30 results about "Fibrous connective tissue" patented technology

A new embolic agent, bioabsorbable polymeric material (BPM) is incorporated to a Guglielmi detachable coil (GDC) to improve long-term anatomic results in the endovascular treatment of intracranial aneurysms. The embolic agent, comprised at least in part of at least one biocompatible and bioabsorbable polymer and growth factors, is carried by hybrid bioactive coils and is used to accelerate histopathologic transformation of unorganized clot into fibrous connective tissue in experimental aneurysms. An endovascular cellular manipulation and inflammatory response are elicited from implantation in a vascular compartment or any intraluminal location. Thrombogenicity of the biocompatible and bioabsorbable polymer is controlled by the composition of the polymer. The coil further is comprised at least in part of a growth factor or more particularly a vascular endothelial growth factor, a basic fibroblast growth factor or other growth factors. The biocompatible and bioabsorbable polymer is in the illustrated embodiment at least one polymer selected from the group consisting of polyglycolic acid, poly˜glycolic acid / poly-L-lactic acid copolymers, polycaprolactive, polyhydroxybutyrate / hydroxyvalerate copolymers, poly-L-lactide. Polydioxanone, polycarbonates, and polyanhydrides.

A new embolic agent, bioabsorbable polymeric material (BPM) is incorporated to a Guglielmi detachable coil (GDC) to improve long-term anatomic results in the endovascular treatment of intracranial aneurysms. The embolic agent, comprised at least in part of at least one biocompatible and bioabsorbable polymer and growth factors, is carried by hybrid bioactive coils and is used to accelerate histopathologic transformation of unorganized clot into fibrous connective tissue in experimental aneurysms. An endovascular cellular manipulation and inflammatory response are elicited from implantation in a vascular compartment or any intraluminal location. Thrombogenicity of the biocompatible and bioabsorbable polymer is controlled by the composition of the polymer. The coil further is comprised at least in part of a growth factor or more particularly a vascular endothelial growth factor, a basic fibroblast growth factor or other growth factors. The biocompatible and bioabsorbable polymer is in the illustrated embodiment at least one polymer selected from the group consisting of polyglycolic acid, poly~glycolic acid / poly-L-lactic acid copolymers, polycaprolactive, polyhydroxybutyrate / hydroxyvalerate copolymers, poly-L-lactide. Polydioxanone, polycarbonates, and polyanhydrides.

Disclosed is a nervous tissue engineering tubular supporting stand and method for making same, wherein the nervous tissue engineering tubular supporting stand comprises a chitosan pipe wall and biological source filling base material with axial multiple passages, and the method consists of preparing semipermeable chitosan hollow pipe with 1-5mm of inside diameter, pouring large biological molecule solvent such as chitosan, collagen or gelatine, and utilizing special-purpose die arrangement and lyophilization technology. The obtained multiple-pass nerve channel with bionic construction is beneficial for cell adhesion, migration and leading of neuraxon directional growth, and suitable of the renovation and regeneration of nerve damages.

The invention provides a method for isolating and culturing human primary hepatocytes, comprising the following steps: cleaning blood clots on surfaces of hepatic tissues by using D-Hank's solution and cutting off connective tissues; using a needle syringe for puncturing on multipoints to perfuse anterior perfusion solution preheated to 38 DEG C so that a hepatic tissue block changes from dark red to gray and the effluent anterior perfusion solution becomes clear; using the needle for puncturing on multipoints to perfuse preheated recyclable type II collagenase solution until the hepatic tissue block is loosened and inelastic and has turtleback cracks on the surface; scissoring the hepatic tissue block, passively isolating the hepatic tissues, removing residual envelops and fibrillar connective tissues and continuously shaking and digesting in the type II collagenase solution at 37 DEG C; blowing and beating digest into hepatocyte suspension, filtering the suspension under ice bath condition, collecting filtered suspension, transferring the filtered suspension to a centrifuging tube and centrifuging at low speed; adding bottom precipitate produced from the primary low-speed centrifuging of the filtered suspension to erythrocyte lysate, blowing and beating, standing at room temperature, adding Dulbecco's modified eagle medium (DMEM), mixing the mixture uniformly, cleaning, centrifuging at low speed, adding DMEM again, mixing uniformly, centrifuging at low speed for 2-4 times and adding bottom precipitate produced from the last-time low-speed centrifuging to Williams' MediumE complete medium suspension; adjusting the hepatocytes density to 1*105 / ml, inoculating the hepatocytes in a culture bottle laid with rat tropocollagen, carrying out constant culturing in a CO2 incubator at 37 DEG C, changing the suspension to remove died hepatocytes and non-adherent hepatocytes after the hepatocytes are adhered to the wall and continually culturing the hepatocytes.

