Synthesis of silicon nanoparticle/gold nano-cluster ratio fluorescence probe and application thereof to fluorescence detection of rifampicin ratio
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A ratiometric fluorescent probe and silicon nanoparticle technology, applied in the field of fluorescent probes, can solve problems such as interference
Active Publication Date: 2018-12-28
JILIN INST OF CHEM TECH
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Problems solved by technology
At present, during the detection process of nano-fluorescent probes, it is usually based on a single fluorescence intensity change. This method is susceptible to interference from detection instruments and test environments.
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example 1
[0055] APTES in different amounts of substances was mixed with 12 mL of water to obtain an APTES solution. Add 30 μL of AA with a substance concentration of 100 mM, so that the molar ratios of AA and APTES are 1:50, 1:75, 1:100, and 1:150, respectively, and stir for 10 min; add 20 mL of Teflon In a dragon-lined reactor, hydrothermal reaction was carried out at 240 °C for 3 h to obtain SiNPs fluorescent probes.
[0056] figure 1 It is a bar graph of the effect of the volume ratio of AA to APTES solution on the fluorescence intensity of SiNPs. Depend on figure 1 It can be seen that when the molar ratio of AA to APTES is 1:100, the fluorescence intensity of the SiNPs fluorescent probe is the highest.
example 2
[0058] HAuCl at different volume concentrations of 10 mM 4 ·3H 2 O was mixed with 10 mL of water to obtain HAuCl 4 ·3H 2 O solution, add different amounts of MUA and 100 μL of 1 M NaOH to make HAuCl 4 ·3H 2 The molar ratios of O and MUA were 1:4, 1:5, 1:6, 1:7, and 1:8, respectively, and stirred for 5 h to obtain AuNCs fluorescent probes.
[0059] figure 2 for HAuCl 4 ·3H 2 The histogram of the effect of the volume ratio of O to MUA solution on the fluorescence intensity of AuNCs, given by figure 1 It can be seen that when HAuCl 4 ·3H 2 When the molar ratio of O to MUA solution was 1:6, the fluorescence intensity of AuNCs was the highest.
example 3
[0061] Add the solid-phase SiNPs to the HEPES buffer solution (pH ~ 7.4) to make the mass concentration 4 mg / mL, then add the solid-phase AuNCs to make the mass concentration 20 μg / mL, mix well and incubate for 0.5 h , to obtain SiNPs / AuNCs ratio fluorescent probes.
[0062] image 3 The emission fluorescence spectrum of the SiNPs / AuNCs ratio fluorescent probe. Depend on image 3 It can be seen that under the excitation spectrum with a wavelength of 300nm, the emission spectrum peaks of the SiNPs / AuNCs ratiometric fluorescent probes are at ~440 nm and ~615 nm, respectively.
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Abstract
The invention provides a synthesis method of a silicon nanoparticle/gold nano-cluster ratio fluorescence probe. The synthesis method comprises the following steps: firstly, preparing amino protected silicon nanoparticles by adopting a hydrothermal reduction method; then preparing a carboxyl protected gold nano-cluster by adopting a stirring reduction method; finally, carrying out self-assembly onthe silicon nanoparticles and the gold nano-cluster and synthesizing the ratio fluorescence probe. The synthesis method of the silicon nanoparticle/gold nano-cluster ratio fluorescence probe self-assembled at room temperature, provided by the invention, has the advantages of simple process, easiness in operation, mild conditions and short consumed time. The silicon nanoparticle/gold nano-cluster ratio fluorescence probe, provided by the invention, can accurately detect rifampicin by adopting a ratio fluorescence method; when the silicon nanoparticle/gold nano-cluster ratio fluorescence probe is applied to detect trace rifampicin in human serum; the result shows that the recovery rate of the silicon nanoparticle/gold nano-cluster ratio fluorescence probe is 97.0 to 103.5 percent and the relative standard deviation (RSDs) is lower than 4 percent.
Description
technical field [0001] The invention relates to the technical field of fluorescent probes, in particular to the synthesis of a silicon nanoparticle / gold nanocluster ratio fluorescent probe and its application to rifampicin ratio fluorescence detection. Background technique [0002] Rifampicin (RIF), also known as rifamycin, mepirifamycin, mepirifamycin, Weifuxian, Xiandaolun, Lifuping or rimidine. The chemical name is 3-[[(4-methyl-1-piperazinyl)imino]methyl]-rifamycin. RIF is a broad-spectrum antibiotic drug belonging to the rifamycin family, which has a strong antibacterial effect on Mycobacterium tuberculosis. RIF firmly binds to the β subunit of DNA-dependent RNA polymerase, inhibits the synthesis of bacterial RNA, prevents the enzyme from connecting with DNA, thereby blocking the RNA transcription process and stopping the synthesis of DNA and protein. The antibiotic has obvious bactericidal effect on Mycobacterium tuberculosis and some non-tuberculous mycobacteria (in...
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