In-vitro sterile breeding technology for iphigenia indica

An in-test-tube technology of shiitake mushroom, applied in the field of seedling raising, can solve the problems of low planting survival rate, low reproduction coefficient, and easy degeneration of varieties, and achieves the effects of high planting survival rate, improving reproduction coefficient and avoiding degeneration of varieties.

Inactive Publication Date: 2018-12-25
湖北仙草谷中药材有限公司
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AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a kind of aseptic breeding technology in test tube of Arrowhead mushroom, so as to solve the problems that the current method

Method used

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Embodiment Construction

[0008] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0009] 1. Operating equipment: glassware, such as beakers, petri dishes, etc.; newspapers; test tubes; plastic greenhouses; nurseries; high-temperature sterilization and disinfection devices.

[0010] 2. Operation process:

[0011] 1. Seed collection

[0012] Select mature uncracked capsules, disinfect the surface with 0.1% raw mercury, wash them with sterile water, and dry them with sterile filter paper to absorb excess water. Then, cut the capsules under aseptic conditions and spread the seeds on In a glass vessel containing cul...

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Abstract

The invention relates to an in-vitro sterile breeding technology for iphigenia indica. The in-vitro sterile breeding technology for the iphigenia indica comprises the following steps: (1) seed collection; (2) seed pretreatment; (3) seed panning; (4) germination accelerating culture; and (5) strong seedling domestication. The seeds of the iphigenia indica are pretreated at first, a test-tube seedling culture method is adopted, germination of the seeds of the iphigenia indica is induced by a half amount of adjusted MS culture solution and indoor constant-temperature incubation, two months afterthe seeds of the iphigenia indica are germinated, iphigenia indica seedlings are transplanted to a nursery garden and are cultured into commercial domestication seedlings, the breeding coefficient isincreased, variety degeneration is avoided, in the step of seed panning, high-temperature sterilization is carried by fine sand, pest and disease damage is relieved, common equipment is used, the costfor a tissue culture technology is reduced, technological difficulties are small, and the rate of survival of planting is high.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, in particular to the cultivation of seedlings in test tubes, in particular to an aseptic breeding technology in test tubes of Arrowhead mushrooms. Background technique [0002] Shanzi mushroom, the name of traditional Chinese medicine. It is the dry pseudobulb of Rhododendron orchid, Suanlan or Yunnan Suanlan. The former is commonly known as "Mao Ci Mushroom", while the latter two are commonly referred to as "Hockey Ball". Other names include Jin Deng, Zhu Gu, Wintergreen, Wuyi Grass, etc. Excavated in summer and autumn. Shanzi mushroom is sweet in taste, slightly pungent, and cool in nature. It has the effects of clearing heat and detoxifying, eliminating carbuncle and dissipating stagnation, etc. It is commonly used for carbuncle swollen furunculosis, scrofula with phlegm nucleus, snake bites, lame lumps and lumps. [0003] At present, there are mainly three methods for artific...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈风雷孟凡平余胜梅
Owner 湖北仙草谷中药材有限公司
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