A new embolic agent, bioabsorbable polymeric material (BPM) is incorporated to a Guglielmi detachable coil (GDC) to improve long-term anatomic results in the endovascular treatment of intracranial aneurysms. The embolic agent, comprised at least in part of at least one biocompatible and bioabsorbable polymer and growth factors, is carried by hybrid bioactive coils and is used to accelerate histopathologic transformation of unorganized clot into fibrous connective tissue in experimental aneurysms. An endovascular cellular manipulation and inflammatory response are elicited from implantation in a vascular compartment or any intraluminal location. Thrombogenicity of the biocompatible and bioabsorbable polymer is controlled by the composition of the polymer. The coil further is comprised at least in part of a growth factor or more particularly a vascular endothelial growth factor, a basic fibroblast growth factor or other growth factors. The biocompatible and bioabsorbable polymer is in the illustrated embodiment at least one polymer selected from the group consisting of polyglycolic acid, poly˜glycolic acid / poly-L-lactic acid copolymers, polycaprolactive, polyhydroxybutyrate / hydroxyvalerate copolymers, poly-L-lactide. Polydioxanone, polycarbonates, and polyanhydrides.

The new-type spinal cord and peripheral nerve repairing material possesses same-direction microtube type structure characteristics, and can be made into the forms of cylinder and rectangle according to the requirements. It has the following characteristics: 1. exteral surface of said material is full-closed structure, can effectively preventing internal fibre connective tissue from growing into; 2. the microtube diameter size of the material can be controlled in 30-200 micrometers, it is favorable for growth of nerve regenerative fibre; and 3. arrangement directions of microtubes are axial, and mutual parallel and uniform. it is favourable of directional extension of nerve regenerative fibre. It can be dirctly implanted for repairing spinal cord and peripheral nerve defect, also can be used as carrier of various cell implantation, can carry various nerve growth factors and nerve trunk cell, etc.

The invention relates to a PEEK alveolar bone repairing material and an individualization producing method, and belongs to the field of polymer materials and biological medicine engineering. The PEEKalveolar bone repairing material is a polymer material which is composed of an outer compact layer and an inner loose layer, the compact layer can prevent peripheral soft tissues from growing into a bone defect area to form fibrous connective tissues, a bottom loose layer is provided with a through sponge porous structure, the hole structure gradually becomes larger from outside to inside, and thestructure can absorb and release active substances sustainably to promote the growth of bone tissues. A 3D printing technology is adopted by the material to form a repairing material which is highlymatched with the height of an alveolar bone. The PEEK alveolar bone repairing material has the advantages that the PEEK alveolar bone repairing material has better biological compatibility, the PEEK alveolar bone repairing material does not easily drop in the using process, can adapt to cold and hot environments in an oral cavity and can be individually produced according to the demands of a patient, the surgical operation time is greatly shortened, and the PEEK alveolar bone repairing material has good application prospects in alveolar bone regeneration of the department of stomatology.

Example inventions heal damage to a fibrous connective tissue. Examples affix a patch externally on a dermis of a user adjacent to a damaged fibrous connective tissue of the user, the patch comprising a flexible substrate, a processor directly coupled to the substrate, and electrodes directly coupled to the substrate. Examples then activate the patch, the activating comprising generating an electrical stimuli via the electrodes that is directed to the damaged fibrous connective tissue.

