In-vitro sterile breeding technology for iphigenia indica

Abstract

The invention relates to an in-vitro sterile breeding technology for iphigenia indica. The in-vitro sterile breeding technology for the iphigenia indica comprises the following steps: (1) seed collection; (2) seed pretreatment; (3) seed panning; (4) germination accelerating culture; and (5) strong seedling domestication. The seeds of the iphigenia indica are pretreated at first, a test-tube seedling culture method is adopted, germination of the seeds of the iphigenia indica is induced by a half amount of adjusted MS culture solution and indoor constant-temperature incubation, two months afterthe seeds of the iphigenia indica are germinated, iphigenia indica seedlings are transplanted to a nursery garden and are cultured into commercial domestication seedlings, the breeding coefficient isincreased, variety degeneration is avoided, in the step of seed panning, high-temperature sterilization is carried by fine sand, pest and disease damage is relieved, common equipment is used, the costfor a tissue culture technology is reduced, technological difficulties are small, and the rate of survival of planting is high.

Description

technical field

[0001] The invention relates to the technical field of seedling cultivation, in particular to the cultivation of seedlings in test tubes, in particular to an aseptic breeding technology in test tubes of Arrowhead mushrooms. Background technique

[0002] Shanzi mushroom, the name of traditional Chinese medicine. It is the dry pseudobulb of Rhododendron orchid, Suanlan or Yunnan Suanlan. The former is commonly known as "Mao Ci Mushroom", while the latter two are commonly referred to as "Hockey Ball". Other names include Jin Deng, Zhu Gu, Wintergreen, Wuyi Grass, etc. Excavated in summer and autumn. Shanzi mushroom is sweet in taste, slightly pungent, and cool in nature. It has the effects of clearing heat and detoxifying, eliminating carbuncle and dissipating stagnation, etc. It is commonly used for carbuncle swollen furunculosis, scrofula with phlegm nucleus, snake bites, lame lumps and lumps.

[0003] At present, there are mainly three methods for artific...