Body restoration and pretreatment method before identification of zooplankton samples

A zooplankton and pretreatment technology, applied in the preparation of test samples, etc., can solve the problems that cannot be popularized and used, and the difference is far away, and achieve the effect of facilitating the recovery of the body

Active Publication Date: 2019-02-19
PEARL RIVER HYDRAULIC RES INST OF PEARL RIVER WATER RESOURCES COMMISSION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But above-mentioned patent all is to have developed new fixative, although have better effect, owing to differ very far with national standard method, thus can't popularize and use

Method used

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  • Body restoration and pretreatment method before identification of zooplankton samples
  • Body restoration and pretreatment method before identification of zooplankton samples
  • Body restoration and pretreatment method before identification of zooplankton samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 zooplankton sample pretreatment method

[0035] 1. Fixed body recovery solution, including three parts:

[0036] (1) The collected zooplankton copepods (the collection location is the Wuzhou section of Xijiang River, using 13# zooplankton net to collect more) samples, the fixative adopts the formaldehyde that adds 5% of the sample volume, affixes a label, and puts it in a cool place Preserve between dry samples; after one year of preservation, filter the zooplankton copepod samples with a 13# net or a sieve of equal aperture to separate the zooplankton from the fixative solution (formaldehyde solution);

[0037] (2) Add Tris-HCl buffer solution to the filtered fixing solution, adjust the pH to about 7.0 (mainly to neutralize the pH drop caused by the oxidation of formaldehyde to formic acid), and obtain the body recovery solution;

[0038] (3) Add the zooplankton separated in step (1) to the body recovery solution whose pH has been adjusted in step (2), to...

Embodiment 2

[0047] Embodiment 2 Different temperature comparative experiments

[0048] (1) The pretreatment method of zooplankton samples is the same as in Example 1, except that the temperature of the water bath set in step 1(5) is different, which are 30°C, 40°C, 50°C, 70°C, and 80°C. gradient.

[0049] (2) The result is as follows image 3 As shown: the temperature is 50°C, the effect is the best; the temperature is 30°C, there is no change compared with the sample itself; the temperature is 80°C, the sample expands excessively, and cannot be used as an identification.

Embodiment 3

[0050] Embodiment 3 Different rotating speed comparative experiments

[0051] (1) The zooplankton sample pretreatment method is the same as in Example 1, the difference is that in step 3, when the shaker is used to accelerate the movement of water molecules, the comparison of the effects of accelerating the recovery of the body under different rotational speeds is set, and a control is set respectively (without a shaker) , 20r / min, 50r / min, 100r / min, 200r / min and 500r / min six groups of experiments.

[0052] (2) The result is as follows Figure 4 As shown: the control group has a certain effect, but not obvious; the 50r / min and 100r / min treatment groups have better effects, and the body stretches; the 500r / min body has a certain dehydration effect.

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Abstract

The invention discloses a body restoration and pretreatment method before the identification of a zooplankton samples. The method comprising the following steps: (1) filtering a zooplankton sample fixed in formaldehyde to obtain zooplankton and a fixed filtrate; (2) adding a pH adjuster to the fixed filtrate to adjust the pH to 6.8 to 7.2 and obtain a body restoration solution; (3) adding zooplankton to the body restoration solution to obtain a zooplankton sample A; (4) raising the temperature slowly of the zooplankton sample A obtained in the step (3) to cause the body to expand and restore,and then cooling the sample to the room temperature, and obtaining a pre-treated zooplankton sample. In order to speed up the restoration, the sample after a water bath can also be shaken in a shakerto accelerate the restoration. According to the body restoration and pretreatment method before identification of zooplankton samples, a simple and effective pretreatment method is provided for restoring the zooplankton body for subsequent observation and identification, which aims at the problem that it is difficult to observe and identify the collapsed appendages of zooplankton after being fixedby formaldehyde for a long time.

Description

technical field [0001] The invention belongs to the technical field of sample preservation, in particular to a pretreatment method before identification of zooplankton samples. Background technique [0002] Copepoda, belonging to Arthropoda, Crustacea, Copepoda subclass. It is a small crustacean with a body length of <3mm. It lives as a planktonic and parasitic animal and is distributed in ocean, freshwater or brackish water. According to statistics, there are 7,500 species of copepods in the world, of which about 4,500 species of marine copepods have been identified. Copepods are often used as environmental monitoring indicators because of their large number, wide distribution, short growth cycle, small size, and sensitivity to environmental changes. As a key link in the food chain, planktonic copepods play a connecting role in the aquatic ecosystem. As the country vigorously promotes the construction of water ecological civilization and objectively evaluates the wate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28
CPCG01N1/28
Inventor 何瑞梁志宏蒋然张心凤
Owner PEARL RIVER HYDRAULIC RES INST OF PEARL RIVER WATER RESOURCES COMMISSION
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