The invention provides a beef slide and a processing method thereof. The beef slide is composed of the following raw materials in percentage by weight: 80 to 85% of beef, 4 to 7% of cow grease particle, 2 to 4% of ice water, 1.5 to 2.5% of modified cassava starch, 1.5 to 2.5% of egg white, 1 to 2% of sugar, 0.75 to 1.25% of sesame oil, 1 to 2% of soybean sauce, 0.3 to 0.8% of salt, and 0.3 to 0.8% of monosodium glutamate. The processing method comprises the following steps: selecting and pre-processing the raw materials, and then subjecting the raw materials to the steps: 1, preserving; 2, grinding; 3, pulping; 4, cooling to form; and 5, packing the finished products so as to obtain the tender beef slide products. The beef slide has the advantages of delicious taste, more tender texture, and rich nutrients; the processing method maximally preserves the nutrients of raw materials, and fully breaks down the fibrous connective tissue of beef; thus the finished beef slide product has the characteristic of tenderness, smoothness, and crispness; moreover the processing method fully develops the potential value of beef products, increases the additional value, and satisfies the needs of market and nutritional needs of people in the modern society.

An embodiment of the invention relates to the technical field of scar preventing and treating and orthopedic treatment, in particular to an orthopedic tool for preventing and treating scars and a 3D manufacturing system and method of the orthopedic tool. The orthopedic tool manufactured by the 3D technology comprises an orthopedic tool body manufactured by the 3D technology. The orthopedic tool body comprises an inner structure and an outer structure covering the inner structure. A scar preventing and treating layer is arranged on the surface of the inner structure. The orthopedic tool body is formed by the 3D technology according to the contour of a scar area. When a patient wears the orthopedic tool body, the orthopedic tool body presses the burnt and scalded scar tissue of the scar area to limit the hyperplasia of fibrous connective tissue so as to prevent and treat scar hypertrophy, and orthopedic treatment and correcting are performed on the human joint functional disorder caused by scar contracture caused by the scar hypertrophy.

The invention discloses a method for promoting the multiplication of human adipose-derived stem cells. The method comprises the following steps: a fresh human adipose tissue is obtainedtaken, washed with a PBS (phosphate buffer solution), cut into pieces after adipose tissue membranes and blood vessels are removed, and digested, added into a complete medium is added after digestion is completed, undigested fibrous connective tissues are filtered out by a 200-mesh filter screen, centrifuging is carried out, supernate is discarded, suspended adipose cells and lipid droplets are removed, cells are precipitated and resuspended in complete medium, red blood cell lysisate buffer is added, the cells are cultured for lysis under normal temperature after uniform mixing, centrifuging is carried out,and supernate is removed; the cells are precipitated, resuspended in complete medium and inoculated into a culture flask, the culture solution is replaced and the non-adherent cells are removed afterbeing culture for 24 hours of culture, afterwards, solution is replaced every three days, and after being fused, the cells are digested and passage is carried out; the passage cells are extracted anddigested, so that cell suspension is prepared, the cell suspension is inoculated into a culture flask and cultured in complete medium, and after being cultured for 24 hours of culture, the complete medium is replaced by thujopsene-ketone-containing complete medium to continue culture.

The invention provides application of a collagen implant in preparing a glaucoma surgery auxiliary recovery substance, and belongs to the field of biomedical materials. The collagen is a cowhide purified collagen, and the recovery substance is a sponge-shaped collagen-chitosan matrix with a three-dimensional pore structure and the pore size distribution of 150-450 microns and serves as a surgery auxiliary recovery substance for glaucoma trabeculectomy. The recovery substance can promote the penetration of cells or aqueous humor or the discrete proliferation in the cells or aqueous humor to maintain low intraocular pressure and reduce the expression quantities of basic fibroblast growth factors, transforming growth factors and fibrous connective tissue growth factors to reduce the scarringdegree of a filtering tract; a preparation method of the recovery substance has the advantages that glutaraldehyde is not adopted as a cross-linking agent, the number of exposed amino groups in the chitosan structure can be reduced, the problem that the anti-scarring effect is not obvious due to the adhesion of excessive amino groups and the tissue is solved, the protective hydration function of chitosan chains is destroyed, pore channels in the matrix are smaller and compact, and the water absorption performance is improved.

The invention relates to an aqueous composition used in the laser repair treatment of fibrous connective tissue, including: gold nanoparticles in a concentration of between 1.5×109 particles per ml and 1×1010 particles per ml; and albumin and / or collagen in a concentration of between 30 wt % and 45 wt %. The invention also relates to an assembly including such a composition.

