Detection reagent for detecting triiodothyronine
A technology of triiodine thyroid gland and detection reagent, which is applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of poor stability, low detection cost, poor repeatability, etc., and achieves good repeatability, low production cost, Accurate effect
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Embodiment 1
[0015] The preparation method of embodiment 1 reagent one:
[0016] Step 1: Take 0.5 mL of well-mixed magnetic particles (concentration: 1%) and place them in a microtube, place them on a magnetic base for 1 minute, and remove the supernatant. Take 1mL of the reaction solution and add it into the above microtube, mix well and stir for 30 minutes. Place on magnetic stand for 1 min and remove supernatant. Take 1 mL of binding buffer and add it to the above microtube, mix thoroughly, take 100 μg of triiodothyronine antigen, and place the microtube in a rotary mixer to stir for 3 hours. Add 1mL of blocking solution, mix well and stir for 30 minutes, place on the magnetic base for 1 minute, and remove the supernatant. Add 1 mL of wash buffer, mix well and remove the supernatant. Take 1 mL of tris buffer solution containing protein stabilizer and add it into the microtube and mix thoroughly to obtain the magnetic particle suspension coated with triiodothyronine antigen. Add the ...
Embodiment 2
[0022] The preparation of embodiment 2 reagent two:
[0023] Step 1: (1) Take 1 mg of triiodothyronine antibody, dialyze with binding buffer for 24 hours, and prepare the concentration of 1 mg / mL with binding buffer. (2) Dissolve 1 mg of acridinium ester in 1 mL of dimethyl sulfoxide to obtain a concentration of 1 mg / mL. (3) Add 80 μL of the above-mentioned acridinium ester solution into the antibody solution, and stir thoroughly for 2 hours. (4) Dialyze the solution in step (3) with storage buffer for 24 hours, then add 50% glycerol and mix thoroughly. Add the specified amount of phosphate buffer and mix well to obtain reagent 2.
[0024] Step 2: Determination of the concentration of reagent 2:
[0025] Dilute the acridinium ester labeled with triiodothyronine antibody at 1:250, 1:500, 1:1000 and 1:2000 respectively, and pass the detection by diluting the high-value samples to concentrations of 8 ng / mL and 4 ng / mL respectively. mL, 2ng / mL, 1ng / mL, 0.5ng / mL samples and mat...
Embodiment 3
[0029] The preparation of embodiment 3 calibrator
[0030] Take the triiodothyronine antigen and add it to the phosphate buffer saline buffer containing protein stabilizer and mix well, then use the national standard to assign the value.
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