Pesticide residue and nitrite detection device and method based on scanning code paper chip

Abstract

The invention relates to a pesticide residue and nitrite detection device and method based on a scanning code paper chip that uses microfluidic technology and image processing technology to identify, detect and record pesticide residues and nitrite harmful substances. The detection chamber of the chip can be heated A multi-layer microfluidic paper chip is placed inside. The multi-layer microfluidic paper chip is composed of five layers glued together from top to bottom. Each layer has a chip barcode detection area, and the dropper drips the The mixed liquid is added to the chip inlet, and the barcode detection area of ​​each layer shows different colors according to the composition of the sample mixed liquid. The smart mobile device images and scans the color results of the multi-layer microfluidic paper chip for detection. As a result, the principle of inhibition of acetylcholinesterase by organophosphorus pesticides and the diazonium coupling reaction of aminobenzenesulfonic acid and N-1-naphthylethylenediamine under acidic conditions were used to integrate automatic sampling, intelligent heating, reaction, and display. Color and result analysis record processing in one.

Description

technical field [0001] The invention relates to a portable device for identifying, detecting and recording pesticide residues and nitrite harmful substances by adopting microfluidic technology and image processing technology. Background technique [0002] At present, chemical fertilizers and pesticides are still the main means to prevent crop diseases and increase crop and soil nutrients. However, pesticide residues are extremely harmful to the human body, and residual toxins are not suitable for detection. Pesticide poisoning can cause cancer and death. It is widely used in water bodies and The chemical substances left in the soil have also caused a series of environmental pollution problems. Nitrogen-containing fertilizers have a significant effect on improving soil nutrients, and excessive or excessive use of nitrogen fertilizers will not only easily cause water pollution, but also easily cause a significant increase in the content of nitrate and nitrite in agricultural p...

AI Technical Summary

Problems solved by technology

[0005] Aiming at the defects of the traditional detection card used in the detection of pesticide residues and nitrite, the present invention proposes an intelligent, automatic, high-throughput and portable detection of pesticide residues and nitrite based on a multi-layer paper-based microfluidic chip. The salt detection device and the detection method of the device realize multiple detections of a single index in one sample injection, so as to solve the problems of low automation, cumbersome detection procedures, large chance of detection results and low accuracy of the existing rapid detection cards , and more importantly, optimize the process of processing, analyzing and recording the experimental results, get rid of the process of strongly relying on manual analysis, processing and recording, and realize food safety anytime, anywhere, fast, convenient, automatic, intelligent and high accuracy detection records and analyze

Images