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Fluorometric analysis method and device

A technology of fluorescence intensity and chromatographic membrane, which is applied in the direction of measuring devices, analyzing materials, and analyzing materials by optical means.

Inactive Publication Date: 2019-03-22
SHANGHAI XINPU BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This structure improves CV to a certain extent, but in medical testing, simply changing the structure of the bonding pad cannot meet the increasingly stringent clinical requirements

Method used

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  • Fluorometric analysis method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0175] Embodiment 1: the cv comparison of three kinds of test pieces of C-reactive protein (CRP) project Ea, Eb, Ec

[0176] 1. Preparation of standard curve:

[0177] 1) Eb test piece: Prepare series concentration CRP standard solution 1ng / mL, 5ng / mL, 10ng / mL, 25ng / mL, 50ng / mL, 100ng / mL with matrix solution. Take 70 μL of the series standard solution and mix with 10 μL of gold-labeled antibody (OD5), draw 60 μL dropwise to the sample pad of the membrane strip, and perform chromatography for 15 minutes to prepare the Eb test piece ( figure 2 ) standard curve.

[0178] 2) Ec test piece: take the complex solution mixed with 70 μL of the above-mentioned series of standard solutions and 10 μL (OD5) of the gold-labeled antibody, and make the solution contact with the opposite NC membrane end of the test piece absorbent pad according to the present invention for chromatography , 15min measurement, preparation of Ec test piece ( image 3 ) Standard curve of the test piece of the ...

Embodiment 2

[0184] Example 2: SA system data

[0185] 1. Preparation of standard curve: Prepare series concentration CRP standard solutions 1ng / mL, 5ng / mL, 10ng / mL, 25ng / mL, 50ng / mL, 100ng / mL with matrix solution.

[0186] a) Mix 70 μL of CRP standard solution with a series concentration and 10 μL (OD5) of gold-labeled antibody, then add bintin-CRP and mix evenly, absorb 60 μL dropwise to the sample pad of the film strip, and perform chromatography for 15 minutes to prepare the standard of Eb test piece curve.

[0187] b) Mix 70 μL of CRP standard solution with serial concentrations and 10 μL (OD5) of gold-labeled antibody, then add bintin-CRP and mix evenly, as the solution to be tested, make the solution and the opposite NC of the test piece absorbent pad according to the present invention Membrane end contacts carry out chromatography, 15min measures, prepares Ec test piece ( image 3 , the standard curve of the present invention).

[0188] 2. Select 5ng / mL and follow the above oper...

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Abstract

The invention relates to a fluorometric analysis method and device, in particular to a sample-free pad (preferably a combination-free pad), a test sheet based on fluorescence quenching immunochromatography principle and a detection method. An original immunochromatographic test strip structure is simplified; a sample pad is removed; the obvious reduction of a variation coefficient is accidentallydiscovered. The quantity of articles to be detected is determined by measuring the fluorescence intensity change of the test region; the specificity is high; the quantity of articles to be detected with the ultralow concentration can be detected; the articles to be detected can be simultaneously subjected to qualitative and quantitative analysis. The method has the advantages that the sensitivityis high; the quantitation is more accurate; the variable coefficient is lower; the stability is high; the operation is simple, convenient and fast; the cost is low; the method and the device are suitable for field fast qualitative and quantitative detection.

Description

technical field [0001] The invention belongs to the technical field of in vitro detection, and in particular relates to an analysis method and device based on measuring fluorescence intensity. Background technique [0002] In the field of immunoassay, qualitative or quantitative detection of various antigens or antibodies is often required. In the existing technology, a variety of immune response analysis methods are derived based on the "double-antibody sandwich", such as: radioimmunoassay, enzyme-linked immunoassay, chemiluminescence, time-resolved fluorescence and fluorescence immunoassay, etc., which can be used for The determination of pathogenic microorganisms, the quantitative detection of specific proteins in the human body, and the auxiliary diagnosis or monitoring of diseases, etc., have a wide range of uses. This type of immunoreaction analysis method usually immobilizes the capture antibody on a solid-phase carrier, then reacts with the antigen (target protein),...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/533
CPCG01N21/6428G01N33/533G01N2021/6432
Inventor 陈俊蕾张唯李久彤陈亮周雪雷
Owner SHANGHAI XINPU BIOTECH
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