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Methods and compositions for delivery of growth factor to fibrous connective tissue

A growth factor, fibrous knot technology, applied in growth factors/growth regulators, drug combinations, chemical instruments and methods, etc., can solve problems such as less success

Inactive Publication Date: 2010-09-08
AFFINERGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although a few technologies have been developed to deliver growth factors (such as growth factor-eluting polymers) and meet these delivery requirements, success remains less

Method used

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  • Methods and compositions for delivery of growth factor to fibrous connective tissue
  • Methods and compositions for delivery of growth factor to fibrous connective tissue
  • Methods and compositions for delivery of growth factor to fibrous connective tissue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Shown in this example are various methods for generating peptides with affinity for fibrous connective tissue-inducible growth factors of the present invention using phage display technology. A plurality of binding domain-containing peptides (i.e., peptides with affinity for implant surfaces and peptides with affinity for fibrous connective tissue-inducible growth factor) in the coating compositions of the invention were initially displayed using phage Technology development followed by improved binding characteristics through peptide design and peptide synthesis.

[0050] Phage screening and selection

[0051] Phage display techniques are well known in the art and can be used to attempt to identify phage displayed peptides that have affinity for a particular target substrate used in the screen. Typically, using phage display, a library of various peptides against a target substrate can be provided, and a peptide that specifically binds to the substrate can be select...

Embodiment 2

[0063] In this example, the identification of the peptides of the invention is further shown. The peptides of the present invention can be synthesized using any method known to those skilled in the art, including but not limited to solid phase synthesis, solution phase synthesis, linear synthesis and combinations thereof. In this example, peptides were synthesized using standard solid phase peptide synthesis techniques on a peptide synthesizer using standard Fmoc chemistry. After all residues have been coupled, cleavage and side chain deprotection are performed simultaneously by treatment with complex trifluoroacetic acid (TFA). The crude peptide was precipitated with cold diethyl ether and purified by high performance liquid chromatography (HPLC) using a linear water / acetonitrile gradient with 0.1% TFA. The homogeneity of the synthesized peptides was assessed by analytical reverse-phase-HPLC, and the identity of the peptides was confirmed by mass spectrometry.

[0064] af...

Embodiment 3

[0073] Table 3 shows exemplary peptides having affinity for implant surface materials ("surface-bound peptides"). For example, surface-binding peptides include those having a surface-binding region and are sensitive to, for example, polystyrene, polyurethane, polyglycolic acid, polycarbonate, nylon, Materials such as polyethylene terephthalate fibers and collagen-based substrates have affinity for peptides. Exemplary surface-binding peptides include SEQ ID NOs: 13-71. In certain embodiments, at least one surface-binding peptide may be conjugated to at least one peptide having an affinity for fibrous connective tissue-inducible growth factor in forming the compositions described herein.

[0074] table 3

[0075]

[0076]

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PUM

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Abstract

The presently disclosed subject matter relates to a peptide composition for non-covalently localizing fibrous connective tissue-inducing growth factor to a surface of an implant, the composition comprising a peptide having binding affinity for a surface material of an implant coupled to a peptide having binding affinity for a fibrous connective tissue-inducing growth factor. Methods are provided for delivering fibrous connective tissue-inducing growth factor GDF-7 in an amount effective to promote fibrous connective tissue repair and fibrous connective tissue formation. Also provided are methods of applying the peptide composition to an implant by contacting the composition with a surface of the implant; and kits comprising the compositions.

Description

[0001] Cross References to Related Applications [0002] This application claims priority to US Provisional Application Patent Serial No. 60 / 969,748, filed September 4, 2007; the disclosure of which is hereby incorporated by reference in its entirety. [0003] funding statement [0004] This invention was made in part with government support under Grant No. 1R43AR053753-01 from the National Institute of Arthritis and Musculoskeletal and Skin Diseases. Accordingly, the US Government has certain rights in this invention. technical field [0005] The subject matter of the present invention relates to compositions for the delivery of growth factors to fibrous connective tissue. More specifically, the present invention relates to a family of peptides having affinity for growth factors important for regeneration of fibrous connective tissue, which peptides are associated with one or more implants for repairing fibrous connective tissue defects or inducing fibrous connective tissue...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/02A61K38/04
CPCC07K14/71A61L2300/25C07K2319/20A61L2300/414A61K9/0024A61L27/227A61K38/00A61L27/54A61L2300/606A61P19/04
Inventor 本杰明·马库斯·比勒保罗·西奥多·汉密尔顿达莉亚·伊索尔德·祖米尼师库玛·阿布杰克森·奈尔陈玉琛
Owner AFFINERGY INC
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