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Measurement method for contents of five chemical constituents in northeast bitter herb

A technology of chemical composition and bittern, applied in the field of content determination of five chemical components in Northeast bittern, can solve the problem of incomplete research on Northeast bittern.

Active Publication Date: 2018-12-18
TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

[0003] At this stage, the research on the quality control of bitter cabbage in Northeast China is not comprehensive, and only chlorogenic acid has been reported in the content determination; bitter cabbage in northeast China contains flavonoids, organic acids, polysaccharides, amino acids and volatile oils, etc. components, only controlling one component cannot effectively control its quality, so a more credible and comprehensive quality control method is needed to provide a basis for the quality control of Northeast bitter cabbage

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  • Measurement method for contents of five chemical constituents in northeast bitter herb
  • Measurement method for contents of five chemical constituents in northeast bitter herb
  • Measurement method for contents of five chemical constituents in northeast bitter herb

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Embodiment Construction

[0032] The invention adopts the ultra-high performance liquid chromatography to simultaneously measure the contents of five chemical components of the Northeast bitter vegetable. When performing ultra-high performance liquid chromatography test, the chromatographic conditions are as follows: chromatographic column: a stationary phase using octadecylsilane bonded silica gel; mobile phase: Phase A is 0.01%~1% phosphoric acid-water, preferably 0.05%~ 0.5% phosphoric acid-water, more preferably 0.1% phosphoric acid-water; Phase B is acetonitrile; Gradient elution; Wherein, gradient elution is preferably that A phase accounts for the volume percentage of mobile phase in the following ranges and decreases with time , 0–8 minutes, 90%–81.5%; 8–20 minutes, 81.5%–81.3%; 20–25 minutes, 81.3%–81.3%; 25–35 minutes, 81.3%–60%; 35–40 minutes, 60%–40%; 40–50 minutes, 40%–25%. The inventor unexpectedly found that adopting the above chromatographic conditions can be very simple, convenient an...

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Abstract

The embodiment of the invention provides a measurement method for contents of five chemical constituents in northeast bitter herb. The chemical constituents comprise chlorogenic acid, isoquercitrin, galuteolin, luteolin and caffeic acid. The method comprises the steps of (1) building a standard curve of the five chemical constituents; (2) acquiring ultra-high performance liquid chromatography of asample solution; and (3) determining the contents of the five chemical constituents in a sample. The ultra-high performance liquid chromatography is employed, the contents of the five chemical constituents in the northeast bitter herb are simultaneously detected, e method has the characteristics of simplicity, rapidness, high stability, high accuracy and good reproducibility, and a basis is provided for quality control of the northeast bitter herb.

Description

technical field [0001] The present invention relates to the technical field of the content assay method of chemical composition, particularly relate to a kind of content assay method of 5 kinds of chemical constituents in Northeast bitter cabbage. Background technique [0002] Northeast bitter cabbage is the dried whole herb of Ixeris vesicolor DC. It is recorded in "Flora of China" and "Jilin Provincial Drug Standards" (1977 edition). In the 2015 Announcement No. 20 of the Jilin Provincial Food and Drug Administration, the beipaijiang (compositae plant Ixeris vesicolor DC. Chinese bitter vegetable Ixeris chinensis Thund. Dried whole herb) was revised as Northeast bitter vegetable (dried whole herb of Compositae plant Ixeris vesicolor DC.). Chicory is a plant with a bitter and cold nature and taste. It can clear away heat and poison, eliminate carbuncle and expel pus, cool blood and stop bleeding, promote blood circulation and remove blood stasis, protect liver and gallbla...

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Application Information

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IPC IPC(8): G01N30/06G01N30/74
CPCG01N30/06G01N30/74
Inventor 何俊姚广哲欧阳慧子常艳旭马文娟贾琪沈家圆
Owner TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE