Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Spatial light modulator based hyperspectral confocal microscopes and methods of use

A confocal, phase-modulated technology used in imaging to solve problems such as expensive, impossible, and cumbersome

Active Publication Date: 2019-03-01
VERILY LIFE SCI LLC
View PDF5 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Changing the degree of optical sectioning may require physically removing and replacing the disc, which can be cumbersome, expensive, or even impossible in most systems

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Spatial light modulator based hyperspectral confocal microscopes and methods of use
  • Spatial light modulator based hyperspectral confocal microscopes and methods of use
  • Spatial light modulator based hyperspectral confocal microscopes and methods of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] The disclosed embodiments relate to systems and methods for achieving confocal imaging that allow for fast image acquisition and efficient use of excitation light. Embodiments of the present disclosure may be implemented using a microscope, such as a fluorescence, confocal, transmission or reflection microscope, with one or more 2-D imaging devices (eg, CCD or CMOS sensors or cameras). Alternatively, suitable optical components may be used to construct optical imaging systems according to embodiments of the present disclosure.

[0021] Rather than using a Nipkow disk with predetermined pinholes and / or microlens arrays, embodiments of the present disclosure allow the use of programmable artificial pinholes with adjustable size and spacing to acquire 2-D images of the focal plane in the sample . Multiple 2-D images can be acquired at multiple focal planes and computationally reconstructed to obtain a 3-D or virtual volumetric image of the sample. Additionally, embodimen...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Systems and methods for confocal imaging are described. In one implementation, a confocal imaging system (100) includes a light source (110) configured to emit excitation light (10) having one or morewavelengths, a sample holder (180) configured to hold a sample, a two-dimensional (2-D) imaging device (190), and an optical system comprising: a first spatial light modulator (140) and a second spatial light modulator (150), wherein the optical system is configured to (i) collimate the excitation light (10); (ii) apply, using the first spatial light modulator (140), a predetermined phase modulation pattern to the collimated excitation light (12); (iii) use the phase-modulated collimated excitation light (14) as an excitation pattern to illuminate the sample, wherein the excitation pattern islocated at a Fourier plane relative to a focal plane within the sample; (iv) collect emission light (18) from the focal plane within the sample; and (v) direct the collected emission light (20) to the imaging device (190) via the second spatial light modulator (150) such that the focal plane within the sample is conjugate to a pinhole pattern formed by pixels of the second spatial light modulator(150).

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of U.S. Provisional Patent Application No. 62 / 342,256, filed May 27, 2016, and U.S. Nonprovisional Patent Application No. 15 / 607,455, filed May 27, 2017, the contents of which are incorporated herein by reference Application. technical field [0003] The present disclosure relates generally to the field of imaging and microscopy systems and methods. More specifically, but not limited to, the disclosed embodiments relate to systems and methods for confocal imaging through the use of spatial light modulators. Background technique [0004] Fluorescence microscopy uses the principle of fluorescence rather than light absorption, phase or interference effects to highlight inspected structures. For example, it can be applied in biology and other disciplines to characterize samples. In fluorescence microscopy, fluorophores or other optical labels in a sample are excited by an excitation b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N21/64G02B21/00G02B21/16G01J3/02
CPCG01J3/10G01J3/433G02B21/004G02B21/0076G01N21/6458G02B21/16G01N2021/6419G01N2021/6471G01N2201/0675G01J3/0229G01J3/2823G01J3/4406G02B21/0032G02B21/0036
Inventor C-H.吴C.桑托里S.辛哈
Owner VERILY LIFE SCI LLC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com