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Method for determining free amino acid in biogas slurry

A technology of free amino acids and biogas slurry, applied in the direction of color/spectral characteristic measurement, etc., can solve the problems of undisclosed amino acid content, large differences in biogas slurry components, and difficult processing of biogas slurry products, and achieve accurate, repeatable and reliable experimental analysis. good stability effect

Inactive Publication Date: 2019-03-26
ZHEJIANG UNIV OF TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

However, different raw materials and different seasons lead to great differences in biogas slurry composition, which brings great difficulties to the processing of biogas slurry products. Therefore, the analysis of different batches of biogas slurry components is particularly important. Determining its content is of great significance for the development and utilization of biogas slurry products
At present, the determination and analysis method of amino acid content in biogas slurry has not been disclosed

Method used

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  • Method for determining free amino acid in biogas slurry
  • Method for determining free amino acid in biogas slurry

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Experimental program
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Embodiment 1

[0024] (1) Sample processing

[0025] Take 40mL biogas slurry in a 50mL plastic centrifuge tube, centrifuge (8000rpm, 4°C, 15min, centrifuge 3 times), take the supernatant and filter (0.22μm PTFE filter membrane), then adjust the pH to 10.2 with 1mol / L NaOH solution, Then add it to a single-necked round-bottomed flask, evaporate it to dryness at 40°C and 80rmp, redissolve it in 5mL ultrapure water, and ultrasonically induce dissolution for 5 minutes to obtain a biogas slurry concentrate that removes high-concentration ammonia and volatile bioorganic amines 5mL, spare;

[0026] (2) Prepare ninhydrin solution, isoleucine standard solution, acetic acid-sodium acetate buffer solution;

[0027] The preparation method of described ninhydrin solution is: take by weighing 0.6g of ninhydrin (purity≥99%), dissolve with 15mL analytical pure n-propanol, 30mL analytical pure n-butanol, 60mL analytical pure ethylene glycol, then add 9mL Acetic acid-sodium acetate buffer;

[0028] The pre...

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Abstract

The invention provides a method for determining free amino acid in biogas slurry. According to the method disclosed by the invention, a simple high-efficiency analysis method is utilized, and the optimal reaction condition is determined; thus, experimental analysis has the advantages of good accuracy, sensitivity, quickness and repeatability and stability, and theoretical basis is provided for further research on composition of amino acid in the biogas slurry and development and utilization of the biogas slurry.

Description

(1) Technical field [0001] The invention relates to a method for measuring free amino acids in biogas slurry, which belongs to the field of component analysis and environmental resource reuse. (2) Background technology [0002] With the rapid development of society and the continuous improvement of people's living standards, the demand for meat products is also increasing, which promotes the intensive and large-scale development of the livestock and poultry breeding industry, which will inevitably lead to a sharp increase in the production of livestock and poultry wastewater. Livestock and poultry wastewater containing high concentrations of COD and ammonia nitrogen pose great challenges to traditional biological treatment methods. The more typical one is pig raising wastewater. On the one hand, this type of wastewater has complex components and emits foul odor. If it is discharged directly without treatment, it will bring certain environmental risks. On the other hand, this...

Claims

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Application Information

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IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 戴启洲郑天翔邱宗炼陈建孟
Owner ZHEJIANG UNIV OF TECH