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Lymphocyte irradiation damage SERS detection method

A technology of lymphocytes and detection methods, which is applied in the field of radiation biological dose estimation, can solve problems such as adverse effects and nanoparticle failure detection, and achieve the effects of overcoming high preparation costs, overcoming nanoparticle failure problems, and overcoming complicated steps

Active Publication Date: 2019-01-01
NAT UNIV OF DEFENSE TECH
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Problems solved by technology

However, due to problems such as sedimentation and agglomeration of nanoparticles, the nanoparticles will fail and have adverse effects on detection.

Method used

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  • Lymphocyte irradiation damage SERS detection method
  • Lymphocyte irradiation damage SERS detection method
  • Lymphocyte irradiation damage SERS detection method

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Embodiment Construction

[0021] The specific implementation manners of the embodiments of the present invention will be further described in detail below in conjunction with the drawings and embodiments. The following examples are used to illustrate the embodiments of the present invention, but are not intended to limit the scope of the embodiments of the present invention.

[0022] An embodiment of the present invention provides a SERS detection method for radiation-damaged lymphocytes. see figure 1 , the method includes: 101. Passing gold-shell magnetic beads, lymphocytes, and SERS tags into the microfluidic chip, stopping sample injection when the microfluidic chip is filled with solution, and an incubation reaction occurs, so that the gold-shell magnetic beads capture lymph The cells are further coupled to the SERS label; 102. A magnet is placed outside the detection area of ​​the microfluidic chip, and after the gold-shell magnetic beads are fully enriched under the magnetic field of the magnet,...

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Abstract

The embodiment of the invention provides a lymphocyte irradiation damage SERS detection method and belongs to the technical field of irradiation biological dose estimation. The method comprises the following steps that gold shell magnetic balls, lymphocytes and an SERS label are introduced into a micro-fluidic chip, and sample introduction is stopped and incubation reaction is generated after themicro-fluidic chip is fully filled with a solution, so that the gold shell magnetic balls capture the lymphocytes and thus generate coupling with the SERS label; and a magnet is arranged at the external of a detection region of the micro-fluidic chip, and the micro-fluidic chip is put on a portable raman spectrometer for raman detection after the gold shell magnetic balls are all enriched under amagnetic field of the magnet. According to the method, by preparing the micro-fluidic chip based on a gold shell magnetic ball raman substrate, the micro-fluidic chip has relatively high SERS performance compared with the traditional micro-fluidic chip, has the advantages of detection integration, automation, miniaturization and the like and overcomes the problems of complexity in step, relativelylarge interference with human factors, incapability in portability, efficacy losing of nano particles and the like of the traditional detection method.

Description

technical field [0001] Embodiments of the present invention relate to the technical field of radiation biological dose estimation, and more specifically, to a SERS detection method for radiation-damaged lymphocytes. Background technique [0002] Ionizing radiation will cause the phosphorylation of histone H2AX to γ-H2AX in human lymphocytes, and the expression level of γ-H2AX in human peripheral blood lymphocytes can be used as a characterization of the degree of radiation damage. At present, flow cytometry and fluorescence detection techniques are mainly used for the detection of lymphocyte γ-H2AX. Flow cytometry has the advantages of high detection accuracy and strong specificity, but due to factors such as high cost and high professionalism of operation, it is not feasible. It is conducive to the large-scale promotion of this technology. Fluorescence detection technology is widely used in the detection of γ-H2AX. It has mature technology, strong specificity, and high sen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 董培涛吴学忠王朝光张晨煜王京李白泥
Owner NAT UNIV OF DEFENSE TECH
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