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Method for improving fruiting rate of Amorphophallus albus and Amorphophallus konjac hybrid pollination

A technology of hybrid pollination and flowering konjac, which is applied in the direction of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of low seed setting rate, achieve high pollination and seed setting rate, solve the problem of flowering period, and increase the storage time.

Active Publication Date: 2018-12-18
ANKANG AGRI SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The seed setting rate of hybridization of white konjac pollen collected in the field and flower konjac is also low

Method used

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  • Method for improving fruiting rate of Amorphophallus albus and Amorphophallus konjac hybrid pollination
  • Method for improving fruiting rate of Amorphophallus albus and Amorphophallus konjac hybrid pollination

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Effect test

Embodiment 1

[0024] A method for improving the hybrid pollination seed setting rate of white konjac and flower konjac, comprising the steps of:

[0025] (1) Collection of white konjac pollen:

[0026] First of all, select white konjac bulbs that have grown for more than 4 years, have full terminal buds, and are disease-free, and sow them in the greenhouse around March; Digging, keeping the underground bulbs, removing the spathe, and exposing the stamens and pistils of the white konjac; finally, the whole plant of the white konjac is placed on a clean kraft paper, and placed in a light incubator to artificially urge powder. The culture temperature of the light incubator is 28°C, the light intensity is 4950Lx, the humidity is 45%, and the culture time is 24h; when the pollen is scattered on the kraft paper, it is transferred to the cryopreservation tube in time, and the pollen amount m in the cryopreservation tube meets : 0<m<1 / 2V, where V is the volume of the cryotube;

[0027] (2) cryopr...

Embodiment 2

[0035] A method for improving the hybrid pollination seed setting rate of white konjac and flower konjac, comprising the steps of:

[0036] (1) Collection of white konjac pollen:

[0037] First of all, select white konjac bulbs that have grown for more than 4 years, have full terminal buds, and are disease-free, and sow them in the greenhouse around March; Digging, keeping the underground bulbs, removing the spathe, and exposing the stamens and pistils of the white konjac; finally, the whole plant of the white konjac is placed on a clean kraft paper, and placed in a light incubator to artificially urge powder. The culture temperature of the light incubator is 28°C, the light intensity is 5000Lx, the humidity is 50%, and the culture time is 36h; when the pollen is scattered on the kraft paper, it is transferred to the cryopreservation tube in time, and the pollen amount m in the cryopreservation tube meets : 0<m<1 / 2V, where V is the volume of the cryopreservation tube;

[003...

Embodiment 3

[0046] A method for improving the hybrid pollination seed setting rate of white konjac and flower konjac, comprising the steps of:

[0047] (1) Collection of white konjac pollen:

[0048] First of all, select white konjac bulbs that have grown for more than 4 years, have full terminal buds, and are disease-free, and sow them in the greenhouse around March; Digging, keeping the underground bulbs, removing the spathe, and exposing the stamens and pistils of the white konjac; finally, the whole plant of the white konjac is placed on a clean kraft paper, and placed in a light incubator to artificially urge powder. The culture temperature of the light incubator is 28.5°C, the light intensity is 5050Lx, the humidity is 55%, and the culture time is 48h; when the pollen is scattered on the kraft paper, it is transferred to the cryopreservation tube in time, and the pollen amount m in the cryopreservation tube meets : 0<m<1 / 2V, where V is the volume of the cryotube;

[0049] (2) cryo...

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Abstract

The invention discloses a method for improving the fruiting rate of Amorphophallus albus and Amorphophallus konjac hybrid pollination. The method comprises the following steps: (1) collecting pollensof the Amorphophallus albus in a manner of indoor artificial pollination acceleration in an illumination incubator; (2) preserving the collected Amorphophallus albus pollens in a cryopreservation manner; (3) recovering the cryopreserved Amorphophallus albus; and (4) selecting and artificially pollinating the fully-ripe Amorphophallus konjac and the recovered Amorphophallus albus, carrying out bagging treatment, and stopping the bagging treatment when the color of ovaries becomes green. Compared with the prior art, the method has the advantages of realization of the amount, the purity and the vitality of Amorphophallus albus pollens being significantly higher than those of pollens naturally collected in the field, solving of the problem of the asynchronous flowering period of the Amorphophallus albus and Amorphophallus konjac, and significant improvement of the fruiting rate of pollination after hybridization.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for improving the hybrid pollination and seed setting rate of white konjac and flower konjac. Background technique [0002] Konjac is a perennial herbaceous plant of the Araceae Konjac genus. It is the only plant rich in glucomannan found so far in nature. Glucomannan has gelling, cohesive, film-forming, soluble, thickening and water-retaining properties. It is widely used in food, chemistry, medicine, environmental protection and other fields. Konjac germplasm resources are abundant, there are about 170 species in the world, and 21 species are endemic to my country. The existing cultivated varieties in China are mainly flower konjac, white konjac and bulbil konjac, and most of them are local population species, and the variety traits are uneven. In addition, The national konjac production areas are still dominated by unimproved original species, rathe...

Claims

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Application Information

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IPC IPC(8): A01H1/02
CPCA01H1/02
Inventor 段龙飞郭邦利覃剑锋蔡阳光陈国爱张百忍刘次鹏王宗方
Owner ANKANG AGRI SCI RES INST
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