Supercharge Your Innovation With Domain-Expert AI Agents!

High performance liquid chromatography method for separating (R)-bendroflumethiazide and (S)-bendroflumethiazide

A technology of high performance liquid chromatography and bendroflumethiazide, which is applied in the field of chiral separation of diuretic drug bendroflumethiazide and methylchlorothiazide drug enantiomers, can solve the problem of high composition, achieve excellent separation effect and low detection cost cheap effect

Inactive Publication Date: 2019-02-19
南京盖斯夫医药科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the chiral stationary phase HPLC method requires the use of expensive chiral chromatographic columns with high composition

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High performance liquid chromatography method for separating (R)-bendroflumethiazide and (S)-bendroflumethiazide
  • High performance liquid chromatography method for separating (R)-bendroflumethiazide and (S)-bendroflumethiazide
  • High performance liquid chromatography method for separating (R)-bendroflumethiazide and (S)-bendroflumethiazide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1 separates the HPLC method of (R)-bendroflumethiazide and (S)-bendroflumethiazide

[0065] 1. Experimental instruments and materials

[0066] Shimadzu LC-20AT high performance liquid chromatograph (equipped with LC-20AT pump, SIL-20A autosampler and SPD-M20A diode array detector, Japan Shimadzu company).

[0067] Waters Xbridge C18 chromatographic column (4.6mm×250mm, 5μm) is a product of Waters Company.

[0068] (R)-bendroflumethiazide and (S)-bendoflumethiazide standard substances were purchased or made by ourselves, with a purity of not less than 95%.

[0069] The chiral reagent (1R,2R)-2-aminocyclopentanecarboxylate was purchased with a purity of not less than 95%.

[0070] Acetonitrile and tetrahydrofuran were chromatographically pure, and water was purified water produced by Hangzhou Wahaha Group Co., Ltd.

[0071] 2. Experimental methods and results

[0072] 1. Solution preparation

[0073] Preparation of mobile phase A: water-acetonitrile-tetr...

Embodiment 2

[0089] Embodiment 2 separates the HPLC method of (R)-bendroflumethiazide and (S)-bendroflumethiazide

[0090] 1. Experimental instruments and materials

[0091] Shimadzu LC-20AT high performance liquid chromatograph (equipped with LC-20AT pump, SIL-20A autosampler and SPD-M20A diode array detector, Japan Shimadzu company).

[0092] Waters Xbridge C18 chromatographic column (4.6mm×250mm, 5μm) is a product of Waters Company.

[0093] (R)-bendroflumethiazide and (S)-bendoflumethiazide standard substances were purchased or made by ourselves, with a purity of not less than 95%.

[0094] The chiral reagent (1S,2R)-2-(Boc-amino) ethyl cyclopentanecarboxylate was purchased with a purity of not less than 95%.

[0095] Acetonitrile and tetrahydrofuran were chromatographically pure, and water was purified water produced by Hangzhou Wahaha Group Co., Ltd.

[0096] 2. Experimental methods and results

[0097] 1. Solution preparation

[0098] Preparation of mobile phase A: water-acet...

Embodiment 3

[0114] Embodiment 3 separates the HPLC method of (R)-methylchlorothiazide and (S)-methylchlorothiazide

[0115] 1. Experimental instruments and materials

[0116] Shimadzu LC-20AT high performance liquid chromatograph (equipped with LC-20AT pump, SIL-20A autosampler and SPD-M20A diode array detector, Japan Shimadzu company).

[0117] Waters Xbridge C18 chromatographic column (4.6mm×250mm, 5μm) is a product of Waters Company.

[0118] (R)-Methylchlorothiazide and (S)-Methylchlorothiazide standard products were purchased or self-made, with a purity of not less than 95%.

[0119] The chiral reagent (1R,2R)-2-aminocyclopentanecarboxylate was purchased with a purity of not less than 95%.

[0120] Acetonitrile and tetrahydrofuran were chromatographically pure, and water was purified water produced by Hangzhou Wahaha Group Co., Ltd.

[0121] 2. Experimental methods and results

[0122] 1. Solution preparation

[0123] Preparation of mobile phase A: water-acetonitrile-tetrahydr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
wavelengthaaaaaaaaaa
Login to View More

Abstract

The invention discloses a high performance liquid chromatography method for separating (R)-bendroflumethiazide and (S)-bendroflumethiazide. In the method, chromatographic column: C18 chromatography column (4.6 mm x 250 mm, 5 micron); mobile phase A: water-acetonitrile-tetrahydrofuran solution with a volume ratio of 70: 25: 5, containing 20 mM (1S, 2r) -2- (Boc-amino) cyclopentanecarboxylate; mobile phase B: acetonitrile; gradient elution program: 0~5min, 0%B; 5~25min,0%~80%B; 25~30min, 80%~0%B; flow rate: 1.2mL / min; column temperature: 35DEG C. According to the high performance liquid chromatography method for separating the (R)-bendroflumethiazide and the (S)-bendroflumethiazide, through adding chiral reagent, a conventional C18 chromatographic column can be used for effectively separating enantiomers of diuretic bendroflumethiazide, separation effect is excellent, and detection cost is low.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to the chiral separation of diuretic bendrofluthiazide and methylchlorothiazide drug enantiomers. Background technique [0002] Chirality is one of the most common properties in nature. Because different enantiomers of chiral drugs often show different pharmacological activities, the research on the separation of chiral drugs has always been a hot spot in the field of pharmacy. [0003] Benzoflumethiazide and meclothiazide are diuretics that each contain a chiral center (chemical structure shown below). It has been reported in the literature that the β-cyclodextrin chiral stationary phase HPLC method was used to separate the two drug enantiomers. [0004] [0005] However, the chiral stationary phase HPLC method requires the use of expensive chiral chromatographic columns with high components. Contents of the invention [0006] The object of the present inven...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘华平李月群金荣
Owner 南京盖斯夫医药科技有限公司
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com