Tissue grinding device with detachable filter screen

Abstract

The invention provides a tissue grinding device with a detachable filter screen. The tissue grinding device comprises a bearing device and a grinding device body, wherein a plurality of grinding cavities are formed in the bearing device, each grinding cavity is a cylinder, a grinding net is movably arranged in each grinding cavity, the grinding nets are movably connected with the bearing device through connecting devices, the grinding device body comprises a plurality of grinding rods, the grinding rods are movably arranged in the grinding cavities, and the grinding rods are in one-to-one correspondence with the grinding cavities. According to the tissue grinding device, a small number of a lysate is added into the grinding cavities, and the tissue lysate is obtained through grinding; anda single-cell suspension for flow type detection can also be prepared. The friction force is increased through the grinding nets, grinded tissue is not prone to move, the grinding nets are arranged tobe cylindrical, difficult-to-lyse fibrous connective tissue can be effectively isolated, the ground tissue is separated from the difficult-to-lyse fibrous connective tissue, the grinding nets and thegrinding cavities are of detachable structures, after the grinding is uniform, the grinding nets can be detached, and residual residues generated after the grinding and lysing can be removed conveniently so that the purity of the lysate or the single-cell suspension can be improved, and the practicability is high.

Description

technical field [0001] The invention belongs to the technical field of cell processing, in particular to a tissue grinding device with a detachable filter screen. Background technique [0002] Based on the structure and function of proteins and nucleic acids, understanding life phenomena at the molecular level has become the main direction of the development of modern biology. To study biological macromolecules, the first step is to obtain highly purified and biologically active target substances. The preparation of biomacromolecules involves all aspects of knowledge in physics, chemistry and biology. [0003] Microorganisms, plants and animals can all be used as raw materials for the preparation of biomacromolecules, and the selected materials are mainly determined according to the purpose of the experiment. For microorganisms, attention should be paid to its growth period. In the logarithmic growth period of microorganisms, the content of enzymes and nucleic acids is rela...

AI Technical Summary

Problems solved by technology

For example, the electric bead mill has a large area and is expensive; the hand-held homogenizer has a single function and can only process a single sample; the grinding bowl requires a large amount of lysate, and the grinding efficiency is low, so it is not suitable Processing of a large number of specimens; disposable grinding rod, highly targeted, low frequency of use, not suitable for general use

Another example is the most commonly used glass grinding homogenizer, which needs to be sterilized by soaking acid at high temperature and high pressure before use. If the sample volume is large, it will increase the labor load. In addition, during the grinding process, the lysate is prone to foaming, which increases the volume and is easy to lose.

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