Tumor blood marker and application thereof

A tumor and blood sample technology, applied in the field of tumor blood markers, can solve problems such as unseen correlation

Pending Publication Date: 2019-03-01
SHANDONG ZEJI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] After investigation, there is no report on the correlation between the content of GAPDH in the serum / plasma of tumor patients and the tumor, and its relationship with the occurrence and development of tumor needs further study

Method used

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  • Tumor blood marker and application thereof
  • Tumor blood marker and application thereof
  • Tumor blood marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] The plasma of healthy people and patients with different types of tumors was collected, and the concentration of GAPDH in the plasma was detected by Western blot, which proved that GAPDH can be used as a tumor marker.

[0078] The specific experimental method is as follows:

[0079] 1. Sample Collection

[0080] Blood was collected from healthy people and patients with pathologically diagnosed gastric cancer, lung cancer, and liver cancer, and the collected blood was placed in an anticoagulant tube, which was inverted 8-10 times. Plasma was separated by centrifugation (800-1000 rpm, 10 minutes), and the plasma was divided into EP tubes (50 μL each), and quickly stored at -20°C for cryopreservation.

[0081] 2. Sample Processing

[0082] After each plasma sample was diluted 20 times, 5× loading buffer was added and heated at 100°C for 10 minutes.

[0083] 3. Sample detection

[0084] GAPDH in the treated samples was detected by Western blot method.

[0085] 4. Resul...

Embodiment 2

[0088] The blood of healthy people and patients with liver cancer was collected, and the concentration of GAPDH in serum was detected by enzyme-linked immunosorbent assay (Elisa), and the effectiveness of GAPDH in the diagnosis of liver cancer was evaluated.

[0089] The specific experimental method is as follows:

[0090] 1. Sample selection

[0091]Healthy people: people who have not been diagnosed by biochemical, imaging or pathological methods and are temporarily considered to be non-tumor patients.

[0092] Liver cancer patients: patients diagnosed with liver cancer by pathological diagnosis, including different types and stages of liver cancer.

[0093] 2. Sample Collection

[0094] Collect the blood of healthy people and liver cancer patients respectively, collect the blood and let it stand at room temperature for 20 minutes, centrifuge the serum (800-1000 rpm, 10 minutes), divide the serum into EP tubes (50 μL each), and place them quickly in Freeze at -20°C.

[00...

Embodiment 3

[0108] On the one hand, the serum of healthy people and lung cancer patients was collected, the concentration of GAPDH in the serum was detected by Elisa method, and the effectiveness of GAPDH in the diagnosis of lung cancer was evaluated. On the other hand, follow up some lung cancer patients, collect blood samples before and after treatment, detect the changes of GAPDH concentration in serum, and evaluate the effectiveness of GAPDH in disease monitoring and curative effect evaluation.

[0109] The specific experimental method is as follows:

[0110] 1. Sample selection

[0111] Healthy people: people who have not been diagnosed by biochemical, imaging or pathological methods and are temporarily considered to be non-tumor patients.

[0112] Lung cancer patients: patients diagnosed with lung cancer by pathological diagnosis, including different types and stages of lung cancer.

[0113] 2. Sample Collection

[0114] Collect blood from healthy people and lung cancer patients,...

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Abstract

The invention provides a tumor blood marker and an application thereof and particularly provides an application of a reagent for detecting GAPDH (glyceraldehyde 3-phosphate dehydrogenase) in a blood sample to preparation of composition for tumor screening, tumor risk assessment of a subject, tumor progression stage division, tumor curative effect identification and / or detection of tumor progression risk analysis. The invention further provides a kit and a detection method for GAPDH concentration of the blood sample.

Description

technical field [0001] The present invention relates to a tumor blood marker and its application, in particular to the diagnosis and prognosis of liver cancer, lung cancer, breast cancer, gastric cancer, esophageal cancer, colorectal cancer and other tumors by detecting the content of GAPDH in human blood Techniques for evaluation and efficacy monitoring. The marker GAPDH of the present invention can be used including but not limited to tumor screening, risk assessment of subjects developing tumors, differentiation of tumor progression stages, identification of tumor therapeutic efficacy, and risk analysis of tumor progression. Background technique [0002] Malignant tumors are common and frequently-occurring diseases worldwide, and one of the major diseases that endanger human health. In 2015, there were about 4.292 million new cancer cases in China, an average of 12,000 new cases per day; at the same time, about 2.814 million people died of cancer, with an average of 7,50...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/574
CPCG01N33/57484G01N33/577G01N2800/50G01N33/574G01N2800/52G01N2800/54G01N2800/56C12Y102/01012G01N2333/90203G01N33/57488G01N33/57407G01N33/6803
Inventor 王泽宋
Owner SHANDONG ZEJI BIOTECH CO LTD
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