Disclosed is a nervous tissue engineering tubular supporting stand and method for making same, wherein the nervous tissue engineering tubular supporting stand comprises a chitosan pipe wall and biological source filling base material with axial multiple passages, and the method consists of preparing semipermeable chitosan hollow pipe with 1-5mm of inside diameter, pouring large biological molecule solvent such as chitosan, collagen or gelatine, and utilizing special-purpose die arrangement and lyophilization technology. The obtained multiple-pass nerve channel with bionic construction is beneficial for cell adhesion, migration and leading of neuraxon directional growth, and suitable of the renovation and regeneration of nerve damages.

The invention provides a medicine for wound repair and a preparation method thereof, and relates to the technical field of pharmaceutical preparations. The medicine is prepared from the following components in parts by weight: 0.2 to 0.8 part of ginsenoside Rb1, 4 to 12 parts of rhizoma chuanxiong, 15 to 25 parts of radix angelicae sinensis, 10 to 15 parts of semen euryales, 1 to 5 parts of rhizoma cimicifugae, 20 to 30 parts of rhizoma dioscoreae and 5 to 18 parts of rhizoma atractylodis macrocephalae. The medicine provided by the invention can restore the integrity and function of pelvic floor tissues from the structure by repairing the early pelvic floor tissue trauma after delivery and restoring the functions of pelvic floor muscles, fibrous connective tissues and the like, can prevent postpartum stress urinary incontinence and pelvic floor dysfunction diseases, and is good in curative effect and small in side effect. According to the preparation method, supercritical extraction and chromatographic column separation are combined for use, then the content of effective components of the medicinal materials is increased, the purity is improved, after the effective components of the medicinal materials and ginsenoside Rb1 are mixed, the repairing capacity of the medicine on pelvic floor muscles can be enhanced, and then the medicine effect is improved.

The invention discloses a bioactive bionic fiber connective tissue with a multistage ordered structure, a preparation method, a bionic tendon and a bionic ligament. The preparation method of the bioactive bionic fiber connective tissue comprises the following steps: mixing an active nano-material and bio-ink to obtain printing slurry, wherein the active nano-material contains osteogenic and / or tendon-forming active ions; carrying out 3D printing on the printing slurry to prepare a composite hydrogel scaffold; and stretching and drying the composite hydrogel scaffold to prepare the bioactive bionic fiber connective tissue. The preparation method can be used for preparing the bioactive bionic fiber connective tissue with a multistage ordered structure, has the advantages of simple preparation process and easiness in operation, and is beneficial to industrial popularization and application.

The presently disclosed subject matter relates to a peptide composition for non-covalently localizing fibrous connective tissue-inducing growth factor to a surface of an implant, the composition comprising a peptide having binding affinity for a surface material of an implant coupled to a peptide having binding affinity for a fibrous connective tissue-inducing growth factor. Methods are provided for delivering fibrous connective tissue-inducing growth factor GDF-7 in an amount effective to promote fibrous connective tissue repair and fibrous connective tissue formation. Also provided are methods of applying the peptide composition to an implant by contacting the composition with a surface of the implant; and kits comprising the compositions.

In the present invention, the biological TIMP-4 gene medicine contains complete cDNA sequence of TIMP-4 gene and the gene of PCINTIMP-4 vector, and TIMP-4 gene is assembled into specific expressing vector PcI-neo of mammal cell to subclone PCINTIMP-4. TIMP-4 gene protein has the powerful functions of inhibiting MMP enzyme, inhibit cell migration of smooth muscles, reducing the change of smooth muscle cell to become spindle cell and inhibiting the proliferation of connective fiber tissue in blood vessel cavity, so that it can inhibit abnormal repair course in blood vessel cavity for normal endothelium repair. The present invention has important application especially in preparing medicine for preventing blood vessel from becoming narrow again.

The invention discloses a hernia repairing piece which comprises a repairing piece body, wherein a magnet is arranged on the repairing piece body; a magnet containing ring is arranged on the repairingpiece body; the magnet is arranged inside the magnet containing ring. According to the hernia repairing piece disclosed by the invention, magnetic force is introduced as a mode of resisting pressureof organs, on one hand, the passive situation that the whole repairing piece is stressed is changed, the repairing force has large counteraction force at a weakest part, the suture requirement in a surgical operation is smartly reduced, and the requirement on the material of the repairing piece is also reduced; on the other hand, the pressure of the organs to a abdominal wall is reduced, the recovery of the abdominal wall can be helped, and fiber connective tissue cells can be infiltrated and adhered to the repairing piece to grow.

