Construction method and detection method of hplc characteristic map of Cortex Phellodendri

A technology of characteristic map and construction method, which is applied in the construction field of HPLC characteristic map of Phellodendron phellodendron, can solve the problems that can not fully reflect the basic characteristics of traditional Chinese medicine decoction, berberine and phellodendron can not fully reflect the quality, so as to improve quality control Level, and stable internal quality, good effect of chromatographic peak shape

Active Publication Date: 2022-02-11
GUANGDONG YIFANG PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The 2015 edition of "Chinese Pharmacopoeia" uses berberine and phellodendron as the quality evaluation indicators of phellodendron, and only controlling the content of berberine and phellodendron cannot fully reflect its quality
Currently, most of the fingerprints of Phellodendron phellodendrons reported in the literature use the conventional HPLC method, and all of them only focus on the material basis of the original medicinal materials. Most of the index components are fat-soluble components as the research objects, which cannot fully reflect the material basis characteristics of traditional Chinese medicine decoctions.

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  • Construction method and detection method of hplc characteristic map of Cortex Phellodendri
  • Construction method and detection method of hplc characteristic map of Cortex Phellodendri
  • Construction method and detection method of hplc characteristic map of Cortex Phellodendri

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Embodiment 1

[0069] Embodiment 1, the construction method of the HPLC feature collection of Phellodendron Phellodendri medicinal materials

[0070] The present embodiment provides a method for building the HPLC characteristic spectrum of Cortex Phellodendri medicinal material, comprising the steps of:

[0071] (1) Preparation of reference solution

[0072] Take 0.1g of Cortex Phellodendri as reference medicinal material, put it in a conical flask with a stopper, add 50ml of 50% (v / v) methanol solution, ultrasonicate (power 250W, frequency 40kHz) for 30 minutes, let cool, shake well, filter, and take The filtrate was used as the reference substance solution of the contrast medicinal material.

[0073] Another appropriate amount of berberine hydrochloride reference substance and phellodendron hydrochloride reference substance was taken, accurately weighed, and methanol was added to make a mixed solution containing 0.1 mg per 1 ml, as the reference substance reference solution.

[0074] (...

Embodiment 2

[0087] Embodiment 2, the determination of the HPLC characteristic map construction condition of Cortex Phellodendri medicinal material

[0088] 1 Feature map

[0089] Phellodendron Phellodendri is the dry bark of Phellodendron Phellodendron, a plant of the Rutaceae Phellodendron genus, which is the base plant of traditional Chinese medicine Phellodendron Phellodendri. It mainly contains a variety of alkaloids such as berberine, magnolanine, phellodendron, palmitate and other alkaloids, as well as lactones, sterols, mucus, etc. Berberine alkaloids are the main active ingredients in Cortex Phellodendri, which have antibacterial effect, and Cortex Cortex is the characteristic component in Cortex Phellodendri. After research, HPLC was used to establish the fingerprint of Cortex Phellodendri, which provided an effective method for the quality control of Cortex Phellodendri.

[0090] 1.1 Instruments, reagents and reagents

[0091] Instruments: Waters high performance liquid chr...

Embodiment 3

[0237] Embodiment 3, the construction method of the HPLC feature collection of Phellodendron Phellodendri medicinal material

[0238] The present embodiment provides a kind of detection method of Cortex Phellodendri medicinal material, and the steps are as follows:

[0239] (1) Preparation of reference solution

[0240] Take 0.1g of Cortex Phellodendri as reference medicinal material, put it in a conical flask with a stopper, add 50ml of 50% methanol, ultrasonically treat it (power 250W, frequency 40kHz) for 30 minutes, let it cool, shake well, filter, take the filtrate as the reference medicinal material substance solution. Another appropriate amount of berberine hydrochloride reference substance and phellodendron hydrochloride reference substance were taken, accurately weighed, and methanol was added to make a mixed solution containing 0.1 mg per 1 ml, as the reference substance reference solution.

[0241] (2) Preparation of the test solution

[0242] Take 0.1g of the ...

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Abstract

The invention relates to a construction method and a detection method of an HPLC characteristic map of Cortex Phellodendri. The construction method includes: preparation of reference substance solution: preparing Phellodendron phellodendron reference substance reference solution, berberine hydrochloride and phellodendron hydrochloride reference substance reference substance solution; preparation of test product solution: preparing phellodendron phellodendron medicinal material for test solution, decoction for testing Product solution; high-performance liquid chromatography test: draw described contrast medicinal material reference substance solution, contrasting substance reference substance solution, medicinal material need testing solution, decoction need testing solution, inject liquid chromatograph, compare gained medicinal material need testing collection of illustrations With the spectrum of the decoction test sample, determine the common peak of water solubility, and obtain the HPLC characteristic spectrum of Phellodendron Phellodendri. The HPLC characteristic spectrum obtained by this construction method can not only qualitatively and quantitatively analyze the quality of Cortex Phellodendri, but also use it as a reference to reflect the quality of Phellodendron Phellodendri standard decoction.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a construction method and a detection method of an HPLC characteristic map of Cortex Phellodendri. Background technique [0002] Cortex Phellodendri Phellodendron chinense Schneid. of dry bark. It has the effects of clearing away heat and dampness, purging fire and removing steam, detoxifying and treating sores, and is used for damp-heat diarrhea, jaundice, red urine, vaginal itching, hot stranguria, astringent pain, bone steaming, labor heat, night sweats, sore swelling, eczema Diseases such as eczema. The main active ingredients of Cortex Phellodendri are alkaloids, including berberine, phellodendron, jatrorrhizine, and palmatine. Among them, berberine and phellodendron have pharmacological effects such as anti-inflammatory, antibacterial, antipyretic and analgesic, and are the main ones. Pharmacologically active ingredients. It has a strong clinical applicatio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/36
CPCG01N30/02G01N30/06G01N30/36
Inventor 霍文杰王闽予魏梅程学仁黄小丹潘礼业陈万发陈向东
Owner GUANGDONG YIFANG PHARMA
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