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Method for establishing in-tube leaf-cuttage rapid-propagation system of petrocosmea qinlingensis

An establishment method, a technology in test tube, applied in the field of plant tissue culture, can solve the problems of slow propagation mode, rapid propagation of Qinling stone butterfly without establishment, no successful examples, etc., achieve technology easy to master, overcome the slow natural propagation mode, The effect of high seedling rate

Inactive Publication Date: 2019-01-29
SHAANXI SCI TECH UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The rapid propagation of Qinling stone butterfly by using plant tissue culture technology can obtain a large number of high-quality seedlings consistent with the genetic background of the mother plant in a short period of time, and overcome the characteristics of slow natural reproduction. However, no Qinling stone butterfly has been established before this. Reports of rapid reproduction of butterflies in test tubes, and no successful examples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Preparation of culture medium: first prepare the culture medium used in the Qinling stone butterfly cultivation process, including the basic medium and the specific medium with additional MS nutrient solution and hormones. The preparation process of the basic medium is as follows: 2:1 Measure perlite, vermiculite and peat at a volume ratio of 1 and add water to mix them evenly. After absorbing water and saturate, put them into 240mL culture bottles respectively. The filling volume is 1 / 3 of the culture bottle volume. Sterilize the culture bottle at 121.3°C for 60 minutes; the proliferation culture medium is MS nutrient solution + IBA, and the concentration of IBA is 100 mg / L; the rooting and strong seedling culture medium is MS nutrient solution + IBA, and the concentration of IBA is 200 mg / L; The MS nutrient solution used in the culture solution and the rooting and strong seedling culture solution does not add sucrose, and its pH value is 5.8.

[0024] (2) Disinfec...

Embodiment 2

[0028] The basic steps are the same as in Example 1, except that the proliferation culture medium used in step (3) is composed of 1 mL of MS nutrient solution and 1 mL of IBA with a concentration of 200 mg / L, and the pH is 5.8.

[0029] After adding this proliferation culture solution and cultivating under the same conditions as in Example 1, adventitious buds began to form in the 4th week, and adventitious buds could be formed on the front and back of the blade, most of which occurred at the base of the blade near the petiole, and a few occurred at the edge of the leaf. The multiplication coefficient of each leaf was 11.67 in the fifth week, and the maximum multiplication coefficient of a single leaf was 16. The average multiplication coefficient of each leaf was 51 until the eighth week, and the largest multiplication coefficient of a single leaf reached 68.

Embodiment 3

[0031] The basic steps are the same as in Example 1, except that the proliferation culture medium used in step (3) is composed of 1 mL of MS nutrient solution and 1 mL of IBA with a concentration of 400 mg / L, and the pH is 5.8.

[0032] After adding the proliferation culture solution and culturing under the same conditions as in Example 1, adventitious buds began to form at the base of the petiole of the blade in the 4th week, and a small amount of white hair-like roots were formed at the base of the petiole. The maximum multiplication coefficient of a single leaf was 9.33, and the maximum multiplication coefficient of a single leaf was 12. The average multiplication coefficient of each leaf was 33.67 until the eighth week of cultivation, and the maximum multiplication coefficient of a single leaf was 37.

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PUM

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Abstract

The invention discloses a method for establishing an in-tube leaf-cuttage rapid-propagation system of petrocosmea qinlingensis. The method comprises the following three steps: disinfecting and inoculating explant blades, carrying out leaf-cuttage proliferation culture and carrying out rooting and seedling growing culture, wherein a basic culture medium and a specific culture medium with an MS nutrient solution and hormone are used in the culture process. Firstly, the blades used as explants are subjected to wiping, vibration washing, washing, sterilization, and washing by deionized water, thenthe petiole end is obliquely inserted into the basic culture medium, a proliferation culture solution is added to the base part of the petiole to induce formation of adventitious buds, finally the formed adventitious buds are divided into single plants and are transferred into the basic culture medium, a rooting growing seedling culture solution is added from roots of single buds for carrying outrooting and seedling growing. According to the method disclosed by the invention, leaf-cuttage rapid-propagation culture of the petrocosmea qinlingensis is realized through a tissue culture technology, the propagation coefficient is high, the characteristic that a natural propagation mode is low can be overcome, and a large number of high-quality seedlings with stable and consistent genetic characters can be obtained in a short time, so that the method has positive significance for the rapid propagation and germplasm resource protection of the petrocosmea qinlingensis.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for establishing a rapid propagation system of Qinling stone butterfly in test tube leaf cuttings. Background technique [0002] Qinling Stone Butterfly (Petrocosmea qinlingensis W.T.Wang) is a plant of the Gesneriaceae (Gesneriaceae) Stone Butterfly (Petrocosmea) plant. It is a perennial herb unique to the Qinling Mountains. Its flowering period is from August to September. It is born on mountain rocks at an altitude of 650 meters. It is listed as a national second-class key protected wild plant and is designated as an endangered species. Qinling Stone Butterfly is the northernmost species of the genus Stone Butterfly, and its natural population is very small. Many field surveys have not found its wild population. Researchers once thought that this species was extinct. In 2015, the survey of wild plant resources in Shaanxi Province rediscovered this specie...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 胡选萍蒋景龙王琦李耘秦公伟胡凤成赵新峰
Owner SHAANXI SCI TECH UNIV
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