System for predicting lignified pectoral muscle of broiler chicken through blood marker and application
A blood marker and lignification technology, which is applied in the field of biochemistry, can solve the problems of low accuracy, large influence of subjective factors, and difficulty in determining the diagnostic accuracy of lignified pectoral muscle, etc., and achieve the effect of rapid detection and high judgment accuracy
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Embodiment 1
[0027] Blood biochemical indicators are used to judge or predict the performance of lignified breast muscle white feather broilers. The specific steps are as follows:
[0028] 1. Test sample preparation
[0029] A total of 164 healthy 41-day-old male Albayga broilers were randomly selected. The feeding management and nutritional level were the same. Blood was collected from the wing veins with vacuum blood collection tubes without anticoagulants. Blood gas analysis was performed immediately after blood samples were collected.
[0030] Broiler chickens were stunned by electric shock, bled and slaughtered, the complete pectoralis major muscle on the right side was peeled off, and cut into 0.2 cm × 0.2 cm × 0.5 cm specific traits along the muscle fiber direction, rinsed with pre-cooled normal saline, soaked in more than 4% Fix in polyoxymethylene solution for tissue sectioning and observation of muscle fiber structure.
[0031] 2. Test method
[0032] 2.1 Blood gas analysis
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Embodiment 2
[0065] To verify the accuracy of judgment, the specific steps are as follows:
[0066] 1. Test sample
[0067] Random sampling, the sampling object is 38-43 days old Arbor Aegis broiler chickens, the total number of samples in the verification set is 117, and the sample collection and index determination are completed within one month.
[0068] 2. Test method
[0069] 2.1 Vacuum blood collection tubes without anticoagulants were used to collect blood from the wing vein, and blood gas analysis was performed immediately after the blood samples were collected. The right pectoralis major muscle was collected for pathological section staining and observation, which was used to determine the lignification of broiler breast muscle. The specific analysis and measurement methods were the same as in Example 1.
[0070] 2.2 In the verification set, the accuracy of judgment of target blood markers was calculated according to the prediction probability given by SPSS22.0 software.
[007...
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