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Biomarker for severe asthma and application thereof

A technology for severe asthma, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, etc., can solve the problems of severe asthma epidemiology, genetic research pathological characteristics and pathogenesis are not completely clear, and achieve early realization Effects of diagnosis and mortality reduction

Inactive Publication Date: 2019-03-08
CHANGZHOU NO 2 PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the epidemiology, genetic research, pathological characteristics and pathogenesis of severe asthma have not been fully clarified, and further research is needed

Method used

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  • Biomarker for severe asthma and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1 Screening Gene Markers Related to Asthma

[0061] 1. Sample collection

[0062] 3 ml of peripheral venous blood was collected from 3 normal persons, 3 normal adult asthmatics, and 3 severe adult asthmatics. All patients gave informed consent, and all the above specimens were obtained with the consent of the ethics committee.

[0063]Patient Inclusion Criteria:

[0064] Ordinary asthma patients meet the diagnostic criteria for bronchial asthma in the 2016 guidelines for the prevention and treatment of bronchial asthma; severe asthma meets the "Chinese Expert Consensus on the Diagnosis and Treatment of Severe Asthma"; the three groups of samples are all taking any drugs in a short period of time; (4) Age > 18 years old .

[0065] Patient Exclusion Criteria:

[0066] Patients with co-infection, pulmonary embolism, chronic bronchitis, tuberculosis and blood system diseases; patients with abnormal liver function.

[0067] 2. RNA sample preparation and quality a...

Embodiment 2

[0078] Example 2 QPCR sequencing to verify the differential expression of the ENSG00000241587 gene

[0079] 1. According to the detection results of high-throughput sequencing, the ENSG00000241587 gene was selected for large-sample QPCR verification. According to the method in Example 1, blood samples were collected from 45 normal asthma patients, 38 severe asthma patients, and 40 normal subjects.

[0080] 2. RNA extraction

[0081] Total RNA was extracted using the RNA extraction kit from Promega, and the specific steps are detailed in the instruction manual.

[0082] 3. Reverse transcription

[0083] The FastQμant cDNA First Strand Synthesis Kit of TIANGEN was used for reverse transcription. For details, please refer to the instruction manual.

[0084] 4. QPCR detection

[0085] 4.1 Primer design

[0086] QPCR amplification primers were designed according to the sequences of the gene encoding ENSG00000241587 and the GAPDH gene, and were synthesized by Shanghai Sangon Bi...

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Abstract

The invention discloses a biomarker for severe asthma and application thereof. The biomarker is ENSG00000241587. The expression level of lncRNA (long non-coding Ribonucleic Acid) in blood samples of normal people, common asthma patients and severe asthma patients is detected, finding that the ENSG00000241587 has remarkable difference in the severe asthma for the first time, which shows that the ENSG00000241587 can be used as a detection target to be applied to auxiliary diagnosis of the severe asthma.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a severe asthma biomarker and application thereof, specifically the biomarker is ENSG00000241587. Background technique [0002] Bronchial asthma (referred to as asthma) is a chronic airway inflammatory disease involving eosinophils, mast cells, neutrophils, and airway epithelial cells. Recurrent wheezing, shortness of breath, chest tightness, coughing and other symptoms, the vast majority of asthma patients can effectively relieve asthma symptoms after being given anti-inflammatory drugs and bronchodilators, about 5% to 10% of asthma patients even if given multiple drug combination therapy Symptoms are still difficult to control, which is called severe asthma (Severe asthma), but it consumes a lot of medical and health resources, which makes the cost of asthma treatment increase year by year. [0003] Although severe bronchial asthma accounts for a small proportion of asthmatic patients...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
Inventor 张倩孙赟殷小伟
Owner CHANGZHOU NO 2 PEOPLES HOSPITAL
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