Method for rapid identification of yak, cattle and dzo beef

Abstract

The invention belongs to the technical field of molecular biological identification methods and particularly relates to a method for rapid identification of yak, cattle and dzo beef. The method includes steps: acquiring a to-be-identified beef sample, extracting a DNA sample, designing specific primers, subjecting the to-be-identified beef sample to PCR amplification, adopting agarose gel for electrophoresis of a product obtained after amplification, and judging a type of the beef according to an electrophoresis result. The method which is a simple, convenient, quick, efficient and accurate beef type identification method is low in cost and has a promising application prospect.

Description

technical field [0001] The invention belongs to the technical field of molecular biological identification methods, and in particular relates to a method for rapidly identifying yaks, cattle, and beef. Background technique [0002] The current meat product market is mixed with fish and dragons, and the adulteration of meat products has brought great difficulties and requirements to the supervision of the meat product quality market. Meat adulteration, for example, uses pork as mutton or beef, and duck, chicken, and even mouse meat as more expensive beef and mutton. This is not only related to the health of consumers, but also related to religious beliefs, etc. The health of consumers from unknown sources is therefore facing a huge threat. [0003] Beef is the meat most consumers prefer, especially in Qinghai, Xinjiang, Tibet and other provinces and cities with large beef consumption. However, due to eating habits, people also have preferences for different beef. Because ya...

AI Technical Summary

Problems solved by technology

However, due to eating habits, people also have preferences for different beef. Because yaks mostly live in cold pastoral areas, and most of them have been grazed, their green and organic environment is favored by many consumers. The catering market The sales of yak meat are extremely hot, so some people cheat from it and use ordinary beef (such as yellow beef and P beef) as yak meat to deceive consumers

[0004] At present, common and effective meat identification methods include sensory testing for identification, such as: color, fiber thickness, etc. This identification method requires the identification personnel to have rich experience and has low accuracy; the other is through the identification of specific DNA fragments Amplification, recovery, enzyme digestion, electrophoresis detection and identification of the target band, the steps are slightly cumbersome

Images