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Method for preparing ginseng polypeptide by double-enzyme step-by-step enzymatic hydrolysis

A step-by-step enzymatic hydrolysis and double-enzymatic enzymatic hydrolysis technology is applied in the field of preparation of ginseng polypeptides, which can solve the problems of low protein absorption rate and cannot meet the needs of protein, and achieve high polypeptide purity, good application prospects, and green environmental protection efficiency. Effect

Inactive Publication Date: 2019-03-26
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the low absorption rate of protein has become the main limiting factor, and the method of simply ingesting protein through food can no longer meet the human body's demand for protein.

Method used

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  • Method for preparing ginseng polypeptide by double-enzyme step-by-step enzymatic hydrolysis
  • Method for preparing ginseng polypeptide by double-enzyme step-by-step enzymatic hydrolysis
  • Method for preparing ginseng polypeptide by double-enzyme step-by-step enzymatic hydrolysis

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Effect test

Embodiment 1

[0024] The embodiment of the present invention provides a preparation method of ginseng polypeptide, the specific steps are as follows:

[0025] A. Wash the ginseng with ultra-pure water, cut it into pieces after fully drying, grind it with a high-speed tissue grinder, and pass the obtained ginseng powder through an 80-mesh sieve.

[0026] B. Add PBS buffer solution according to the mass volume ratio of 1:10, sonicate with a sonicator for 30 minutes at room temperature, and stir and extract at 4°C for 12 hours. The obtained extract was centrifuged at 5000rpm for 30min, and the supernatant was retained. According to the ratio of mass volume ratio of 1:10, PBS buffer solution was added to the precipitate, stirred and leached for a second time at 4°C for 12h, and the obtained extract was centrifuged at 5000rpm for 30min. The two supernatants were combined to obtain the crude extract of ginseng protein.

[0027] C. Add the crude extract of ginseng protein into pre-cooled acetone...

Embodiment 2

[0031] The embodiment of the present invention provides a preparation method of ginseng polypeptide, the specific steps are as follows:

[0032] A. Wash the ginseng with ultra-pure water, cut it into pieces after fully drying, grind it with a high-speed tissue grinder, and pass the obtained ginseng powder through an 80-mesh sieve.

[0033] B. Add PBS buffer solution according to the mass volume ratio of 1:20, sonicate with a sonicator for 60 minutes at room temperature, and stir and extract at 4°C for 20 hours. The obtained extract was centrifuged at 5000rpm for 30min, and the supernatant was retained. According to the ratio of mass volume ratio of 1:20, PBS buffer solution was added to the precipitate, stirred and leached for a second time at 4°C for 20 h, and the obtained extract was centrifuged at 5000 rpm for 30 min. The two supernatants were combined to obtain the crude extract of ginseng protein.

[0034] C. Add pre-cooled acetone to the protein extract according to the ...

Embodiment 3

[0038] The embodiment of the present invention provides a preparation method of ginseng polypeptide, the specific steps are as follows:

[0039] A. Wash the ginseng with ultrapure water, cut it into pieces after sufficient drying, and pulverize it with a high-speed tissue grinder, and pass the obtained ginseng powder through an 80-mesh sieve.

[0040] B. Add PBS buffer solution according to the mass volume ratio of 1:20, sonicate with a sonicator for 60 minutes at room temperature, and stir and extract at 4°C for 20 hours. The obtained extract was centrifuged at 5000rpm for 30min, and the supernatant was retained. According to the ratio of mass volume ratio of 1:20, PBS buffer solution was added to the precipitate, stirred and leached for a second time at 4°C for 20 h, and the obtained extract was centrifuged at 5000 rpm for 30 min. The two supernatants were combined to obtain the crude extract of ginseng protein.

[0041] C. Add pre-cooled acetone to the protein extract acc...

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Abstract

The invention relates to a method for preparing ginseng polypeptide by double-enzyme step-by-step enzymatic hydrolysis, and belongs to the technical field of polypeptide preparation by enzymatically hydrolyzing vegetable protein. The method comprises the steps ginseng powder is used as a raw material, and the ginseng polypeptide is prepared by preparing a crude ginseng protein extract, preparing atotal ginseng protein and performing double-enzyme enzymatic hydrolysis; and the double-enzyme enzymatic hydrolysis is performed, specifically, flavourzyme with the enzyme activity of 20U / mg and alkaline protease with the enzyme activity of 200U / mg are mixed with equal amount and enzymatically hydrolyzed for 4-6h, or separated with equal amount and enzymatically hydrolyzed with the alkaline protease for 4-6 h and enzymatically hydrolyzed with the flavourzyme for 20 h. The method is reasonable, the process is simple, the operation is easy, the pH value and temperature do not need to be repeatedly adjusted, the enzymatic hydrolysis effect is obvious, the purity of the polypeptide is high, and the yield is high and the loss is small; and the extracted ginseng polypeptide has good safety, andhas good application prospects in food, medicine, health care products, cosmetics and other fields.

Description

technical field [0001] The invention belongs to the technical field of step-by-step enzyme hydrolysis of plant protein to prepare polypeptides, and in particular relates to a preparation method of ginseng polypeptides. Background technique [0002] Ginseng (Panax ginseng C.A.Mey) is known as the "King of Herbs". It is an authentic medicinal material in Northeast China. Since it has been recorded, "Shen Nong's Materia Medica" first described the pharmacological effects of ginseng: it can nourish the five internal organs, calm the spirit, calm the soul, stop palpitations, improve eyesight, and improve intelligence. The nutritional components of ginseng mainly include amino acids, proteins, sugars, volatile oils, nucleotides, flavonoids, ginsenosides, organic acids, vitamins, sterols and inorganic elements. Modern medical research shows that, as the main active ingredient, ginsenosides are widely involved in the central nervous system, stress and sexual functions, as well as r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 胡良海程显惠李潇影张贵元杨晓敏
Owner JILIN UNIV
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