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Application of miR-21 in regulation of ionizing radiation induced epithelial-mesenchymal transition

A mir-21, 1.mir-21 technology, applied in the biological field, can solve problems such as life-threatening, limiting the effective application of radiotherapy, and affecting the quality of life of patients

Inactive Publication Date: 2018-12-14
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because radiation-induced pulmonary fibrosis is an irreversible process, once it occurs clinically, it will seriously affect the quality of life of the patient and even endanger his life, which limits the effective application of radiotherapy

Method used

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  • Application of miR-21 in regulation of ionizing radiation induced epithelial-mesenchymal transition
  • Application of miR-21 in regulation of ionizing radiation induced epithelial-mesenchymal transition
  • Application of miR-21 in regulation of ionizing radiation induced epithelial-mesenchymal transition

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Experimental program
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Embodiment 1

[0060] Example 1, miR-21 regulates radiation-induced epithelial-mesenchymal transition

[0061] 1. Ionizing radiation induces the expression of miR-21

[0062] 1. Normal culture of lung epithelial cells (A549 and Beas 2B cells). Keep cell borders clear, adherent to the wall in normal shape, and free from microbial contamination.

[0063] 2. Inoculate cells. Inoculate the cells in a good state into a 60mm culture dish, and cultivate them to reach a confluence of 60%-80% within 24 hours.

[0064] 3. Irradiate cells. When the inoculated cells reach 60%-80% confluence, 6Gy 60 Co gamma rays irradiate cells.

[0065] 4. Collect cells by Trizol method to extract total RNA. The control group cells (marked as 0h) were harvested, and the cells (marked as 1h, 3h, 6h, 12h, 24h and 48h) were harvested sequentially at 1, 3, 6, 12, 24 and 48 hours after irradiation. Specific steps are as follows:

[0066] (1) Pour off the cell culture medium, and wash the cells 3 times with PBS buffe...

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Abstract

The invention discloses an application of miR-21 in the regulation of ionizing radiation induced epithelial-mesenchymal transition. The invention provides an application of an miR-21 related biomaterial in the preparation of a product for regulating the radiation-induced epithelial-mesenchymal transition. The miR-21 related biomaterial is miR-21, or a substance capable of promoting the expressionof miR-21, or a substance capable of inhibiting the expression of the miR-21; and the miR-21 is mature miR-21 or precursor miR-21. It is disclosed that ionizing radiation induces the increase in the expression of the miR-21 in lung epithelial cells (A549 and Beas-2B), and the induced miR-21 inhibits the expression of a target protein PTEN and activate an Akt pathway in order to promote the occurrence of EMT. Therefore, the miR-21 can become a new biomarker and therapeutic target for treating radiation-induced pulmonary fibrosis.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of miR-21 in regulating epithelial-mesenchymal transition induced by ionizing radiation. Background technique [0002] Genome-wide transcriptome studies have shown that there are a large number of non-coding RNA (non-coding RNA, ncRNA), non-coding RNA refers to RNA molecules in the transcriptome that are not translated into protein, including microRNA (microRNA, miRNA), nuclear RNA Small nuclear RNA (snRNA), small nucleolar RNAs (snoRNA), small interfering RNA (siRNA), RNA interacting with piwi protein (piwi-interacting RNA, piRNA), long Non-coding RNA (long non-codingRNA, LncRNA), etc. miRNA is a class of endogenous, small molecule non-coding RNA with a length of 19-25 nucleotides, which can mediate mRNA repression or dysregulation, and lead to translation inhibition, so it is an important class of gene expression regulation factor. miRNAs were originally discover...

Claims

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Application Information

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IPC IPC(8): C12N15/90C12N13/00A61K45/00A61P39/00A61P11/00
CPCA61K45/00A61P11/00A61P39/00C12N13/00C12N15/90
Inventor 顾永清周平坤刘政朱茂祥刘晓丹
Owner ACADEMY OF MILITARY MEDICAL SCI
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