Flammulina velutipes liquid strain culture solution and preparation method thereof
A technology of liquid strains and culture fluid, applied in botany equipment and methods, medium, cultivation, etc., can solve problems such as toxic side effects, poor effect, instability, etc., and achieve increased lysine and arginine content, The effect of avoiding space pollution and increasing the adsorption capacity
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[0039] On the other hand, the embodiment of the present invention also provides a preparation method of Flammulina velutipes liquid strain culture solution, comprising the following steps:
[0040] Step a: Take half of the weight of soybean meal, silkworm chrysalis, yeast and lichen powder respectively, and add them in turn to a constant temperature oven, and continue drying in a high temperature environment of 80-85°C for 45-60min, and then dry the dried soybean meal , silkworm chrysalis, yeast and lichen are crushed and sieved to obtain respectively soybean meal powder, silkworm chrysalis powder, yeast powder and lichen powder for subsequent use;
[0041] Step b: Cut the ripe tomatoes and apples into pieces, put them into a fruit juice machine and grind them thoroughly, then filter out the filter residue, put them into centrifuge cups and centrifuge for 10-15min in a centrifuge, and finally take the supernatant Liquid, obtain tomato extract and apple extract for subsequent u...
Embodiment 1
[0063] The liquid culture medium that present embodiment improves the glutamic acid content of Flammulina velutipes is prepared from the following components by weight: 3 parts of soybean meal powder, 3 parts of silkworm chrysalis powder, 0.5 part of yeast powder, 0.5 part of lichen powder, 3 parts of glucose, 3 parts of trehalose, 2 parts of monosodium glutamate, 1 part of tomato extract, 1.2 parts of apple extract, 0.5 parts of paclobutrazol powder, 0.4 parts of anti-fall powder, 3 parts of chitosan, 0.5 parts of magnesium carbonate, 20 parts of trehalose, yeast 2 parts of powder, 1 part of peptone, 1 part of potassium dihydrogen phosphate, 0.1 part of graphene oxide, 0.1 part of N-butylpyridine hexafluorophosphate, 0.1 part of sodium carboxymethyl cellulose, 0.2 part of polyacrylamide, 1.3 parts of nano calcium carbonate part, 0.2 part of sodium lauryl sulfonate, the balance is pure water, and the pH value of the culture medium is adjusted to 5-6.
Embodiment 2
[0065]In this embodiment, the liquid culture medium for improving the glutamic acid content of Flammulina velutipes is prepared from the following components by weight: 2-3 parts of soybean meal powder, 2-3 parts of silkworm chrysalis powder, 0.3-0.5 part of yeast powder, and 0.1 part of lichen powder. -0.5 parts, 1.5-3 parts of glucose, 1-3 parts of trehalose, 1-2 parts of monosodium glutamate, 0.5-1 parts of tomato extract, 0.7-1.2 parts of apple extract, 0.3-0.5 parts of paclobutrazol powder and anti-falling powder 0.2-0.4 parts, the balance is water, 5 parts of chitosan, 2.5 parts of magnesium carbonate, 25 parts of trehalose, 6 parts of yeast powder, 2 parts of peptone, 2 parts of potassium dihydrogen phosphate, 1.5 parts of graphene oxide, N- 0.85 parts of butylpyridine hexafluorophosphate, 0.5 parts of sodium carboxymethylcellulose, 0.8 parts of polyacrylamide, 9.5 parts of nano-calcium carbonate, 0.8 parts of sodium lauryl sulfonate, the balance is pure water, and the p...
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