Preserving fluid for micromolecule antigen and alkaline phosphatase labeled conjugate, and preparation method thereof

A preservation solution and small molecule technology, which is applied in the direction of analytical materials, measuring devices, instruments, etc., can solve the problems of loss of activity and inability to effectively maintain the activity of small molecule antigens and alkaline phosphatase

Active Publication Date: 2019-01-15
GUANGDONG FAPON BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the field of clinical application of chemiluminescent reagents, the use of competitive methods to detect small molecule antigens such as thyroxine (T4) and triiodothyronine (T3) often requires the combination of small molecule antigens with alkaline phosphatase markers, and small molecule antigens Molecular antigens and alkaline phosphatase-labeled conjugates are easily inactivated after they are prepared in common buffers, and the commonly used enzyme conjugate preservation solutions cannot effectively preserve the conjugates of small molecule antigens and alkaline phosphatase (ALP) Activity

Method used

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  • Preserving fluid for micromolecule antigen and alkaline phosphatase labeled conjugate, and preparation method thereof
  • Preserving fluid for micromolecule antigen and alkaline phosphatase labeled conjugate, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Preservation solution for small molecule antigen and alkaline phosphatase-labeled conjugate, its components are, based on the volume of the preservation solution, the components and their contents are as follows: HEPES 0.05mol / L, BSA 10g / L, mouse IgG 5g / L, sodium chloride 9g / L, magnesium chloride 1mmol / L, zinc chloride 0.1mmol / L, sucrose 10g / L, Tween 40 1g / L, Brij 35 1g / L, glycerol 50g / L, dextran 200000 10g / L, polyethylene glycol 4000 10g / L, ProClin 300 1g / L.

[0069] The preparation steps are as follows:

[0070] Prepare a clean container, add the following substances in 800ml distilled water in sequence:

[0071] Add HEPES as the buffer salt, stir to make it fully mixed, and adjust the pH to 7.0-7.5 with 1M HCl;

[0072] Add protein substance BSA and mouse IgG to the above solution, stir to make it fully mixed.

[0073] Add NaCl, MgCl 2 and ZnCl 2 , stir to mix thoroughly.

[0074] Add sucrose and stir to make it fully mixed.

[0075] Add surfactants Tween 40 a...

Embodiment 2

[0081] Preservation solution for small molecule antigen and alkaline phosphatase-labeled conjugate, its components are, based on the volume of the preservation solution, the components and their contents are as follows: HEPES 0.02mol / L, BSA 12g / L, mouse IgG 6g / L, sodium chloride 6g / L, magnesium chloride 5mmol / L, zinc chloride 1mmol / L, sucrose 50g / L, Tween 40 2.5g / L, Brij 35 2.5g / L, glycerol 80g / L, dextrose Sugar 200000 1g / L, polyethylene glycol 4000 1g / L, ProClin 300 0.8g / L.

[0082] The preparation steps are as follows:

[0083] Prepare a clean container, add the following substances in 800ml distilled water in sequence:

[0084] Add HEPES as the buffer salt, stir to make it fully mixed, and adjust the pH to 7.0-7.5 with 1M HCl;

[0085] Add protein substance BSA and mouse IgG to the above solution, stir to make it fully mixed.

[0086] Add NaCl, MgCl 2 and ZnCl 2 , stir to mix thoroughly.

[0087] Add sucrose and stir to make it fully mixed.

[0088] Add surfactants T...

Embodiment 3

[0094] Preservation solution for small molecule antigen and alkaline phosphatase-labeled conjugate, its components are, based on the volume of the preservation solution, the components and their contents are as follows: HEPES 0.05mol / L, BSA 7g / L, mouse IgG 3.5g / L, sodium chloride 15g / L, magnesium chloride 0.1mmol / L, zinc chloride 0.01mmol / L, sucrose 5g / L, Tween 40 0.25g / L, Brij 35 0.25g / L, glycerin 250g / L , Dextran 2000007.5g / L, polyethylene glycol 4000 7.5g / L, ProClin 300 2g / L.

[0095] The preparation steps are as follows:

[0096] Prepare a clean container, add the following substances in 800ml distilled water in sequence:

[0097] Add HEPES as the buffer salt, stir to make it fully mixed, and adjust the pH to 7.0-7.5 with 1M HCl;

[0098] Add protein substance BSA and mouse IgG to the above solution, stir to make it fully mixed.

[0099] Add NaCl, MgCl 2 and ZnCl 2 , stir to mix thoroughly.

[0100] Add sucrose and stir to make it fully mixed.

[0101] Add surfactan...

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Abstract

The invention relates to the field of in vitro diagnosis, and in particular to preserving fluid for a micromolecule antigen and an alkaline phosphatase labeled conjugate, and a preparation method thereof. According to the preserving fluid for the micromolecule antigen and the alkaline phosphatase labeled conjugate, the solvent of the preserving fluid is water, and counted by the volume of the preserving fluid, the preserving fluid comprises the following components: 0.2-0.05mol/L of buffer salt, 3-30g/L of protein substance, 0.1-0.4mol/L of saline ions, 5-50g/L of carbohydrate, 0.5-5g/L of surfactant, 50-270g/L of stabilizer and 0.5-5 g/L of preservative; and the pH of the preserving fluid is 7.0-8.0. The activity of thyroxine (T4), triiodothyronine (T3) and the alkaline phosphatase labeled conjugate in the preserving fluid after storage at 37 DEG C for 7 days can be effectively stabilized.

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to a preservation solution for a small molecule antigen and alkaline phosphatase labeled conjugate and a preparation method thereof. Background technique [0002] At present, the commonly used immunoassay method in the field of in vitro diagnosis is mainly chemiluminescence immunoassay (CLIA), which has been widely used in the clinical practice of virology, endocrinology, oncology, reproductive physiology, hematology, genetics and other medical and biological subjects. and research work. Among them, the labeled conjugate of alkaline phosphatase is needed in the enzyme-catalyzed chemiluminescent immunoassay, and the high sensitivity and good stability of the labeled conjugate of alkaline phosphatase are necessary guarantees for high-quality chemiluminescence analysis reagents. [0003] In the field of clinical application of chemiluminescent reagents, the use of competitive methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531
CPCG01N33/531
Inventor 郝明赵存洋夏良雨
Owner GUANGDONG FAPON BIOTECH CO LTD
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