A primer set and kit for detecting genes related to hypertension drug metabolism

A primer set and high blood pressure technology, applied in the fields of medicine and biology, can solve the problems of cumbersome operation, long detection cycle, uncontrollable factors of test results, etc., achieve simple sample processing, high sensitivity and specificity, and reduce adverse drug reactions Effect

Active Publication Date: 2021-12-07
PRECEDO PHARMA CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many methods for detecting gene mutations, the most commonly used are polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP method), sequence-specific PCR, manual or automatic sequencing, gene chip (gene chip, Also known as DNA chips), these methods are cumbersome to operate, the detection cycle is long, and the factors affecting the test results are uncontrollable, so they are currently only used for scientific research

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A primer set and kit for detecting genes related to hypertension drug metabolism
  • A primer set and kit for detecting genes related to hypertension drug metabolism
  • A primer set and kit for detecting genes related to hypertension drug metabolism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] Embodiment 1: the preparation of kit

[0098] 1. Design and synthesis of primers for genes related to hypertension drug metabolism

[0099] Using oligo software to design BDKRB2 gene, CYP11B2 gene, OAT1 / OAT3 gene, AGT gene, KCNH2 gene, LDLR gene, APOB gene, CACNA1C gene, GRK4 gene, ACE gene, CYP2D6 gene, CYP2C9 gene, CYP2C19 gene, ACE2 gene, AGTR1 Gene, ADRB2 gene and NR3C2 gene, the PCR amplification primers and sequencing primers of 17 hypertensive drug metabolism-related genes (see Table 1). The primer design follows the following principles: the length of the primer is about 25-30bp; the primer has no dimer; no hairpin structure; the Tm value difference between the upstream and downstream primers should not exceed 3°C; the GC content is about 50%. After the upstream and downstream primers are determined, use Blast to compare and analyze the upstream and downstream primers in the NCBI database to ensure the specificity and amplification efficiency of the primers; ...

Embodiment 2

[0106] Embodiment 2: the use of kit

[0107] 1. Sample testing

[0108] Take out the relevant reagents, and prepare the corresponding system according to the amount of template as follows: Take the PCR reaction solution, mix it evenly, add sample DNA, negative control substance or positive control substance as template to form a reaction system. Perform PCR amplification according to the PCR reaction procedure.

[0109] The main components of each gene detection system for hypertension are shown in Table 3.

[0110] Table 3. Main components of each gene detection system for hypertension

[0111]

[0112] The PCR reaction program of this system is shown in Table 4.

[0113] Table 4. PCR reaction program

[0114]

[0115] After the amplification is completed, agarose gel electrophoresis is performed to check the PCR amplification product, and the PCR amplification product is purified before proceeding to the next step.

[0116] 2. Sanger sequencing

[0117] Follow ...

Embodiment 3

[0133] Example 3: The effect of the primer set and / or kit of the present invention on the medication guidance of hypertensive patients

[0134] In order to verify the effect of the primer set and / or kit of the present invention on the drug guidance effect of hypertensive patients, volunteers with hypertension were selected at the "Present Medical Laboratory", and the genotype was performed using the invented primer set and / or kit. Test, according to the test results of each volunteer, accurately formulate its medication plan, and conduct clinical follow-up to observe the clinical drug effect.

[0135] Medication Guidance Example 1

[0136] The tester Tian, ​​who took spironolactone before the test, did not have a good effect. Using the primer set and / or kit of the present invention, Tian was tested for genes related to hypertension. According to Tian's gene detection results, he predicted hydrochlorothiazide and Propranolol is more effective. After Tian took propranolol ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a primer set for detecting genes related to hypertension drug metabolism based on Sanger sequencing method and a kit containing the primer set. The primer set of the present invention is characterized in that it includes primers for detecting at least one gene selected from the following genes: BDKRB2 gene, CYP11B2 gene, OAT1 / OAT3 gene, AGT gene, KCNH2 gene, LDLR gene, APOB gene, CACNA1C gene, GRK4 gene, ACE gene, CYP2D6 gene, CYP2C9 gene, CYP2C19 gene, ACE2 gene, AGTR1 gene, ADRB2 gene, and NR3C2 gene.

Description

technical field [0001] The invention relates to the fields of medicine and biotechnology, in particular to a primer set for detecting genes related to hypertension drug metabolism based on Sanger sequencing method, a kit using the primer set, and the use of the primer set in preparing the kit. Background technique [0002] Hypertension is a worldwide problem, and it cannot be cured at present. According to statistics, about 1 / 3 of the adults in the world suffer from high blood pressure. Hypertensive patients are prone to heart disease, stroke, chronic kidney disease and premature death, which pose a serious threat to human health and life. At present, the number of hypertensive patients in my country has reached 330 million, and the incidence rate is increasing year by year; drugs are the main means of treating hypertension and its complications at present and for a long time in the future. Currently, the five major classes of antihypertensive drugs are calcium channel bloc...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156
Inventor 刘娟周玲燕张定芳
Owner PRECEDO PHARMA CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap