Plant living cell capture and control device and method based on optical fiber probe

Abstract

The invention discloses a plant living cell capture and control device and method based on an optical fiber probe. The plant living cell capture and control device based on the optical fiber probe comprises a laser, a computer, a charge coupling element, a microscope, an objective lens, the optical fiber probe, a glass tube, a glass slide, an optical fiber adjusting frame and an objective table. The plant living cell capture and control method based on the optical fiber probe comprises the following specific steps of S1, preparing the optical fiber probe for capture and control; S2, assemblinga one-dimensional chloroplast array; S3, controlling the one-dimensional chloroplast array to move in living cells; and S4, assembling a two-dimensional chloroplast array. The control, arrangement and assembly of typical organelle chloroplasts in plant cells are realized, which is of great importance for plant photosynthesis and gene engineering.

Description

technical field [0001] The invention belongs to the field of optical technology, in particular to a device for capturing and manipulating living plant cells based on an optical fiber probe and a method thereof. Background technique [0002] Traditional optical tweezers can capture and manipulate the cells on the surface of the living body without contact, but their penetration depth is limited, and they have complex, expensive devices and huge optical systems, so they cannot go deep into the living body to capture and manipulate deep cells. [0003] The existing in vivo manipulation method can realize the capture and manipulation of cells in vivo. It uses a laser to generate a gradient force on the cell or particle, and pulls the cell or particle to the focus of the field gradient force to complete the capture and manipulation, but it can only It is difficult to simultaneously manipulate, arrange and assemble multiple cells or even the organelles inside cells by capturing or...

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a plant living cell capture and manipulation device based on an optical fiber probe, which solves the problems of the limited penetration depth and huge optical system of traditional optical tweezers in the prior art

[0005] Another object of the present invention is to provide a method for capturing and manipulating plant living cells based on fiber optic probes, which solves the problem that traditional optical tweezers cannot capture and manipulate cells deep into the living body and it is difficult to simultaneously capture and control multiple cells or even cells in the prior art. The problem of manipulation, arrangement and assembly of organelles inside cells

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