Outside-bottle rapid rooting method for Dendrobium nobile Lindl

A Dendrobium nobile high-speed technology, applied in the field of seedling cultivation, can solve the problems of high cost of tissue culture technology, low planting survival rate, and high technical difficulty, and achieve the effect of low technical difficulty, high planting survival rate, and improved reproduction coefficient

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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of method that Dendrobium nobile nobile takes root rapidly outside the bottle, to solve the problem of the low reproduction coefficient of the current sub-pasting stone method and the tree-pasting method propagation method, the variety is easy to degenerate, and there are many diseases and insect pests; the cost of tissue culture technology is relatively high , the technical difficulty is relatively large, and the planting survival rate is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] (1) Seedling collection: select the stem tip growth point of the Dendrobium nobile plant as an explant, cut into sections, sterilize, inoculate on WPM medium, and induce clustered buds;

[0011] (2) Seedling pretreatment: After 40 days of induction culture, the Dendrobium explants grow new shoots on the induction medium, and then adjust the concentration of hormones in the medium to reduce to 1 / 2 of the original, and carry out subculture. Subculture once every 48 days, and carry out 6 generations of subculture to obtain initial tissue culture seedlings;

[0012] (3) Cultivation of strong seedlings: Under the condition of temperature of 15 degrees Celsius, carry out strong seedling cultivation on the above initial tissue cultured seedlings, and when the height of the tissue cultured seedlings reaches 5 cm, the strong seedlings will be obtained;

[0013] (4) Cuttings of tissue culture seedlings: hang the plants on the planting board, insert part of the root system into th...

Embodiment 2

[0018] (1) Seedling collection: select the stem tip growth point of the Dendrobium nobile plant as an explant, cut into sections, sterilize, inoculate on WPM medium, and induce clustered buds;

[0019] (2) Seedling pretreatment: After 50 days of induction culture, the Dendrobium explants grow new shoots on the induction medium, and then adjust the concentration of hormones in the medium to reduce to 1 / 2 of the original, and carry out subculture. Subculture once every 52 days, carry out subculture for 7 generations, and obtain initial tissue culture seedlings;

[0020] (3) Cultivation of strong seedlings: Under the condition of temperature of 30 degrees Celsius, carry out strong seedling culture on the above initial tissue cultured seedlings, and when the height of the tissue cultured seedlings reaches 8 cm, the strong seedlings will be obtained;

[0021] (4) Cuttings of tissue culture seedlings: hang the plants on the planting board, insert part of the root system into the nut...

Embodiment 3

[0026] (1) Seedling collection: select the stem tip growth point of the Dendrobium nobile plant as an explant, cut into sections, sterilize, inoculate on WPM medium, and induce clustered buds;

[0027] (2) Seedling pretreatment: After 45 days of induction culture, the Dendrobium explants grow new shoots on the induction medium, and then adjust the concentration of hormones in the medium to reduce to 1 / 2 of the original, and carry out subculture. Subculture once every 50 days, and carry out subculture for 7 generations to obtain initial tissue culture seedlings;

[0028] (3) Cultivation of strong seedlings: Under the condition of temperature of 20 degrees Celsius, carry out strong seedling cultivation on the above initial tissue cultured seedlings, and when the height of the tissue cultured seedlings reaches 7 cm, the strong seedlings will be obtained;

[0029] (4) Cuttings of tissue culture seedlings: hang the plants on the planting board, insert part of the root system into t...

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Abstract

The invention relates to an outside-bottle rapid rooting method for Dendrobium nobile Lindl, wherein the outside-bottle rapid rooting method comprises: (1) seedling collection; (2) seedling pretreatment; (3) strong seedling culture; (4) tissue culture seedling cutting; and (5) post-cutting management. According to the present invention, the Dendrobium nobile Lindl seedling is pre-treated, the hydroponic seedling culture method is used, the Dendrobium nobile Lindl seedling rooting is induced by using the WPM culture medium through the indoor constant temperature culture, and the Dendrobium nobile Lindl seedling is transplanted to the field planting plate and is cultured to form the commercial acclimation seedling after two months of the germination of the Dendrobium nobile Lindl seedling, such that the breeding coefficient is increased, and the variety degradation is avoided; the Dendrobium nobile Lindl seedling is treated with the insect-repellent liquid so as to reduce plant diseasesand insect pests; and the common equipment is used, such that the cost of the tissue culture technology is low, the technical difficulty is low, and the survival rate of planting is high.

Description

technical field [0001] The invention relates to the technical field of seedling cultivation, in particular to rooting and seedling cultivation outside a bottle, in particular to a rapid rooting method for Dendrobium nobile off a bottle. Background technique [0002] Dendrobium nobile, also known as: Wanzhangxu, Nobile Stone, Flat Nobile, Flat Yellow Grass, Flat Grass, is a perennial herb, named for its shape like the hairpin on the ancient head. The stems are clustered, the upper part is slightly flattened and slightly curved, 10-60 cm high and 1.4 cm thick. It likes to grow in a warm, humid, half shade and half sun environment. The origin of Dendrobium is mainly distributed in tropical and subtropical Asia, Australia and Pacific Islands. There are more than 1,000 species of Dendrobium in the world. There are about 76 species in China, most of which are distributed in Southwest China, South China, Taiwan and other places. Dendrobium is the largest genus in the orchid fami...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G2/10A01G13/00
CPCA01H4/001A01G2/10A01G13/00A01H4/008
Inventor 陈风雷孟凡平余胜梅
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