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A kind of in vitro culture method of young embryo of medicinal plant Veratrum

A technology of in vitro cultivation of medicinal plants, applied in the field of biological seedlings, can solve the problems of difficult survival of immature embryos, and achieve the effects of high survival rate, simple operation, and enhanced activity

Inactive Publication Date: 2021-06-08
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The invention solves the problem that immature immature embryos of Veratrum are difficult to survive due to the secretion of polyphenols and other toxic compounds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A pharmaceutical plant gamphubstormation process, including the following steps:

[0023] (1) The pointer is the lavess period, the flowering period, the result period in July, the scorpion is growing in the northern big pot of 200 meters from the northern balance of Jilin City, Xing Anza is growing in the red stone The original forest under the Wangjia shop village of Zizi Town, collected unsuccessful sauce capsules on July 30, dialing immature seeds from the pods, and sterilized;

[0024] (2) Dialing the seed embryo in the seed under anatomical mirror, then put in the soaking solution, soaking treatment for 30 min, wherein the soaking liquid is made of a component of the following weight: 2 parts 1, 2-ring Diamine tetracetic acid, 1 part 24 - olerapene lactone, 7 parts of beet base, 130 water;

[0025] (3) The seed embryos treated after step (2) were added to the seed embryonic culture medium, and 24 ° C was dark culture, and the seed embryo medium was produced by MS medi...

Embodiment 2

[0034] A pharmaceutical plant gamphubstormation process, including the following steps:

[0035] (1) The pointer is the lavess period, the flowering period, the result period in July, the scorpion is growing in the northern big pot of 200 meters from the northern balance of Jilin City, Xing Anza is growing in the red stone The original forest under the Wangjia shop village of Zizi Town, collected unsuccessful sauce capsules on July 30, dialing immature seeds from the pods, and sterilized;

[0036] (2) Dip the seed embryo in the seed by anatomical knife under anatomical mirror, then put in the soaked solution, soaking treatment for 40 min, wherein the soaking liquid is made of a component of the following: 5 parts 1, 2-ring Diamine tetracetic acid, 3 parts of 24-oleraneuclease, 10 parts of toklakoid, 140 parts of hydrochloride;

[0037] (3) The seed embryos treated after step (2) were added to the seed embryonic culture medium, and the culture was dark at 27 ° C, of ​​which the see...

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Abstract

The invention discloses a method for in vitro cultivation of immature embryos of the medicinal plant Veratrum, which comprises the following steps: (1) collecting immature capsules of Veratrum, removing immature seeds from the fruit pods, and performing sterilization; (2) Pull out the immature embryos in the seeds with a scalpel under a dissecting microscope, then put them into the soaking liquid, and soak for 30-40min; (3) add the immature embryos processed through step (2) into the immature embryo culture medium, Culture in dark at 24-27°C; (4) When the young embryo grows into a 4-5cm seedling, move the seedling to the rooting medium, and when the root system of the seedling is well developed, transplant it into peat soil, wherein the rooting medium is cultured by MS base, 60-70mg / L iron salt, 0.8-1.2mg / L indolebutyric acid, 2-5mg / L sodium hyaluronate. The invention provides a method for in vitro cultivation of the immature embryos of the medicinal plant Veratrum, which is simple to operate, can cultivate immature embryos into seedlings, and has a high survival rate, wherein the average survival rate of the immature embryos can reach about 93%. The average rooting rate of seedlings can reach about 95%.

Description

Technical field [0001] The invention belongs to the field of biomymount, and more particularly to a pharmaceutical phytotype epithelial epithelial culture method. Background technique [0002] Pharmaceutical plant grouse active alkaloids, mainly used in traditional Chinese medicine in my country to treat diseases such as stroke, dysentery, jaundice, headache, chronic malaria, etc. At present, more than 100 steroidal alkaloids have been separated from gush. The latest study shows that the pneumatic biological alkaloid rhodium and its derivatives can effectively block the cancer Sonic Hedgehog signaling channel, which has a significant therapeutic effect on myelosalabaroma, basal cell carcinoma, small cell lung cancer, is huge Anti-cancer drugs for market potential. Due to the complexity of the chemical structure of the cyclic, although the study of cypperfaming and cyclic murder semi-synthesis has made certain progress, the artificial ultimate synthesis of cyclizing is still relat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 马瑞于志晶尚丽霞蔡勤安王丹任鹏飞刘晓蕊
Owner JILIN ACAD OF AGRI SCI
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