Sex hormone-binding globulin and / or any fragment thereof for use as a medicament, in particular for use in the treatment of obesity and hepatic steatosis. The invention also relates to the cosmetic use of the sex hormone-binding globulin and / or any fragment thereof for improving the bodily appearance of a mammal with subcutaneous fat herniated or accumulated within the fibrous connective tissue under the skin, in particular the cosmetic use is for cellulite.

The invention provides a tissue grinding device with a detachable filter screen. The tissue grinding device comprises a bearing device and a grinding device body, wherein a plurality of grinding cavities are formed in the bearing device, each grinding cavity is a cylinder, a grinding net is movably arranged in each grinding cavity, the grinding nets are movably connected with the bearing device through connecting devices, the grinding device body comprises a plurality of grinding rods, the grinding rods are movably arranged in the grinding cavities, and the grinding rods are in one-to-one correspondence with the grinding cavities. According to the tissue grinding device, a small number of a lysate is added into the grinding cavities, and the tissue lysate is obtained through grinding; anda single-cell suspension for flow type detection can also be prepared. The friction force is increased through the grinding nets, grinded tissue is not prone to move, the grinding nets are arranged tobe cylindrical, difficult-to-lyse fibrous connective tissue can be effectively isolated, the ground tissue is separated from the difficult-to-lyse fibrous connective tissue, the grinding nets and thegrinding cavities are of detachable structures, after the grinding is uniform, the grinding nets can be detached, and residual residues generated after the grinding and lysing can be removed conveniently so that the purity of the lysate or the single-cell suspension can be improved, and the practicability is high.

The invention provides grinding equipment for tissue homogenization. The grinding equipment comprises a bearing device and a grinding device, wherein the bearing device is a square body, and is provided with a plurality of grinding chambers which are cylindrical bodies and have different diameter dimensions, a grinding net is paved in each grinding chamber, and the diameter of the grinding net isthe same as the that of the grinding chamber. The grinding device comprises multiple grinding rods of various specifications, which move in the grinding chambers, and the diameters of the grinding rods correspond to those of the grinding chambers one by one. The grinding equipment for tissue homogenization has a simple structure, is convenient to operate, can achieve the effects of grinding, lysing, and homogenizing at the same time and obtain a tissue lysing solution by adding a small amount of lysing solution into the grinding chambers, and also can be used for preparing a single cell suspension for flow. The grinding nets increase friction, so that the tissue being ground is not easy to move; and the grinding nets can isolate the fibrous connective tissue that is difficult to lyse to achieve separation, the purity of the lysate or single cell suspension is improved, and the practicability is high.

The presently disclosed subject matter relates to a peptide composition for non-covalently localizing fibrous connective tissue-inducing growth factor to a surface of an implant, the composition comprising a peptide having binding affinity for a surface material of an implant coupled to a peptide having binding affinity for a fibrous connective tissue-inducing growth factor. Methods are provided for delivering fibrous connective tissue-inducing growth factor GDF-7 in an amount effective to promote fibrous connective tissue repair and fibrous connective tissue formation. Also provided are methods of applying the peptide composition to an implant by contacting the composition with a surface of the implant; and kits comprising the compositions.

The invention discloses a method for promoting multiplication of adipose-derived stem cells. The method comprises the following steps: collecting fresh human adipose tissue, flashing with a PBS (Phosphate Buffer Solution), removing adipose tissue membranes and blood vessels, cutting into pieces, digesting, placing into a complete culture medium after digestion is completed, filtering by using a filtering net till 200 meshes so as to remove undigested fibrous connective tissue, centrifuging, abandoning supernate, removing suspended fat cells and lipid droplets, re-suspending cell precipitate inthe complete culture medium, adding erythrocyte lysate, uniformly mixing, carrying out culture termination at a normal temperature, centrifuging, and abandoning supernate; re-suspending cell precipitate in the complete culture medium, inoculating into a culture bottle, culturing for 24 hours, replacing a culture liquid, removing non-adhered cells, and replacing the culture liquid every other three days till the cells are fused, digested and passed; digesting passage cells so as to obtain a cell suspension, inoculating into a culture bottle, culturing for 24 hours in the complete culture medium, and continuously culturing with a complete culture medium with hinesol or agarospirol.

