Nucleotide molecule combination for SNP (single nucleotide polymorphism) detection of fluorouracil metabolism-related genes

A technology of oligonucleotide molecules and fluorouracil, which is applied in the field of single nucleotide polymorphism detection, can solve the problems of high chip cost, death, high misjudgment rate, etc., and achieve fast sequencing speed, high recognition, and less pollution Effect
CN109337984AInactive Publication Date: 2019-02-15北京华夏时代基因科技发展有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
北京华夏时代基因科技发展有限公司
Publication Date
2019-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a group of nucleotide molecule combinations for SNP(single nucleotide polymorphism) detection of fluorouracil metabolism-related genes. The invention also provides an SNP fluorescence in-situ hybridization sequencing detection method for detecting fluorouracil and fluorouracil derivative drug metabolism-related genes GSTP1 and DPYD by the nucleotide molecule combinations. The method comprises the following steps: extracting DNA of a sample to be tested, performing single-stranded derivatization by taking the DNA as a template, then simultaneously adding a first sequencing probe and a second sequencing probe of different fluorescent labels to hybridize with a single-stranded derivative, and finally interpreting a hybridization result. The method is high in precision,good in stability, fast, safe and easy to operate automatically. By adopting the method, accurate classification of single nucleotide polymorphisms can be completed in the single-stranded derivatization and the hybridization reaction cycle, so that a genotype of a subject can be understood, and the prevention of diseases caused by risk factors and guidance for the use of drugs for clinical individuals can be achieved.
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Description

technical field

[0001] The invention discloses a single nucleotide polymorphism detection method, which belongs to the field of molecular genetics. Background technique

[0002] With the continuous progress of the development of medical science, many drugs appear in the world every year, but they are eliminated in drug research because of too many adverse reactions. Although the effects and adverse reactions of drugs are related to race and individual differences, genetic differences in genome sequences between people are important reasons for drug efficacy and adverse reactions. Single Nucleotide Polymorphisms (Single Nucleotide Polymorphisms; SNP) is the difference between 1 base between people. There are several genome sequence differences, and SNP is only the most common and common one. Clinically Significant.

[0003] SNP is a polymorphism in DNA sequence caused by a single base change. Including single base conversion, inversion and single base insertion or deletion...

Claims

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