A kind of food biomimetic nano drug delivery system based on egg white and its preparation method and application

A biomimetic nanotechnology, egg white technology, applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, etc. In order to improve the bioavailability, good application prospects and excellent anti-tumor effect of the drug system

Active Publication Date: 2021-06-29
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the use of egg white to deliver drugs, and the application of egg white to deliver drugs in tumor treatment still faces many challenges. Then solidify by heating or deformation, while the adsorption of emulsifier on the interface is dynamic adsorption, which is prone to desorption, which is manifested in phase separation, droplet flocculation, Oswald ripening, etc. during processing and storage. Instability, the instability of the drug-loaded system prepared by the above method limits its application in the pharmaceutical industry
Moreover, its particle size is usually large, it cannot effectively enter the tumor tissue, and its targeting in vivo needs to be further improved, etc.
Furthermore, in recent years, the safety issues of small molecule surfactants have also aroused consumers' concerns

Method used

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  • A kind of food biomimetic nano drug delivery system based on egg white and its preparation method and application
  • A kind of food biomimetic nano drug delivery system based on egg white and its preparation method and application
  • A kind of food biomimetic nano drug delivery system based on egg white and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0066] Example 1 Preparation of a food biomimetic nano drug delivery system (PTX / EW-NPs) based on egg white

[0067] 1, preparation method, comprises the following steps:

[0068] (1) Preparation of egg white aqueous solution: separate egg white from eggs with an egg white separator, remove and discard the precipitate after centrifugation, freeze-dry the egg white solution to obtain egg white powder, dissolve it in PBS buffer solution, and obtain egg white aqueous solution ;

[0069] (2) Dissolve the stabilizer DSPEG-PEG3000 and appropriate amount of paclitaxel (PTX) powder in ethanol to obtain the corresponding ethanol solution;

[0070] (3) Preparation of PTX / EW-NPs: Add the ethanol solution in the above step (2) dropwise to 0.5% (W / V) egg white aqueous solution under the condition of 0°C ice bath, and perform ultrasonic dispersion treatment at the same time, and the ultrasonic power is controlled at 20 kHz, the amplitude is controlled at 25%, the amount of stabilizer adde...

Embodiment 2

[0071] Example 2 Preparation of a food biomimetic nano drug delivery system based on egg white

[0072] 1, preparation method, comprises the following steps:

[0073] (1) Preparation of egg white aqueous solution: separate egg white from eggs with an egg white separator, remove and discard the precipitate after centrifugation, freeze-dry the egg white solution to obtain egg white powder, dissolve it in PBS buffer solution, and obtain egg white aqueous solution ;

[0074] (2) Dissolve the stabilizer DSPEG-PEG 500 and appropriate amount of methotrexate powder in ethanol to obtain the corresponding ethanol solution;

[0075] (3) Preparation of PTX / EW-NPs: Under the condition of ice bath at 2°C, add the ethanol solution in the above step (2) dropwise to 1% (W / V) egg white aqueous solution, and perform ultrasonic dispersion treatment at the same time, and the ultrasonic power is controlled at 25 kHz, the amplitude is controlled at 30%, the amount of stabilizer added is 6% of the ...

Embodiment 3

[0076] Example 3 Preparation of a food biomimetic nano drug delivery system based on egg white

[0077] 1, preparation method, comprises the following steps:

[0078] (1) Preparation of egg white aqueous solution: separate egg white from eggs with an egg white separator, remove and discard the precipitate after centrifugation, freeze-dry the egg white solution to obtain egg white powder, dissolve it in PBS buffer solution, and obtain egg white aqueous solution ;

[0079] (2) Dissolve the stabilizer DSPEG-PEG 5000 and appropriate amount of camptothecin powder in ethanol to obtain the corresponding ethanol solution;

[0080] (3) Preparation of PTX / EW-NPs: Add the ethanol solution of the above step (2) dropwise to 0.2% (W / V) egg white aqueous solution under the condition of 4°C ice bath, and carry out ultrasonic dispersion treatment at the same time, and the ultrasonic power is controlled at 15 kHz, the amplitude is controlled at 20%, the amount of stabilizer added is 30% of th...

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Abstract

The invention discloses an egg white-based food biomimetic nano drug delivery system as well as a preparation method and application thereof. The preparation method comprises the following steps: S1. Dissolving the stabilizer and the drug in a solvent to obtain a mixed solution; S2. Under the condition of an ice bath, uniformly disperse the above mixed solution into the egg white solution at a low temperature to obtain a uniform particle size, stable Nano solution, ultrafiltration, that is. The present invention uses egg white as the main material, and by mixing stabilizers, therapeutic drugs and egg white solution through low-temperature dispersion, the particle size of the whole system is suitable, and the stability in vitro is good. The acidic environment of the tumor can trigger the release of the system, which has great It has good biological safety, biodegradability, circulation stability and tumor targeting, and has the characteristics of effectively inhibiting the growth of tumor cells, and can realize the synergistic anti-tumor effect of oral and intravenous administration routes, and has great potential in the field of biomedicine Good application prospects.

Description

technical field [0001] The invention belongs to the technical fields of functional polymer materials and biomedicine. More specifically, it relates to an egg white-based food biomimetic nano drug delivery system and its preparation method and application. Background technique [0002] Cancer is one of the major diseases that endanger human health and cause human death. Clinically, advanced cancer patients are mainly treated with chemotherapy. In recent years, the research on chemotherapeutic drugs has become more and more mature, and they have also shown good effects in clinical anti-tumor therapy. However, the side effects and administration methods of chemotherapy drugs have always been the important reasons restricting their further development. Oral administration is undoubtedly more favored by patients as a drug route that is easily accepted by patients. However, since a large proportion of chemotherapy drugs are hydrophobic drugs, their oral absorption is poor, and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/46A61K31/337A61K31/4745A61K31/519A61K31/704A61P35/00
CPCA61K9/0002A61K9/5176A61K31/337A61K31/4745A61K31/519A61K31/704A61P35/00
Inventor 吴钧黄骏顾志鹏
Owner SUN YAT SEN UNIV
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