Method for researching influence of exogenous selenium on selenium content, glutathione peroxidase activity, and quality of tea

A technology for glutathione peroxidase and selenium content, which can be used in measuring devices, instruments, scientific instruments, etc., and can solve the problems of tea glutathione peroxidase activity and quality influence.

Inactive Publication Date: 2019-03-29
AGRI RESOURCE & ENVIRONMENT RES INST GUANGXI ACADEMY OF AGRI SCI
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Problems solved by technology

[0003] The invention provides a research method for the influence of exogenous selenium on tea selenium content, glutathione peroxidase activity and quality, so as to solve the problem of selenium's influence on tea glutathione peroxidase activity and quality. The problem

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  • Method for researching influence of exogenous selenium on selenium content, glutathione peroxidase activity, and quality of tea
  • Method for researching influence of exogenous selenium on selenium content, glutathione peroxidase activity, and quality of tea
  • Method for researching influence of exogenous selenium on selenium content, glutathione peroxidase activity, and quality of tea

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Embodiment Construction

[0023] 1 Materials and methods

[0024] 1.1 Test materials

[0025] The test site is located at 109°56′59″ east longitude, 23°18′36″ north latitude, and 46 meters above sea level in Guangxi Guiping Bishui Xishan tea garden base. The tested variety is Jinxuan tea, which is 14 years old. The physical and chemical properties of the soil are listed in Table 1.

[0026] Table 1 Soil physical and chemical properties

[0027]

[0028] 1.2 Test method

[0029] Test settings 0, 9, 12, 15, 18, 21L / hm 2 , the concentration gradient of 6 amino acid chelated selenium liquid fertilizers, after the fertilizer is mixed with water, choose cloudy days for drenching, set 3 repetitions, and the area of ​​each plot is 50m 2 . 30 days after selenium application, two leaves and one heart were picked, rinsed with distilled water three times, dried with filter paper, and fresh leaves were taken for the determination of antioxidant enzymes. The remaining leaves were killed at 105°C for 5 minute...

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Abstract

The invention discloses a method for researching influence of exogenous selenium on selenium content, glutathione peroxidase activity, and quality of tea. The method comprises the following steps of:(1) setting a concentration gradient of 6 amino acid chelated selenium liquid fertilizer in a test, choosing cloudy day for leaching after the fertilizer is mixed with water, and setting 3 repetitions, wherein the area of each plot is consistent; (2) plucking leaves with two leaves and one core after applying selenium, rinsing 3 times with distilled water, drying with filter paper, taking fresh leaves to measure antioxidant enzymes, performing the deactivation of enzymes on the remaining leaves, and then drying, and smashing and sifting to obtain tea pulverized materials; and (3) using the teapulverized materials obtained in the step 2 to determine the selenium content, glutathione peroxidase activity, tea polyphenol content, and tea polysaccharide content of the tea. The invention makesa preliminary discussion on the influence of exogenous selenium on the selenium content, glutathione peroxidase activity, and quality of tea, and provides a theoretical basis so as to further study the dynamic changes of selenium in tea and the regulation of selenium on physiological mechanisms of tea.

Description

technical field [0001] The invention belongs to the technical field of selenium regulating tea physiological mechanism, and specifically relates to a research method for the influence of exogenous selenium application on tea selenium content, glutathione peroxidase activity and quality. Background technique [0002] Selenium (Se) is one of the 15 essential trace elements for the human body, and also one of the non-metallic trace elements related to various diseases of the human body. In 1973, selenium was discovered and proved to be a component of glutathione peroxidase, which is an important selenium-containing antioxidant enzyme, which can effectively remove active oxygen and block the chain reaction of lipid peroxidation. To ensure the normal progress of the life activities of organisms. 72% of my country's areas are selenium-deficient or low-selenium areas, and the daily intake of selenium in low-selenium areas is significantly lower than the intake recommended by the W...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00
CPCG01N33/00
Inventor 梁潘霞刘永贤邢颖廖青潘丽萍陈锦平江泽普
Owner AGRI RESOURCE & ENVIRONMENT RES INST GUANGXI ACADEMY OF AGRI SCI
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