Aspergillus coronatus loop-mediated isothermal amplification detection kit and detection method thereof

Abstract

The invention relates to a biological detection reagent, in particular to an Aspergillus coronatus loop-mediated isothermal amplification detection kit and a detection method thereof. According to thegenome sequence information of Aspergillus coronatus in the NCBI gene bank, the genome of aspergillus coronatus was compared with that of non-aspergillus coronatus, A set of specific primers for detecting LAMP of Aspergillus coronarius was designed according to the unique gene sequence of Aspergillus coronarius. Based on the primers, a set of LAMP detection method was established. The method hasthe characteristics of high specificity, high sensitivity, low cost, fast and easy operation.

Description

technical field [0001] The invention relates to a biological detection kit, in particular to a detection kit and a detection method for the isothermal amplification mediated by the coronoid sacrum ring. Background technique [0002] Fuzhuan brick tea grows into "golden flowers" through the "flowering" process under specific temperature and humidity conditions. Fuzhuan brick tea is a unique tea variety in China. There are few studies on "Golden Flower" abroad, and domestic research is relatively lagging behind. Based on the growth and morphological characteristics of the fungus, different researchers named it Aspergillus chevalieri), Aspergillus cristatum, Aspergillus spiculosus, and A. chevalieri var. intermidius of the Aspergillus gray-green group; until later, scanning electron microscopy was used to observe the ascus in detail The submicroscopic structure of spores and conidia is used to identify them. In "Mycology of China—Volume V: Aspergillus and related sexual types"...

AI Technical Summary

Problems solved by technology

National standards require the detection of S. coronoidis, but the traditional classification is cumbersome and requires expensive instruments such as scanning electron microscopes. Ordinary inspection and testing institutions cannot meet the requirements for accurate detection and identification; emerging DNA identification technologies can quickly and accurately identify microorganisms. The common ones are Ordinary PCR method and fluorescent quantitative PCR method (Li Hong et al., 2015; Xie Ronghui et al., 2017). The ordinary PCR method requires a PCR instrument and a gel imaging system, while the fluorescent quantitative PCR method requires an expensive fluorescent quantitative PCR instrument, grass-roots testing institutions and the general public. It is difficult for food processing enterprises to popularize the detection method

The loop-mediated isothermal amplification technique does not require expensive equipment and can detect target organisms accurately and sensitively (Zhou Zanhu et al., 2014). At present, there is no report on the detection of S. coronis by the loop-mediated isothermal amplification technique (Wang Zhigang et al., 1992; Chen Yongqiang et al., 2014)

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