Cell culture method for increasing yield of polysaccharide of Niudali

A cell culture and powerful technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem that the output of wild schizophrenia can not meet the market demand.

Inactive Publication Date: 2019-02-12
梁伟艺
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The output of wild Spathiphyllum pl...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A cell culture method for improving bovine herbaceous polysaccharide production, comprising:

[0052] Step 1) First, select the soon-to-mature seeds of Spatholobus Spatholobus, then sterilize the immature seeds, and then treat them at a temperature of 0° C. for 24 hours, and finally place them in a germination liquid medium for germination and culture until they grow 1 mm-long shoots. leaves, the soon-to-be-ripe seeds are the seeds of the Millipede spatholobus plant 90 days after flowering, and the disinfection includes: first cleaning the soon-to-mature seeds several times with a solution containing a mass volume concentration of 1g / mL calcium hypochlorite, and then The washed seeds were treated at a temperature of 70° C. for 20 seconds. The germination liquid medium contained: 1 / 4MS, 7 g / L sucrose and 0.1 mg / L 6-BA, and its initial pH value was 5.8.

[0053] Step 2) select the tender leaves grown through germination and culture as explants, place the explants on the i...

Embodiment 2

[0067] A cell culture method for improving bovine herbaceous polysaccharide production, comprising:

[0068] Step 1) First, select the soon-to-mature seeds of Mildew Spatholobus, then sterilize the immature seeds, then treat them at a temperature of 2°C for 48 hours, and finally place them in a germination liquid medium for germination and culture until they grow 10mm-long shoots. leaves, the soon-to-be-ripe seeds are the seeds of the Millipede spathifolia plant 130 days after flowering, and the disinfection includes: firstly, using a solution containing mass volume concentration 5g / mL calcium hypochlorite to clean the soon-to-be-ripe seeds for several times, and then The washed seeds were treated at a temperature of 80° C. for 30 seconds. The germination liquid medium contained: 1 / 2 MS, 15 g / L sucrose and 0.3 mg / L 6-BA, and its initial pH value was 5.8.

[0069] Step 2) select the tender leaves grown through germination and culture as explants, place the explants on the induc...

Embodiment 3

[0083] A cell culture method for improving bovine herbaceous polysaccharide production, comprising:

[0084] Step 1) First, select the soon-to-mature seeds of Mildew Spatholobus, then sterilize the immature seeds, and then treat them at a temperature of 1°C for 36 hours, and finally place them in a germination liquid medium for germination and culture until they grow 5mm-long shoots. leaves, the soon-to-be-mature seeds are the seeds of the plant of Miltonia spaghetti 110 days after flowering, and the disinfection includes: firstly, using a solution containing mass volume concentration 3g / mL calcium hypochlorite to clean the soon-to-mature seeds for several times, and then The washed seeds were treated at a temperature of 75° C. for 25 seconds. The germination liquid medium contained: 1 / 3 MS, 11 g / L sucrose and 0.2 mg / L 6-BA, and its initial pH value was 5.8.

[0085]Step 2) select the tender leaves grown through germination and culture as explants, place the explants on the in...

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Abstract

The invention discloses a cell culture method for increasing the yield of polysaccharide of Niudali. The cell culture method comprises the following steps: (1) seeds to be matured of Caulis Spatholobiare selected, disinfected, treated, and placed into a germination liquid culture medium to be subjected to germination culture, wherein the seeds to be matured are seeds of Caulis Spatholobi plants in 90-130 days after flowering, and disinfection comprises the steps: the seeds are cleaned through a calcium hypochlorite solution and then treated for 20-30 seconds under the temperature of 70-80 DEGC; (2) tender leaves are taken as explants for induction culture, and a callus is obtained; (3) the callus is subcultured for two or more times till the callus grows into a cell cluster; (4) the cellcluster is subjected to suspension starting culture, and a suspension cell line is obtained; (5) the suspension cell line is subjected to enrichment culture to obtain a Caulis Spatholobi suspension cell line; and (6) cells of the Caulis Spatholobi suspension cell line are collected, extraction is conducted through pure water for three times, extracts of the three times are combined, centrifuge, precipitation and drying are conducted, and then the polysaccharide of Niudali is obtained.

Description

technical field [0001] The invention relates to the technical field of cultivation of traditional Chinese medicinal materials, in particular to a cell culture method for increasing the yield of bovine herbaceous polysaccharide. Background technique [0002] Spatholobus Spatholobus, the name of traditional Chinese medicine. It is the dried cane of Spatholobus suberectus Dunn, a leguminous plant. Harvest in autumn and winter, remove branches and leaves, slice and dry in the sun. Distributed in Guangdong, Guangxi, Yunnan and other places. It has the effects of invigorating blood and nourishing blood, regulating menstruation and relieving pain, relaxing tendons and activating collaterals. For irregular menstruation, dysmenorrhea, amenorrhea, rheumatic arthralgia, numbness and paralysis, blood deficiency and chlorosis. With the deepening of research, scientists have found that the roots of Mildew Spatholobus, Niu Dali are rich in polysaccharides and other substances, which ha...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 梁伟艺钟静海
Owner 梁伟艺
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