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Detection method of radiation-damaged sers in lymphocytes

A lymphocyte and detection method technology, applied in the field of radiation biological dose estimation, can solve problems such as adverse effects and nanoparticle failure detection, and achieve the effects of overcoming high preparation costs, overcoming the problem of nanoparticle failure, and high SERS performance.

Active Publication Date: 2021-08-27
NAT UNIV OF DEFENSE TECH
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Problems solved by technology

However, due to problems such as sedimentation and agglomeration of nanoparticles, the nanoparticles will fail and have adverse effects on detection.

Method used

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  • Detection method of radiation-damaged sers in lymphocytes
  • Detection method of radiation-damaged sers in lymphocytes
  • Detection method of radiation-damaged sers in lymphocytes

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Embodiment Construction

[0021] The specific implementation manners of the embodiments of the present invention will be further described in detail below in conjunction with the drawings and embodiments. The following examples are used to illustrate the embodiments of the present invention, but are not intended to limit the scope of the embodiments of the present invention.

[0022] An embodiment of the present invention provides a SERS detection method for radiation-damaged lymphocytes. see figure 1 , the method includes: 101. Passing gold-shell magnetic beads, lymphocytes, and SERS tags into the microfluidic chip, stopping sample injection when the microfluidic chip is filled with solution, and an incubation reaction occurs, so that the gold-shell magnetic beads capture lymph The cells are further coupled to the SERS label; 102. A magnet is placed outside the detection area of ​​the microfluidic chip, and after the gold-shell magnetic beads are fully enriched under the magnetic field of the magnet,...

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Abstract

An embodiment of the present invention provides a SERS detection method for lymphocyte radiation damage, which belongs to the technical field of radiation biological dose estimation. The method includes: passing gold-shell magnetic beads, lymphocytes, and SERS tags into the microfluidic chip, stopping sample injection and incubating reaction when the microfluidic chip is filled with solution, so that the gold-shell magnetic beads capture the lymphocytes and then interact with the microfluidic chip. The SERS label is coupled; a magnet is placed outside the detection area of ​​the microfluidic chip, and after the gold-shell magnetic beads are fully enriched under the magnetic field of the magnet, the microfluidic chip is placed on a portable Raman spectrometer, and the Raman detection. By preparing a microfluidic chip based on a gold-shell magnetic bead Raman substrate, it has higher SERS performance than traditional microfluidic chips, and has the advantages of detection integration, automation, and miniaturization, which overcomes the steps of traditional detection methods. Complicated, highly disturbed by human factors, not portable, nanoparticle failure and other issues.

Description

technical field [0001] Embodiments of the present invention relate to the technical field of radiation biological dose estimation, and more specifically, to a SERS detection method for radiation-damaged lymphocytes. Background technique [0002] Ionizing radiation will cause the phosphorylation of histone H2AX to γ-H2AX in human lymphocytes, and the expression level of γ-H2AX in human peripheral blood lymphocytes can be used as a characterization of the degree of radiation damage. At present, flow cytometry and fluorescence detection techniques are mainly used for the detection of lymphocyte γ-H2AX. Flow cytometry has the advantages of high detection accuracy and strong specificity, but due to factors such as high cost and high professionalism of operation, it is not feasible. It is conducive to the large-scale promotion of this technology. Fluorescence detection technology is widely used in the detection of γ-H2AX. It has mature technology, strong specificity, and high sen...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 董培涛吴学忠王朝光张晨煜王京李白泥
Owner NAT UNIV OF DEFENSE TECH
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