Method for continuous production of 1,3-propanediol by microorganisms

A technology of propylene glycol and microorganisms, which is applied in the field of bioengineering, can solve the problems of low concentration of 1,3-propanediol products and cannot significantly improve production efficiency, and achieve the effect of realizing repeated utilization and improving production efficiency

Active Publication Date: 2019-03-19
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above method has the advantages of simplifying the process and shortening the culture period, due to the low concentration of the 1,3-propanediol product in the fermentation system, the technical solution for the batch fermentation mode cannot significantly improve the production efficiency

Method used

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  • Method for continuous production of 1,3-propanediol by microorganisms

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Strain cultivation: take 70mL of activated bacterial liquid, mix it with 630mL of seed medium in a 1L fermenter, and carry out micro-aerobic culture (without nitrogen gas); the culture conditions are: culture temperature is 37°C, stirring speed is 200rpm , the pH was controlled at 7.0, and the fermented seed liquid was obtained after culturing for 18 hours.

[0033] (2) Continuous fermentation: The volume of the fermenter is 15L, and the liquid volume is 7L.

[0034] a. Take 700mL of fermented seed liquid and insert it into 6.3L of fermentation medium (medium-density chitosan with a molecular weight of 150,000 is added to the fermentation medium, and the addition amount is 1g / L).

[0035] b. Carry out anaerobic fermentation, and add glycerol during the fermentation process to maintain the glycerol concentration in the fermentation system at 40g / L; control the culture temperature during the fermentation process to be 37°C, pH to be 7.0, stirring speed to be 400rpm, a...

Embodiment 2

[0039] (1) Strain cultivation: Take 35mL of activated bacterial liquid, mix it with 665mL of seed medium in a 1L fermenter, and carry out micro-aerobic culture (without nitrogen inflow); the culture conditions are: culture temperature is 35°C, stirring speed is 100rpm , the pH was controlled at 7.0, and the fermented seed liquid was obtained after culturing for 24 hours.

[0040] (2) Continuous fermentation: The volume of the fermenter is 15L, and the liquid volume is 7L.

[0041] a. Take 350mL of fermented seed liquid and insert it into 6.65L of fermentation medium (medium-density chitosan with a molecular weight of 150,000 is added to the fermentation medium, and the amount added is 3.5g / L).

[0042] b. Carry out anaerobic fermentation, and add glycerin during the fermentation process to maintain the glycerin concentration in the fermentation system at 30g / L; control the culture temperature during the fermentation process to be 35°C, pH to be 7.0, stirring speed to be 400rpm...

Embodiment 3

[0046] (1) Strain cultivation: take 105mL of activated bacterial liquid, mix it with 595mL of seed medium in a 1L fermenter, and carry out micro-aerobic culture (without nitrogen inflow); the culture conditions are: culture temperature is 40°C, stirring speed is 300rpm , the pH was controlled at 7.0, and the fermented seed liquid was obtained after culturing for 18 hours.

[0047] (2) Continuous fermentation: The volume of the fermenter is 15L, and the liquid volume is 7L.

[0048] a. Take 700mL of fermented seed liquid and insert it into 6.3L of fermentation medium (medium-density chitosan with a molecular weight of 150,000 is added to the fermentation medium, and the addition amount is 3g / L).

[0049] b. Carry out anaerobic fermentation, and add glycerin during the fermentation process to maintain the glycerol concentration in the fermentation system at 35g / L; control the culture temperature during the fermentation process to be 40°C, pH to be 7.0, stirring speed to be 400rp...

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Abstract

The invention relates to a method for continuous production of 1,3-propanediol by microorganisms. The method includes culturing fermented strains through seed culture medium on the micro-aerobic condition to obtain fermented seed solution; inoculating the fermented seed solution to a fermentation medium to perform anaerobic fermentation, until concentration of 1,3-propanediol of the fermentation liquor is higher than 60g/L, performing micro-aerobic fermentation while controlling pH of the fermentation system to be acid, standing for separating, extracting supernatant liquid, adding in a supplemental medium while controlling pH of the fermentation system to be alkaline, repeating the step from the process of anaerobic fermentation, so as to realize continuous fermentation. The continuous fermentation mode can shorten fermentation time and improve production efficiency greatly.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for continuously producing 1,3-propanediol by using microorganisms. Background technique [0002] 1,3-Propanediol (1,3-Propanediol, PDO) is mainly used in the production of new polyester PTT (Polytrimethylene Terephthalate) with excellent performance. The polyester synthesized from it has unique properties and excellent performance, and can make polyester plastics biodegradable for easy natural circulation. In recent years, as an important organic synthesis raw material and intermediate, it has become a research and development hotspot because of its unique properties and wide range of uses. [0003] The industrial production methods of 1,3-propanediol mainly include chemical methods and biological methods. Biological method is the focus of current research, and can be divided into one-step glucose conversion method and glycerin conversion method acc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/22C12R1/01
CPCC12P7/18
Inventor 张霖樊亚超孙启梅李晓姝廖莎师文静
Owner CHINA PETROLEUM & CHEM CORP
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