Application of methidazine in improving progestogen resistance of endometrial cancer
A technology of endometrial cancer and thioridazine is applied in the application field of thioridazine in improving progesterone resistance of endometrial cancer, which can solve problems such as drug resistance, achieve improving progesterone resistance, promoting The effect of inhibiting proliferation and promoting apoptosis
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Embodiment 1
[0024] Example 1. The experiment of thioridazine combined with medroxyprogesterone acetate inhibiting the proliferation and viability of endometrial cancer cells
[0025] The materials are as follows:
[0026] Cells: endometrial cancer cell lines Ishikawa, KLE; drugs: thioridazine, medroxyprogesterone acetate (MPA)
[0027] Method: Cells in the logarithmic growth phase with a density of 8×103 cells / well were inoculated in a 96-well plate for culture. After 24 hours, different concentrations of MPA (0, 1, 10, 20, 30, 40, 50 μmol / L) were added. Concentration gradients were used to set 6 parallel wells, and then placed in a 37° C., 5% CO2 incubator for 24 hours to conduct experiments.
[0028] The cells in the logarithmic phase of growth were collected, and the drugs were divided into groups according to the experiment. Six parallel wells were set up in each group, and they were cultured for 1, 2, 3, and 4 days respectively for the experiment. Calculate the inhibition rate of...
Embodiment 2
[0030] Example 2. The experiment of thioridazine combined with medroxyprogesterone acetate inhibiting the apoptosis of endometrial cancer cells
[0031] The materials are as follows:
[0032] Method: The cells in the logarithmic growth phase were routinely digested. After 24 hours of inoculation, the cells were replaced with serum-free medium and cultured for 18 hours. After adding the experimental drug for 72 hours (drug treatment grouping was the same as in Example 1), conventional EDTA-free trypsin digestion was used, and cold PBS was used. buffer to wash the cells twice, and then make 1×10 with 1×Binding Buffer 6 For single cell suspension, centrifuge to discard the supernatant, then wash twice with pre-cooled phosphate buffered saline (PBS), add 100 μl of cell suspension to the Falcon test tube, add AnnexinV-FITC and 5 μl of accounting dye (PI) respectively , mixed gently, and placed in a dark place at room temperature for 15 minutes. Add 400 μl of 1×Binding Buffer to...
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