to the protein expression abundance, when the protein difference fold is greater than 1.2 (up-regulation) or less than 0.83 (down-regulation) and p<0.05, the differential protein is selected. The differential proteins screened in each group were sorted according to the fold difference, and the top 10 differential proteins in each group were selected.

[0083] Among them, SAA1 protein was abnormally expressed in the urine of AKI patients complicated with diabetes mellitus, and the up-regulated fold was more than 2.4 times, but there was no significant change in CKD patients complicated with diabetes mellitus.

[0084] Based on this, the protein of interest was obtained by screening and verified by molecular biology.

Embodiment 3

[0085] Example 3 SAA1 is a potential marker for allergic purpura (HSP) nephritis

[0086] 1. Main experimental materials:

[0087] Hematuria (HSP group, AKI type, 50 cases) and kidney puncture samples (7 cases) in patients with HSP renal injury complicated with diabetes mellitus (HSP-non-diabetic group, AKI type, 30 cases) ; patients with diabetes mellitus; chronic kidney injury (CKD) population urine (50 cases) and kidney puncture samples (8 cases); urine (AKI type, 50 cases) and kidney puncture samples from patients with thrombocytopenic purpura (ITP) renal injury Samples (7 cases); urine of healthy people (50 cases). All specimens were collected with the informed consent of the patients and signed informed consent.

[0088] 2. Urine specimen collection:

[0089]The hematuria specimens of the allergic purpura nephritis group, chronic kidney injury and thrombocytopenic purpura group were collected in the early morning of the second day after admission, and the hematuria sp...

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Abstract

The invention relates to a novel marker SAA1 of Henoch-Schonlein purpura nephritis; and application of the novel marker SAA1 is researched. According to the application, clinical experiments prove that changes of SAA1 in blood serum and urine of individuals have relatively high sensitivity and specificity to diagnosis on the Henoch-Schonlein purpura nephritis, and it is found that the marker has obvious difference in allergic purpura, thrombopenic purpura and chronic renal injury. According to the application, a novel means of diagnosis is provided for clinical diagnosis on Henoch-Schonlein purpura (nephropathy), and the application has a certain potential clinical value in assistance of treatment and disease course monitoring of the Henoch-Schonlein purpura (nephropathy).

Description

technical field [0001] The invention relates to the field of medical diagnosis, in particular to the application of a SAA1 detection agent in the preparation of a kit for diagnosing allergic purpura nephritis. Background technique [0002] Henoch-Schonlein purpura (HSP) is an inflammatory disease involving small blood vessels throughout the body. Clinically, it mainly involves skin, gastrointestinal tract, joints, and kidneys, and may also involve organs such as the brain, lungs, and scrotum. , The pathological features are the deposition of immune complexes dominated by immunoglobulin A (IgA) on the walls of the small blood vessels of the affected organs (immunofluorescence), Henoch-Schonlein purpuranephritis (HSPN) It refers to the renal parenchymal damage caused by Henoch-Schonlein purpura. Clinically, the presence of hematuria and / or proteinuria during the course of Henoch-Schonlein purpura (including within 6 months of the onset of Henoch-Schonlein purpura) can be diagn...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6893G01N2800/347
Inventor 何强金娟李一文龚建光赵黎
Owner ZHEJIANG PROVINCIAL PEOPLES HOSPITAL
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