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Chronic obstructive submaxillitis mouse model and preparation method thereof

A chronic obstructive, mouse model technology, applied in the field of biomedicine, can solve the problems of easy death of mice, many steps, and complexity, and achieve the effect of reducing the difficulty of modeling, reducing the risk of death, and ensuring integrity

Active Publication Date: 2019-03-26
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the method of ligating the submandibular gland ducts of mice is mostly used, but this method has many and complicated steps in the operation, because the skin of the mandible and neck needs to be cut, there is a risk of infection, the ligature line is easy to fall off, and the gland is easy to adhere to the mucous membrane after ligation , Postoperative mice are prone to death and other defects

Method used

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  • Chronic obstructive submaxillitis mouse model and preparation method thereof
  • Chronic obstructive submaxillitis mouse model and preparation method thereof

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Embodiment 1

[0024] This embodiment provides a mouse model of chronic obstructive submandibular gland inflammation and its preparation method. In this method, an angiosclerotic agent is injected into the middle of the mouth floor to cause aseptic necrosis of the local tissue, which is then gradually replaced by fibrous connective tissue. To block the duct opening of the submandibular gland, mice with chronic obstructive submandibular gland inflammation were obtained. Specific steps are as follows:

[0025] Take 8-week-old BALB / c mice, weighing 18±2g, inject 8 μL of absolute ethanol in the middle of the mouth floor with an oral anesthesia syringe, and then raise them normally for 7 days. The normal feeding method of mice is a routine technology that should be mastered by those skilled in the art, and will not be repeated here.

Embodiment 2

[0027] This embodiment provides a mouse model of chronic obstructive submandibular gland inflammation and its preparation method. In this method, an angiosclerotic agent is injected into the middle of the mouth floor to cause aseptic necrosis of the local tissue, which is then gradually replaced by fibrous connective tissue. To block the duct opening of the submandibular gland, mice with chronic obstructive submandibular gland inflammation were obtained. Specific steps are as follows:

[0028] Take 6-week-old ICR mice, weighing 20±2 g, inject 10 μL of absolute ethanol in the middle of the mouth floor with an oral anesthesia syringe, and then raise them normally for 15 days. The normal feeding method of mice is a routine technology that should be mastered by those skilled in the art, and will not be repeated here.

Embodiment 3

[0030] This embodiment provides a mouse model of chronic obstructive submandibular gland inflammation and its preparation method. In this method, an angiosclerotic agent is injected into the middle of the mouth floor to cause aseptic necrosis of the local tissue, which is then gradually replaced by fibrous connective tissue. To block the duct opening of the submandibular gland, mice with chronic obstructive submandibular gland inflammation were obtained. Specific steps are as follows:

[0031] Take 7-week-old C3H / He mice, weighing 24±2 g, inject 10 μL of sodium morrhuate injection in the middle of the mouth floor with an oral anesthesia syringe, and then raise them normally for 21 days. The normal feeding method of mice is a conventional technology that should be mastered by those skilled in the art, and will not be repeated here.

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Abstract

The invention discloses a chronic obstructive submaxillitis mouse model and a preparation method thereof, and relates to the field of biomedicine. According to the chronic obstructive submaxillitis mouse model and the preparation method thereof, an angiosclerosis agent is adopted for being injected to the middle portion of a mouth floor to make a local tissue generate aseptic necrosis, and then the local tissue is gradually replaced by a fibrous connective tissue, so that a submandibular gland duct opening is obstructed, and the purpose of modelling is achieved. The invention discloses the novel method for building the mouse chronic obstructive submaxillitis model. Compared with a traditional method, the novel method for building the mouse chronic obstructive submaxillitis model has the advantages that steps needed for modelling are reduced, the degree of difficulty of modelling is reduced, the integrity of a gland is guaranteed to the maximum extent, the risk of mouse mortality is reduced, and a reliable platform is provided for studying chronic obstructive submaxillitis.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a mouse model of chronic obstructive submandibular gland inflammation and a preparation method thereof. Background technique [0002] Chronic obstructive submaxillitis (COS) is a common clinical disease in oral and maxillofacial surgery. The main manifestation is recurrent swelling and pain of the submandibular gland, which can cause retrograde infection. The main causes of local obstruction are stones and inflammation. The diagnosis is mainly based on the patient's clinical signs, X-ray film, B-ultrasound and other methods, but it is more difficult to diagnose negative stones, or deep stones, and obstructive symptoms of unknown cause. [0003] COS is generally believed to be a chronic inflammatory process caused by reduced salivary flow due to intraductal and extraductal mechanical obstruction, with occasional retrograde infection. Although ductal obstruction is strongly associated...

Claims

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Application Information

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IPC IPC(8): A61K35/60A61K31/045
CPCA61K9/0019A61K31/045A61K35/60
Inventor 吴樱仪李春洁刘志凯曹钰彬付睿捷陈圣恺
Owner SICHUAN UNIV
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