Nucleotide molecule combination for SNP (single nucleotide polymorphism) detection of fluorouracil metabolism-related genes

Abstract

The invention discloses a group of nucleotide molecule combinations for SNP(single nucleotide polymorphism) detection of fluorouracil metabolism-related genes. The invention also provides an SNP fluorescence in-situ hybridization sequencing detection method for detecting fluorouracil and fluorouracil derivative drug metabolism-related genes GSTP1 and DPYD by the nucleotide molecule combinations. The method comprises the following steps: extracting DNA of a sample to be tested, performing single-stranded derivatization by taking the DNA as a template, then simultaneously adding a first sequencing probe and a second sequencing probe of different fluorescent labels to hybridize with a single-stranded derivative, and finally interpreting a hybridization result. The method is high in precision,good in stability, fast, safe and easy to operate automatically. By adopting the method, accurate classification of single nucleotide polymorphisms can be completed in the single-stranded derivatization and the hybridization reaction cycle, so that a genotype of a subject can be understood, and the prevention of diseases caused by risk factors and guidance for the use of drugs for clinical individuals can be achieved.

Description

technical field [0001] The invention discloses a single nucleotide polymorphism detection method, which belongs to the field of molecular genetics. Background technique [0002] With the continuous progress of the development of medical science, many drugs appear in the world every year, but they are eliminated in drug research because of too many adverse reactions. Although the effects and adverse reactions of drugs are related to race and individual differences, genetic differences in genome sequences between people are important reasons for drug efficacy and adverse reactions. Single Nucleotide Polymorphisms (Single Nucleotide Polymorphisms; SNP) is the difference between 1 base between people. There are several genome sequence differences, and SNP is only the most common and common one. Clinically Significant. [0003] SNP is a polymorphism in DNA sequence caused by a single base change. Including single base conversion, inversion and single base insertion or deletion...

AI Technical Summary

Problems solved by technology

[0012] (3) Adverse reactions between SNP and drugs: In the course of medical treatment, some patients will not only be ineffective after taking the drugs, but some will also have serious adverse reactions, such as improper handling and sometimes death

There are several problems in this type of technology: only one hybridization is performed, and it is difficult to identify non-specific signals, so some non-specific hybridization may cause sequence misreading. In addition, the cost of the chip is high, and the required equipment is expensive, which is not conducive to popularization and application.

This method is prone to errors when the fluorescence curves of homozygous genotypes and heterozygous genotypes are relatively close, and cannot give accurate results, which may easily cause false positives or misjudgments.

[0026] In view of the technical problems such as high misjudgment rate, poor accuracy, low stability, and low detection efficiency in the detection of single nucleotide polymorphisms in genes related to fluorouracil metabolism in the prior art, the present invention intends to provide an improvement on the prior art. To improve, by designing single-stranded derived guide sequences and probe sequences, optimizing each component, a set of nucleotide molecule combinations for SNP detection of fluorouracil metabolism-related genes is provided, and then the nucleotide molecules using the nucleotide molecules are provided SNP fluorescence in situ hybridization sequencing detection method for combined detection of fluorouracil and its derivatives drug metabolism-related genes

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