The invention discloses a guided tissue regeneration membrane containing a TPE-NIM fluorescent probe and a preparation method of the guided tissue regeneration membrane. The preparation method comprises the following steps: (1) synthesizing TPE-Br; (2) synthesizing TPE-B (OH) 2; (3) synthesis of 4-bromine-N-(dimethylaminopropyl)-1, 8-naphthalimide is carried out; and (4) synthesizing the TPE-NIM. The GTR membrane which contains the slow-release antibacterial component, does not generate drug resistance and has bacterial traceability is used in a guided tissue regeneration technology (GTR), a membrane material is used as an isolation barrier, and the GTR membrane is placed at a periodontal tissue defect position to block gingival epithelium, prevent the gingival epithelium from growing along a root surface in a healing process and block fibrous connective tissue from being in contact with the surface of a tooth root, so that the healing effect of the gingival epithelium is improved. Therefore, periodontal ligament cells with the ability of forming new adhesion are guided to occupy the root surface preferentially, so that new adhesion is formed, and a growth space is provided for bone tissue regeneration.

The invention discloses a chronic obstructive submaxillitis mouse model and a preparation method thereof, and relates to the field of biomedicine. According to the chronic obstructive submaxillitis mouse model and the preparation method thereof, an angiosclerosis agent is adopted for being injected to the middle portion of a mouth floor to make a local tissue generate aseptic necrosis, and then the local tissue is gradually replaced by a fibrous connective tissue, so that a submandibular gland duct opening is obstructed, and the purpose of modelling is achieved. The invention discloses the novel method for building the mouse chronic obstructive submaxillitis model. Compared with a traditional method, the novel method for building the mouse chronic obstructive submaxillitis model has the advantages that steps needed for modelling are reduced, the degree of difficulty of modelling is reduced, the integrity of a gland is guaranteed to the maximum extent, the risk of mouse mortality is reduced, and a reliable platform is provided for studying chronic obstructive submaxillitis.

A device for performing a cosmetic or medical procedure and methods of operating such a device are usable for treating fibrous adhesions, such as those implicated in the formation of cosmetic or medical conditions including but not limited to cellulite. Embodiments of the disclosure have been particularly developed for targeting and disrupting fibrous adhesions to restore or improve normal physiological tissue function and / or aesthetic appearance. In at least one embodiment, the device comprises an elongate body and one or more user controllable breaking means. Each breaking means is actuatable to move between a retracted position in which the breaking means is in a collapsed configuration and an operative position in which the breaking means is in an expanded configuration suitable to break a strand of fibrous material or fibrous connective tissue.

The invention discloses a chronic obstructive submaxillitis mouse model and a preparation method thereof, and relates to the field of biomedicine. According to the chronic obstructive submaxillitis mouse model and the preparation method thereof, an angiosclerosis agent is adopted for being injected to the middle portion of a mouth floor to make a local tissue generate aseptic necrosis, and then the local tissue is gradually replaced by a fibrous connective tissue, so that a submandibular gland duct opening is obstructed, and the purpose of modelling is achieved. The invention discloses the novel method for building the mouse chronic obstructive submaxillitis model. Compared with a traditional method, the novel method for building the mouse chronic obstructive submaxillitis model has the advantages that steps needed for modelling are reduced, the degree of difficulty of modelling is reduced, the integrity of a gland is guaranteed to the maximum extent, the risk of mouse mortality is reduced, and a reliable platform is provided for studying chronic obstructive submaxillitis.

Sex hormone-binding globulin and / or any fragment thereof for use as a medicament, in particular for use in the treatment of obesity and hepatic steatosis. The invention also relates to the cosmetic use of the sex hormone-binding globulin and / or any fragment thereof for improving the bodily appearance of a mammal with subcutaneous fat herniated or accumulated within the fibrous connective tissue under the skin, in particular the cosmetic use is for cellulite.