Method for establishing audiogenic seizure (AGS) model based on perforation of tympanic membrane

Abstract

The invention discloses a method for establishing an audiogenic seizure (AGS) model based on the perforation of the tympanic membrane, and belongs to the technical field of human ear biomechanics. Themethod comprises selecting rats in a critical period of auditory development, performing auditory brain response (ABR) detection on the selected rats, and determining the hearing reaction threshold by using the ABR wave III reaction threshold as the standard to select the rats with a hearing threshold of less than 40 dB SPL as a standard group; performing perforation operation of the tympanic membrane of the rats in the standard group, and giving the postoperative rats free access to food and water; and performing AGS detection on the postoperative rats at set time intervals after operation,and taking the rats that produced AGS twice as the AGS model rats. According to the invention, the AGS model established by the method for perforating the tympanic membrane with a syringe needle is simple and easy to operate, and has high stability.

Description

Technical field [0001] The invention relates to the technical field of human ear biomechanics, in particular to a method for establishing an auditory convulsion model based on tympanic membrane perforation. Background technique [0002] Audiogenic seizure (AGS) refers to convulsions that are exhibited under strong sound stimulation, such as running wildly, jumping wildly, generalized clonus, or tonic convulsions-clonic behaviors, which are common in rodents. Auditory brain response (ABR) is a short-latency potential and is generally tested with short sounds. It can be combined with other audiology tests to identify the nature of hearing loss, and is most commonly used to check for post-cochlear disease. [0003] Otitis media has become the most common disease in preschool children. Nearly 90% of children under the age of 6 have suffered from otitis media at least once, and the earlier they are affected, the greater the possibility of recurrence. Patients with otitis media will st...

AI Technical Summary

Problems solved by technology

[0006] However, the existing audiogenic convulsion models are basically hereditary epilepsy-susceptible strains, which retain heredity through gene mutation or inbreeding.

Its cost is high, the operation is more complicated, and it is less manipulable for the study of auditory experiments, and it cannot simulate the audiogenic convulsions that occur in the lives of patients with otitis media.

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