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Biological detection system

A biological detection system and detection device technology, applied in the field of biological detection systems, can solve the problems of manual or semi-automatic detection process, high possibility of pollution, narrow dynamic range, etc., to improve sample utilization rate, avoid sample waste, and small temperature shock Effect

Active Publication Date: 2019-02-15
杭州莱约科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a biological detection system that solves the problems of insufficient sample volume, high possibility of contamination, narrow dynamic range, long detection time, manual operation of sample numbering, manual or semi-automatic detection process, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] When used as a general-purpose PCR amplification, the amplification conditions of the kit are as follows:

[0126] The set temperature of T0 is S0=98℃, and the length of the temperature zone is 5cm

[0127] T1, T4, T7...T88 set the temperature as S1 = 95°C, and the length of each temperature zone is 1cm

[0128] T2, T5, T8...T89 set the temperature as S2 = 60°C, and the length of each temperature zone is 1cm

[0129] T3, T6, T9...T90 set the temperature as S3 = 72°C, and the length of each temperature zone is 2cm

[0130] When the reaction vessel moves along the temperature zone at a speed of 1cm / min and conducts heat transfer with the temperature zone, the temperature of the temperature zone is transferred to the reaction vessel, and the reaction chamber of each reaction vessel has undergone 5min 98°C pre-denaturation, 30 A cycle of denaturation at 95°C for 60s, renaturation at 60°C for 60s, and extension at 72°C for 120s achieved a nucleic acid amplification.

Embodiment 2

[0132] When used as digital PCR amplification, the amplification conditions of the kit are as follows:

[0133] T0 set temperature as S0=98℃, length 5cm

[0134] T1, T3, T5, T7...T79 set the temperature as S1 = 95°C, and the length of each temperature zone is 1cm

[0135] T2, T4, T6, T8...T80 set temperature as S2=60℃, length 1cm

[0136] T81...T90 set temperature is ambient temperature.

[0137] When the reaction vessel moves along the temperature zone at a speed of 2 cm / min and conducts heat transfer with the temperature zone, the temperature of the temperature zone is transferred to the reaction vessel, and the reaction chamber of each reaction vessel undergoes 2.5min 98°C pre-denaturation, 40 One cycle of denaturation at 95°C for 30s and renaturation at 60°C for 30s achieved one nucleic acid amplification.

Embodiment 3

[0139] When incubating as a protein, the kit antibody incubation conditions are as follows:

[0140] The set temperature of T0 is S0=37℃, the length is 5cm

[0141] T1, T3, T5, T7...T55 set the temperature as S1 = 37°C, and the length of each temperature zone is 1cm

[0142] T56...T90 set temperature is ambient temperature.

[0143] When the reaction vessel moves along the temperature zone at a speed of 1cm / min and conducts heat transfer with the temperature zone, the temperature of the temperature zone is transferred to the reaction vessel, and the reaction chamber of each reaction vessel has been incubated for 60 minutes to complete the antibody incubation. combined.

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Abstract

The invention discloses a biological detection system. The biological detection system comprises: a reaction vessel with micropores thereon; a loading device which is used for filling a reaction system into the micropores of the reaction vessel; a temperature control device which is used for carrying out the temperature control on the reaction vessel filled with the reaction system so as to causethe reaction system in the reaction vessel to perform a chemical reaction or a biological reaction; and a detection device which is used for detecting and recognizing specific fluorescence signal characteristics exhibited by the reaction in the reaction vessel, recognizing or identifying chemical or / and biological signals in each micropore of the reaction vessel, analyzing signal features in the micropores, recognizing a negative proportion according to the poisson distribution principle, and calculating the concentration of a biological sample in the original reaction system. The system continuously loads, continuously processes, continuously detects and continuously analyzes the sample; by manners described above, the system is high in sensitivity, high in accuracy, good in specificity,and wide in dynamic range; the characteristics of less loading amount in the nucleic acid detection or the protein detection and narrow dynamic range is changed, and the dynamic range is greatly expanded.

Description

technical field [0001] The invention relates to the field of biological analysis and detection of biological detection, in particular to a biological detection system for quantitative detection of nucleic acid or protein. Background technique [0002] When detecting small molecules, nucleic acids, proteins, and bacteria, during nucleic acid extraction or purification of biological samples, the sample extraction volume is generally 50 μL to 200 μL, and the sample volume of existing instruments is generally several μL. The sample volume is small and cannot fully Using extracted samples, the dynamic range is narrow, especially for rare samples, which cannot be fully detected after extraction, but it is difficult to take into account the needs of rare samples and sample volume; existing devices are generally manual or semi-automatic, and the operation process is cumbersome, which is easy to cause Contamination and operational uncertainty; some of the existing equipment has uncer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6439
Inventor 杨和平夏敏青王珊
Owner 杭州莱约科技有限公司
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