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System for predicting lignified pectoral muscle of broiler chicken through blood marker and application

A blood marker and lignification technology, which is applied in the field of biochemistry, can solve the problems of low accuracy, large influence of subjective factors, and difficulty in determining the diagnostic accuracy of lignified pectoral muscle, etc., and achieve the effect of rapid detection and high judgment accuracy

Pending Publication Date: 2022-03-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the actual production of enterprises, the judgment and grading of lignified chicken breast meat are mainly based on the experience of workers, but due to the great influence of subjective factors in the evaluation criteria, it is difficult to use it as a fast and accurate judgment method; using near-infrared spectroscopy or optical coherence Tomography imaging technology distinguishes normal meat from lignified chicken breast meat, which can only be judged after slaughtering and segmentation, and cannot realize the evaluation or diagnosis of lignified breast muscle chickens in vivo; while ultrasonic imaging technology is used for early prediction of lignified breast muscle, but echo The correlation coefficient between reflexivity and lignified breast muscle score is low, and it is difficult to determine the accuracy of lignified breast muscle diagnosis; in addition, the patent publication No. CN113207803A discloses the use of blood creatine kinase levels to predict lignified breast lesions in broilers, but its accuracy The accuracy of the diagnosis was 68%, and the accuracy of diagnosis was not high. In addition, many factors such as feeding management, disease, and stress in broiler production would cause changes in the blood creatine kinase level. Guiding the diagnosis of live lignified breast muscle chickens in production

Method used

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  • System for predicting lignified pectoral muscle of broiler chicken through blood marker and application
  • System for predicting lignified pectoral muscle of broiler chicken through blood marker and application
  • System for predicting lignified pectoral muscle of broiler chicken through blood marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Blood biochemical indicators are used to judge or predict the performance of lignified breast muscle white feather broilers. The specific steps are as follows:

[0028] 1. Test sample preparation

[0029] A total of 164 healthy 41-day-old male Albayga broilers were randomly selected. The feeding management and nutritional level were the same. Blood was collected from the wing veins with vacuum blood collection tubes without anticoagulants. Blood gas analysis was performed immediately after blood samples were collected.

[0030] Broiler chickens were stunned by electric shock, bled and slaughtered, the complete pectoralis major muscle on the right side was peeled off, and cut into 0.2 cm × 0.2 cm × 0.5 cm specific traits along the muscle fiber direction, rinsed with pre-cooled normal saline, soaked in more than 4% Fix in polyoxymethylene solution for tissue sectioning and observation of muscle fiber structure.

[0031] 2. Test method

[0032] 2.1 Blood gas analysis

...

Embodiment 2

[0065] To verify the accuracy of judgment, the specific steps are as follows:

[0066] 1. Test sample

[0067] Random sampling, the sampling object is 38-43 days old Arbor Aegis broiler chickens, the total number of samples in the verification set is 117, and the sample collection and index determination are completed within one month.

[0068] 2. Test method

[0069] 2.1 Vacuum blood collection tubes without anticoagulants were used to collect blood from the wing vein, and blood gas analysis was performed immediately after the blood samples were collected. The right pectoralis major muscle was collected for pathological section staining and observation, which was used to determine the lignification of broiler breast muscle. The specific analysis and measurement methods were the same as in Example 1.

[0070] 2.2 In the verification set, the accuracy of judgment of target blood markers was calculated according to the prediction probability given by SPSS22.0 software.

[007...

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Abstract

The invention discloses a system for predicting lignified pectoral muscle of broiler chicken through blood markers and application, a regression equation for predicting the lignified pectoral muscle of the broiler chicken is established through the blood markers, whether the pectoral muscle of the broiler chicken is lignified or not is predicted according to the regression equation, and the blood markers comprise any one or three of the content of pO2, the content of sO2 and the content of pCO2 in blood. The system provided by the invention comprises a data acquisition module used for acquiring the content of blood markers in broiler chicken sample blood; the data processing module is used for substituting the received blood marker content data in the data acquisition module into a regression equation for data processing to obtain a regression value; the data studying and judging module is used for receiving the regression value obtained by processing of the data processing module and studying and judging whether the breast muscle of the meat chicken is lignified or not; and the output module is used for outputting the research and judgment result and displaying the research and judgment result on the processor.

Description

technical field [0001] The invention relates to a system and application of blood markers for predicting lignified breast muscles of broiler chickens, in particular to a method for individually or jointly judging or predicting chickens with lignified breast muscles by three blood markers, belonging to the technical field of biochemistry. Background technique [0002] Genetic selection and intensified production of modern broiler breeds have greatly increased broiler growth rate and breast muscle yield, but this has also led to increased stress sensitivity and some spontaneous or idiopathic muscle lesions. Occurs, resulting in the decline of chicken quality and the production of heterogeneous chicken. Among them, woody meat is a new type of heterogeneous chicken breast meat with a high incidence rate and a wide range of influences newly discovered in recent years. The lignified breast muscle of broiler chickens is a progressive lesion, which can be seen at the earliest age o...

Claims

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Application Information

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IPC IPC(8): G01N33/49G01N21/84A01K67/02G06F17/18
CPCG01N33/4925G01N21/84G06F17/18A01K67/02
Inventor 邢通赵雪高峰
Owner NANJING AGRICULTURAL UNIVERSITY
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