Walnut active peptide for improving memory function, and preparation method thereof

A technology for improving memory and active peptides, applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve problems such as mental disorders and irritability, and achieve high safety, improved spatial cognition and learning ability

Inactive Publication Date: 2018-12-18
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main treatment drugs for patients with memory loss are chemically synthesized drugs such as donepezil hydrochloride and piracetam. Although they can improve the spatial l

Method used

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  • Walnut active peptide for improving memory function, and preparation method thereof
  • Walnut active peptide for improving memory function, and preparation method thereof
  • Walnut active peptide for improving memory function, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of walnut defatted protein powder

[0026] Take 50g of degreased walnut meal and crush it with a multifunctional pulverizer for 40s, mix it with distilled water according to the ratio of material to liquid 1:6 (mass), homogenize it with a colloid mill for 60s, let it rest for 8h, remove the supernatant, centrifuge at 5000rpm for 10min, and take the precipitate After freezing at -20°C, freeze-dry at -50°C and 25Pa for 24 hours to obtain walnut defatted protein powder.

[0027] (2) Preparation of walnut active peptide

[0028] Dissolve 5g of walnut defatted protein powder in 100mL of distilled water to prepare a solution with a mass fraction of 5%, bathe in 90°C for 10 minutes, adjust the pH of the solution to 8 with 1.0mol / L NaOH, cool to 40°C; keep the pH value constant, add alkaline protease Alcalase 2.4L, the amount of enzyme added is 7000U / g, after enzymolysis at 40°C for 3h, the solution is inactivated at 90°C for 10min; cooled to 40°C, and the pH o...

Embodiment 2

[0031] Embodiment 2: Behavioral test research of the present invention on mice

[0032] 1. Test animals: SPF grade Kunming mice, weighing 18-20 g, 60 mice, provided by Changchun Institute of Biological Products Co., Ltd.

[0033] 2. Experimental grouping

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Abstract

The invention discloses a preparation method of a walnut active peptide for improving the memory function, and belongs to the technical field of active peptide preparation. Degreased walnut dregs usedas a raw material are crushed and homogenized, then are fully hydrolyzed by an alkaline protease and a neutral protease through a two-step controlled enzymatic hydrolysis technology, and undergoes amembrane separation technology to obtain the polypeptide with having a molecular weight of above 10 kDa, the polypeptide with having a molecular weight of 10~3 kDa and a polypeptide having a molecularweight of below 3 kDa. The method allows the walnut peptides with high hydrolysis degree and high activity to be obtained under the co-action of above two enzymes. The influences of the peptides on improvement of the memory of experimental animals are evaluated by using spatial exploration test, navigation test, step-through test and step-down test in a mouse Morris water maze as indicators. Results show that the small peptide (<3 kDa)with a short peptide chain can improve the spatial cognition and learning ability of dysmnesia mice, is a food-borne peptide, has a high safety, and can be taken for a long term.

Description

technical field [0001] The invention belongs to the technical field of active peptide preparation, and in particular relates to a walnut active peptide prepared from degreased walnut dregs with obvious memory function and a preparation method thereof. Background technique [0002] With the increase of global population aging, the incidence of a series of neurodegenerative diseases, such as Parkinson's disease (PD) and Alzheimer's disease (AD), which are closely related to memory impairment, is increasing year by year. It has brought a heavy burden to the family and society. At present, the main treatment drugs for patients with memory loss are chemically synthesized drugs such as donepezil hydrochloride and piracetam. Although they can improve the spatial learning and memory cognition ability of patients, they will also produce some adverse reactions, such as hallucinations, easily Mental disturbances of irritability and aggressive behavior. Therefore it is particularly im...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34C07K1/14
CPCC07K1/14C07K1/34C12P21/06
Inventor 闵伟红赵凡睿杨行刘春雷方丽王辑
Owner JILIN AGRICULTURAL UNIV
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