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Method for fermentation production of folic acid

A folic acid and fermentation liquid technology, applied in the field of fermentation engineering, can solve the problems of unsuitable industrial production, unfavorable industrial production, high production cost of folic acid, etc.

Inactive Publication Date: 2019-03-26
CHANGZHOU XINHONG PHARMA & CHEM IND TECHOLOGIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Natural folic acid is mainly derived from green plant leaves, animal liver, egg yolk, soybean and wheat, but by extracting folic acid from folic acid-rich raw materials such as green plant leaves, animal liver, egg yolk, soybean and wheat, it will make folic acid The production cost is huge, and it is not suitable for industrial production. At the same time, the high price caused by the production cost will also greatly limit the market of folic acid. Therefore, it is urgent to find a method that can greatly reduce the production cost of folic acid.
[0006] Fortunately, at present, studies have found that some microorganisms, such as yeast and lactic acid bacteria, have the ability to ferment and produce folic acid. If folic acid can be produced through biological fermentation, it will undoubtedly solve the problem of high folic acid production costs.
[0007] However, yeast, lactic acid bacteria and other microorganisms that can produce folic acid have limited folic acid production capacity, generally only 0.5-2 μg / mL, which is not conducive to industrial production. Therefore, it is very important to find a method that can improve the ability of microorganisms to produce folic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Specific steps are as follows:

[0038] (1) Draw 0.3mL seed culture medium and put it into the Candida utilis strain tube purchased from the China Industrial Microorganism Culture Collection Management Center, shake gently to obtain the bacterial suspension;

[0039] (2) Put 50mL seed medium in a 250mL shake flask, and sterilize at 121°C for 20min;

[0040] (3) Inoculate 0.3 mL of the bacterial suspension into the seed culture medium, and cultivate for 12 hours at a temperature of 28° C., a natural pH, and a rotational speed of 200 rpm to obtain a seed liquid;

[0041] (4) Put 100mL fermentation medium in a 500mL shake flask, and sterilize at 121°C for 20min;

[0042] (5) Inoculate the seed liquid in the fermentation medium with an inoculation amount of 5%, and ferment and cultivate it for 18 hours at a temperature of 28° C., a pH of 7, and a rotation speed of 180 rpm;

[0043] (6) After 18 hours, 20 g / L lactose and 3 g / L lactic acid were added to the fermentation cul...

Embodiment 2

[0046] Specific steps are as follows:

[0047] (1) Draw 0.3mL seed culture medium and put it into the Candida utilis strain tube purchased from the China Industrial Microorganism Culture Collection Management Center, shake gently to obtain the bacterial suspension;

[0048] (2) Put 50mL seed medium in a 250mL shake flask, and sterilize at 121°C for 20min;

[0049](3) Inoculate 0.3 mL of the bacterial suspension into the seed culture medium, and cultivate for 8 hours at a temperature of 28° C., a natural pH, and a rotational speed of 200 rpm to obtain a seed liquid;

[0050] (4) Put 100mL fermentation medium in a 500mL shake flask, and sterilize at 121°C for 20min;

[0051] (5) Inoculate the seed liquid in the fermentation medium with an inoculation amount of 3%, and ferment and cultivate it for 16 hours at a temperature of 28° C., a pH of 7, and a rotation speed of 180 rpm;

[0052] (6) After 18 hours, 20 g / L lactose and 3 g / L lactic acid were added to the fermentation cultu...

Embodiment 3

[0055] Specific steps are as follows:

[0056] (1) Draw 0.3mL seed culture medium and put it into the Candida utilis strain tube purchased from the China Industrial Microorganism Culture Collection Management Center, shake gently to obtain the bacterial suspension;

[0057] (2) Put 50mL seed medium in a 250mL shake flask, and sterilize at 121°C for 20min;

[0058] (3) Inoculate 0.3 mL of the bacterial suspension into the seed culture medium, and cultivate for 12 hours at a temperature of 28° C., a natural pH, and a rotational speed of 200 rpm to obtain a seed liquid;

[0059] (4) Put 100mL fermentation medium in a 500mL shake flask, and sterilize at 121°C for 20min;

[0060] (5) Inoculate the seed solution in the fermentation medium with an inoculation amount of 10%, and ferment and cultivate for 22 hours at a temperature of 28° C., a pH of 7, and a rotation speed of 180 rpm;

[0061] (6) After 18 hours, 20 g / L lactose and 3 g / L lactic acid were added to the fermentation cul...

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PUM

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Abstract

The invention discloses a method for fermentation production of folic acid and belongs to the technical field of fermentation engineering. The method comprises the steps of firstly inoculating candidautilis in a fermentation medium; carrying out fermentation culture for 16-22 hours at the temperature of 20-30 DEG C and at pH 6-8; adding lactose and / or lactic acid in the fermentation medium; and continuing the fermentation culture for 20-30 hours at the temperature of 5-15 DEG C and at pH 4.5-6 to increase the yield of the folic acid produced by fermentation. By utilizing the method disclosedby the invention, the yield of the folic acid produced by fermentation can be increased from 2.2 micrograms per milliliter to 4.9 micrograms per milliliter.

Description

technical field [0001] The invention relates to a method for producing folic acid by fermentation, which belongs to the technical field of fermentation engineering. Background technique [0002] Folic acid (folate) belongs to the water-soluble vitamin B group and is a general term for a class of compounds with pteroyl glutamic acid molecular structure, also known as pteroyl glutamic acid, vitamin Bc, vitamin M, vitamin B11, and its parent pteroyl glutamic acid The chemical name of the acid is N-[4-[(2-amino-4-hydroxy-6-pteridinyl)methylamino]benzoyl]-L-glutamic acid. [0003] The important physiological function of folic acid is to participate in the metabolism as a carrier of one-carbon compounds. Its derivative tetrahydrofolate acts as a carrier of one-carbon units (including formate, formaldehyde and methyl) in the form of coenzymes. The utilization of formaldehyde plays an important role. [0004] Since the folic acid in the human body is almost completely dependent on...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/72
CPCC12P17/182
Inventor 杨可武宋烨航郑东晖
Owner CHANGZHOU XINHONG PHARMA & CHEM IND TECHOLOGIES