Grinding equipment for tissue homogenization

Abstract

The invention provides grinding equipment for tissue homogenization. The grinding equipment comprises a bearing device and a grinding device, wherein the bearing device is a square body, and is provided with a plurality of grinding chambers which are cylindrical bodies and have different diameter dimensions, a grinding net is paved in each grinding chamber, and the diameter of the grinding net isthe same as the that of the grinding chamber. The grinding device comprises multiple grinding rods of various specifications, which move in the grinding chambers, and the diameters of the grinding rods correspond to those of the grinding chambers one by one. The grinding equipment for tissue homogenization has a simple structure, is convenient to operate, can achieve the effects of grinding, lysing, and homogenizing at the same time and obtain a tissue lysing solution by adding a small amount of lysing solution into the grinding chambers, and also can be used for preparing a single cell suspension for flow. The grinding nets increase friction, so that the tissue being ground is not easy to move; and the grinding nets can isolate the fibrous connective tissue that is difficult to lyse to achieve separation, the purity of the lysate or single cell suspension is improved, and the practicability is high.

Description

technical field [0001] The invention belongs to the technical field of cell processing, and in particular relates to a grinding device for tissue homogenization. Background technique [0002] Based on the structure and function of proteins and nucleic acids, understanding life phenomena at the molecular level has become the main direction of the development of modern biology. To study biological macromolecules, the first step is to obtain highly purified and biologically active target substances. The preparation of biomacromolecules involves all aspects of knowledge in physics, chemistry and biology. [0003] Microorganisms, plants and animals can all be used as raw materials for the preparation of biomacromolecules, and the selected materials are mainly determined according to the purpose of the experiment. For microorganisms, attention should be paid to its growth period. In the logarithmic growth period of microorganisms, the content of enzymes and nucleic acids is relat...

AI Technical Summary

Problems solved by technology

For example, the electric bead mill has a large area and is expensive; the hand-held homogenizer has a single function and can only process a single sample; the grinding bowl requires a large amount of lysate, and the grinding efficiency is low, so it is not suitable Processing of a large number of specimens; disposable grinding rod, highly targeted, low frequency of use, not suitable for general use

Another example is the most commonly used glass grinding homogenizer, which needs to be sterilized by soaking acid at high temperature and high pressure before use. If the sample volume is large, it will increase the labor load. In addition, during the grinding process, the lysate is prone to foaming, which increases the volume and is easy to lose.

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