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Method for promoting multiplication of adipose-derived stem cells

A technology of human adipose stem cells and cells, which is applied in the field of biological stem cells, can solve the problems that have not yet been seen

Inactive Publication Date: 2019-01-08
淮安康宜莱干细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] So far, there is no report that maoshuol, agarwood spirocycline and rohanbocenone can promote the proliferation of adipose-derived stem cells

Method used

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  • Method for promoting multiplication of adipose-derived stem cells
  • Method for promoting multiplication of adipose-derived stem cells
  • Method for promoting multiplication of adipose-derived stem cells

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0020] The following describes the substantive content of the present invention in detail in conjunction with the drawings and embodiments, but does not limit the protection scope of the present invention.

[0021] 1. Experimental materials

[0022] Fresh human adipose tissue was obtained from patients undergoing abdominal liposuction, without infectious or endocrine diseases.

[0023] High-glucose DMEM medium and fetal bovine serum were purchased from Gibco, USA. Majool, agarwood spirool and phoenixenone are self-made or purchased, and the purity is greater than 98%.

[0024] Cell counting kit (CCK-8) was purchased from Dojin Chemical Research Institute of Japan Co., Ltd.

[0025] 2. Experimental method

[0026] 1. Isolation and culture of adipose-derived stem cells

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Abstract

The invention discloses a method for promoting multiplication of adipose-derived stem cells. The method comprises the following steps: collecting fresh human adipose tissue, flashing with a PBS (Phosphate Buffer Solution), removing adipose tissue membranes and blood vessels, cutting into pieces, digesting, placing into a complete culture medium after digestion is completed, filtering by using a filtering net till 200 meshes so as to remove undigested fibrous connective tissue, centrifuging, abandoning supernate, removing suspended fat cells and lipid droplets, re-suspending cell precipitate inthe complete culture medium, adding erythrocyte lysate, uniformly mixing, carrying out culture termination at a normal temperature, centrifuging, and abandoning supernate; re-suspending cell precipitate in the complete culture medium, inoculating into a culture bottle, culturing for 24 hours, replacing a culture liquid, removing non-adhered cells, and replacing the culture liquid every other three days till the cells are fused, digested and passed; digesting passage cells so as to obtain a cell suspension, inoculating into a culture bottle, culturing for 24 hours in the complete culture medium, and continuously culturing with a complete culture medium with hinesol or agarospirol.

Description

technical field [0001] The invention belongs to the field of biological stem cells, and relates to a method for promoting the proliferation of human fat stem cells. Background technique [0002] Adipose stem cells are a type of pluripotent stem cells in adipose tissue, which can differentiate into various cell types under specific conditions in vivo and in vitro, such as adipocytes, osteoblasts, chondrocytes, nerve cells, muscle cells, etc. At present, adipose stem cell-assisted free autologous fat transplantation has been proven to effectively improve the survival rate of transplanted fat. Compared with bone marrow stromal-derived stem cells, adipose-derived stem cells are considered to be the best choice for cell-assisted fat transplantation, because a large number of adipose-derived stem cells can be obtained through liposuction. As a precursor of adipose tissue, adipose-derived stem cells are involved in various adipose tissue remodeling, including growth and developmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0667C12N2501/999C12N2509/00
Inventor 赵立士陈名星
Owner 淮安康宜莱干细胞生物科技有限公司
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